Arabian Journal of Chemistry, Journal Year: 2024, Volume and Issue: 17(12), P. 106040 - 106040
Published: Nov. 2, 2024
Language: Английский
Biomaterials, Journal Year: 2024, Volume and Issue: 314, P. 122903 - 122903
Published: Oct. 23, 2024
Citations
4
Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(1)
Published: Jan. 1, 2025
ABSTRACT Osteoporosis, recognised as a metabolic disorder, has emerged significant burden on global health. Although available treatments have made considerable advancements, they remain inadequately addressed. In recent years, the role of epigenetic mechanisms in skeletal disorders garnered substantial attention, particularly concerning m 6 A RNA modification. is most prevalent dynamic and reversible modification eukaryotes, mediating various processes mRNAs, including splicing, structural conversion, translation, translocation degradation serves crucial component Research increasingly validated that plays vital proliferation, differentiation, migration, invasion,and repair bone marrow mesenchymal stem cells (BMSCs), osteoblasts osteoclasts, all which impact whole process osteoporosis pathogenesis. Continuous efforts been to target regulators natural products derived from traditional medicine, exhibit multiple biological activities such anti‐inflammatory anticancer effects, valuable resources for drug discovery. This paper elaborates methylation its regulatory osteoporosis, emphasising implications diagnosis treatment, thereby providing theoretical references.
Language: Английский
Citations
0
The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(2)
Published: Jan. 20, 2025
Abstract Abdominal aortic aneurysm represents a critical pathology of the aorta that currently lacks effective pharmacological interventions. TNF receptor‐associated factor 6 (TRAF6) has been established to be involved in cardiovascular diseases such as atherosclerosis, hypertension, and heart failure. However, its role abdominal (AAA) remains unclear. This study aimed explore TRAF6 on AAA formation underlying mechanisms. Single‐cell RNA sequencing human tissues demonstrated was significantly upregulated macrophages. Moreover, overexpression promotes elastase‐induced C57BL/6 mice, while inhibition could attenuate development. Consistently, macrophages through vitro methods notably limits their pyroptosis, also diminishing proinflammatory responses these cells. Mechanistically, can modulate macrophage pyroptosis NLRP3/Caspase1/GSDMD signaling pathway. Our highlights crucial TRAF6/NLRP3/Caspase1/GSDMD axis AAA, offering potential biomarkers therapeutic targets for AAA.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 3, 2025
Cellular aging is a multifactorial and intricately regulated physiological process with profound implications. The interaction between cellular senescence cancer complex multifaceted, can both promote inhibit tumor progression through various mechanisms. M6A methylation modification regulates the of cells tissues by modulating senescence-related genes. In this review, we comprehensively discuss characteristics senescence, signaling pathways regulating biomarkers mechanisms anti-senescence drugs. Notably, review also delves into interactions cancer, emphasizing dual role senescent microenvironment in initiation, progression, treatment. Finally, thoroughly explore function mechanism m6A revealing its critical gene expression maintaining homeostasis. conclusion, provides comprehensive perspective on molecular biological significance offers new insights for development strategies.
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142181 - 142181
Published: March 1, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2920 - 2920
Published: March 24, 2025
Epigenetic modifications play a crucial role in regulating stem cell differentiation. Among these, N6-methyladenosine (m6A) modification significantly impacts mRNA stability and translation. However, its dental differentiation remains largely unexplored. Functional assays, including ALP activity, alizarin red S staining, qPCR, Western blot, were conducted to assess odontogenic Then, an vivo dentin formation model was used validate our findings. Additionally, we employed RNA assays m6A site mutagenesis investigate the regulatory mechanism of GATA2-mediated Our results demonstrated that overexpression GATA2 promoted SCAP Moreover, studies confirmed enhances mouse models. Conversely, knockdown or mutation sites led reduced decreased is enriched 3' untranslated region (3'UTR) mRNA, expression. findings indicate contributes post-transcriptional regulation GATA2, enhancing promoting SCAP-mediated formation. This study provides new insights into epigenetic cells suggests potential molecular target for tissue regeneration.
Language: Английский
Citations
0
Tissue Engineering Part A, Journal Year: 2025, Volume and Issue: unknown
Published: March 25, 2025
Objectives: N6-methyladenosine (m6A) modification is critical in the regulation of osteoporosis (OP). Although ZC3H13 an important m6A methyltransferase, its specific regulatory effects and mechanisms are not yet fully understood. Therefore, we investigated impact on osteogenic potential bone marrow-derived mesenchymal stem cells (BMSCs) attempted to elucidate underlying mechanism. Materials Methods: Western blotting, quantitative reverse transcription polymerase chain reaction, immunohistochemical staining were used identify changes factor (RUNX2 OPN) expression osteoporosis. Gain- loss-of-function experiments conducted study differentiation osteoporotic BMSCs (OP-BMSCs). Transcriptomic sequencing, transmission electron microscopy, intraperitoneal injection ferroptosis inhibitor ferrostatin-1 (Fer-1) downstream regulated by In addition, rescue assays performed molecular involved. Results: Here, revealed that was downregulated OP-BMSCs rat femurs, which correlated with reduced OP-BMSCs. Functionally, knockdown resulted decreased BMSCs, whereas overexpression promoted Furthermore, closely related metal ion binding, cell proliferation, altered mitochondrial morphology. Treatment Fer-1 partially reversed phenotypes vivo. Mechanistically, shown promote inhibiting ferroptosis. Conclusions: Our This research offers a reliable theoretical foundation for predicting treating
Language: Английский
Citations
0
Human Cell, Journal Year: 2024, Volume and Issue: 37(4), P. 1024 - 1038
Published: April 30, 2024
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 7, 2024
Abstract This study focuses on addressing the challenges of dental implants in geriatric population by enhancing bioactivity polyetheretherketone (PEEK) through surface modification. PEEK, with its elastic modulus close to alveolar bone, mitigates stress shielding but faces limitations osseointegration due low bioactivity. We introduced zinc (Zn) and metformin (MF) onto PEEK surfaces via a dopamine-assisted physical adhesion method, creating functionalized derivative called ZnMF@PEEK. combination targets diminished osteogenic potential, persistent inflammation, cell senescence, which are common issues elderly patients. Comprehensive physicochemical characterizations confirmed successful preparation ZnMF@PEEK, vitro vivo experiments systematically evaluated biocompatibility The results indicate that ZnMF@PEEK holds promise as implant material tailored specific needs elderly, multifaceted osseointegration.
Language: Английский
Citations
0
Arabian Journal of Chemistry, Journal Year: 2024, Volume and Issue: 17(12), P. 106040 - 106040
Published: Nov. 2, 2024
Language: Английский
Citations
0