Relaxation Effect of Schisandrol A on Isolated Thoracic Aorta and its Mechanism in Rats DOI Creative Commons

Xudong Qiu,

Dong Yang,

Bi-Han Wang

et al.

Pharmacognosy Magazine, Journal Year: 2023, Volume and Issue: 20(2), P. 485 - 493

Published: Oct. 19, 2023

Background Schisandra chinensis (S. chinensis) is a drug commonly used in the clinical treatment of cardiovascular diseases Traditional Chinese Medicine. However, specific components and mechanisms its action are still unclear. We screened six kinds lignans from S. with high content found that schisandrol A schisantherin had strong vasorelaxant effect. The purpose this study was to investigate relaxation underlying mechanism isolated thoracic aorta rats. Materials Methods Isolated rat endothelium-intact endothelium-removed strips were pre-constricted phenylephrine (PE), on observed. Then, explored by pre-incubating nitric oxide synthetase inhibitor Nɷ-nitro-l-arginine methyl ester (L-NAME), cyclooxygenase (indomethacin), potassium channel blockers 4-aminopyridine (4-AP), barium chloride (BaCl2), tetraethylamine (TEA), glibenclamide, respectively, changing calcium concentration bath. In addition, expressions endothelial (eNOS) mRNA protein detected. Results Schisandrol induced both endothelium-dependent endothelium-independent rats, might be related promoting synthesis NO, inhibiting Ca 2+ release sarcoplasmic reticulum, blocking channels. Conclusion These discoveries may provide theoretical basis for traditional application treat disease.

Language: Английский

The mechanisms, regulations, and functions of histone lysine crotonylation DOI Creative Commons
Jingyi Xie, Jie Ju,

Ping Zhou

et al.

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Feb. 8, 2024

Histone lysine crotonylation (Kcr) is a new acylation modification first discovered in 2011, which has important biological significance for gene expression, cell development, and disease treatment. In the past over ten years, numerous signs of progress have been made research on biochemistry Kcr modification, especially series modification-related "reader", "eraser", "writer" enzyme systems are identified. The physiological function its correlation with heredity, spermatogenesis paid more attention. However, development usually associated abnormal modification. this review, we summarized identification Kcr-related system, functions, diseases caused by Kcr. This knowledge supplies theoretical basis further exploring future.

Language: Английский

Citations

10
PTMs of PD-1/PD-L1 and PROTACs application for improving cancer immunotherapy DOI Creative Commons

Xiaohui Ren,

Lijuan Wang, Likun Liu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 4, 2024

Immunotherapy has been developed, which harnesses and enhances the innate powers of immune system to fight disease, particularly cancer. PD-1 (programmed death-1) PD-L1 death ligand-1) are key components in regulation system, context cancer immunotherapy. regulated by PTMs, including phosphorylation, ubiquitination, deubiquitination, acetylation, palmitoylation glycosylation. PROTACs (Proteolysis Targeting Chimeras) a type new drug design technology. They specifically engineered molecules that target specific proteins within cell for degradation. have designed demonstrated their inhibitory activity against PD-1/PD-L1 pathway, showed ability degrade proteins. In this review, we describe how improve efficacy could be novel strategy combine with radiotherapy, chemotherapy immunotherapy patients.

Language: Английский

Citations

6
The effects of glycosylation modifications on monocyte recruitment and foam cell formation in atherosclerosis DOI Creative Commons

Da Teng,

Wenlong Wang, Wenjuan Jia

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(3), P. 167027 - 167027

Published: Jan. 17, 2024

Language: Английский

Citations

4
The role of myocardial energy metabolism perturbations in diabetic cardiomyopathy: from the perspective of novel protein post-translational modifications DOI Creative Commons
Dongze Li, Li Zhang, Qiming Gong

et al.

Clinical Epigenetics, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 26, 2025

Diabetic cardiomyopathy (DbCM), a significant chronic complication of diabetes, manifests as myocardial hypertrophy, fibrosis, and other pathological alterations that substantially impact cardiac function elevate the risk cardiovascular diseases patient mortality. Myocardial energy metabolism disturbances in DbCM, encompassing glucose, fatty acid, ketone body lactate metabolism, are crucial factors contribute to progression DbCM. In recent years, novel protein post-translational modifications (PTMs) such lactylation, β-hydroxybutyrylation, succinylation have been demonstrated be intimately associated with process, conjunction acetylation, they participate regulation activity gene expression cardiomyocytes. This review examines epigenetic pathogenesis primarily focusing on perturbations PTMs them. It provides detailed analysis mechanisms these DbCM enhance understanding pathophysiology establish theoretical foundation for development new treatment strategies

Language: Английский

Citations

0
Posttranslational modifications in cardiac metabolic remodeling mediated by metabolites: Implications for disease pathology and therapeutic potential DOI

Lifei Guo,

Yang Du, Heng Li

et al.

Metabolism, Journal Year: 2025, Volume and Issue: unknown, P. 156144 - 156144

Published: Jan. 1, 2025

Language: Английский

Citations

0
The up-regulation of ANXA1 by FTO-dependent demethylation alleviates NLRP3-mediated pyroptosis and inflammation in myocardial ischemia–reperfusion injury DOI

Chao‐Jie He,

H. Fan, Chunyan Zhu

et al.

Molecular & Cellular Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 15, 2025

Language: Английский

Citations

0
Single Cell Proteomics Reveals Novel Cell Phenotypes in Marfan Mouse Aneurysm DOI Open Access

Louis Saddic,

Giselle Kaneda, Amanda Momenzadeh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

Abstract Background Single-cell omics technology is a powerful tool in biomedical research. However, single cell proteomics has lagged due to an inability amplify peptides similar fashion nucleotide strings. Single important because proteins are the main functional unit cells, and they often poorly correlate with mRNA quantities. In this paper we describe first proteomic analysis of complex tissue, comparing aneurysmal normal mouse aorta from males females. We also compare integrate our profiles matching transcriptomics dataset. Methods compared proteomes between male female, wild-type Fbn1 C1041G/+ Marfan mice (N=3 per group). Individual cells aortic root suspensions were deposited 384 well plates subjected ultra-sensitive nanoflow liquid chromatography-ion mobility-time flight-mass spectrometry. The data analyzed leiden clustering identify types. Statistical analyses performed detect differential within types multi-omics integrated published RNA-seq. Results identified all major including 7 distinct smooth muscle subtypes. proportion these varied based on sex genotype. Differentially expressed female addition samples uncovered enhanced endothelial mesenchymal transition patterns mice. Comparisons RNA showed similarities subtypes but not Multi-omics two platforms demonstrated potential novel role for derived angiotensin signaling phenotype. Conclusions new subpopulations vascular muscles type specific protein signatures related differences aneurysm formation. Abbreviations Next generation sequencing (NGS), Mass spectrometer (MS), by Spectrometry (ScOPE-MS), Marfan’s syndrome (MFS), Fibrillin 1 (FBN1), Transforming growth factor β (TGFβ), Smooth (SMC), (scProteomic), (DEPs), Wild-type (WT), Hanks’ balanced salt solution (HBSS), Fetal bovine serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM), Data-independent acquisition parallel accumulation-serial fragmentation (DIA-PASEF), Magnetic assisted sorted (MACS), Cell Analysis Python (Scanpy), Kyoto Encyclopedia Genes Genomes (KEGG), Principal component (PCA), Uniform manifold projection (UMAP), transcriptomic (scTranscriptomic), Smoothelin (Smtn), Transgelin (Tagln), Myosin heavy chain 11 (Myh11), Platelet adhesion molecule (Pecam1), Dipeptidase (Dpep1), Uncoupling (Ucp1), Low-density lipoprotein receptor-related (Lrp1), DNA ligase 3 (Lig3), Capsaicin channel transient receptor vanilloid (Trpv1), Endothelial (endMT), Intercellular (Icam1), 2 (Icam2), cell-selective (Esam), Calponin (Cnn1), Vimentin (Vim), Zinc finger E-box-binding homeobox (Zeb1), Snail family transcriptional repressor (Snai1), Tropomyosin alpha-4 (Tpm4), Angiotensin converting enzyme (Ace)

Language: Английский

Citations

0
Regulation of autophagy: Insights into O-GlcNAc modification mechanisms DOI
Chengzhi Liu, Xinyu Wang, Shengnan Xu

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: 369, P. 123547 - 123547

Published: March 7, 2025

Language: Английский

Citations

0
The role of lactate metabolism and lactylation in pulmonary arterial hypertension DOI Creative Commons
Tao Peng,

Jian-Liang Lu,

Xiaojiao Zheng

et al.

Respiratory Research, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 12, 2025

Pulmonary arterial hypertension (PAH) is a complex and progressive disease characterized by elevated pulmonary artery pressure vascular remodeling. Recent studies have underscored the pivotal role of metabolic dysregulation epigenetic modifications in pathogenesis PAH. Lactate, byproduct glycolysis, now recognized as key molecule that links cellular metabolism with activity regulation. findings indicate that, addition to altered glycolytic dysregulated. Lactate homeostasis lactylation—a novel modification—also play significant development This review synthesizes current knowledge regarding relationship between PAH, particular focus on cumulative effects lactate cells. Furthermore, lactylation, an emerging modification, discussed context By elucidating interplay lactylation this aims provide insights into potential therapeutic targets. Understanding these pathways may lead innovative strategies for managing PAH improving patient outcomes. Future research should underlying mechanisms through which influences pathophysiology thereby aiding targeted interventions.

Language: Английский

Citations

0
Lactylation: a promising therapeutic target in ischemia-reperfusion injury management DOI Creative Commons
Feixiang Wang, Guo Mu,

Zihang Yu

et al.

Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 13, 2025

Abstract Ischemia-reperfusion injury (IRI) is a critical condition that poses significant threat to patient safety. The production of lactate increases during the process IRI, and serves as crucial indicator for assessing severity such injury. Lactylation, newly discovered post-translational modification in 2019, induced by lactic acid predominantly occurs on lysine residues histone or nonhistone proteins. Extensive studies have demonstrated pivotal role lactylation pathogenesis progression various diseases, including melanoma, myocardial infarction, hepatocellular carcinoma, Alzheimer’s disease, nonalcoholic fatty liver disease. Additionally, marked correlation between inflammation has been observed. This article provides comprehensive review mechanism underlying IRI establish theoretical foundation better understanding interplay IRI.

Language: Английский

Citations

0