Piperazine-Substituted Pyranopyridines Exhibit Antiproliferative Activity and Act as Inhibitors of HBV Virion Production DOI Open Access
Sona Buloyan, Arpine S. Harutyunyan, H. V. Gasparyan

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 3991 - 3991

Published: April 23, 2025

Advances in medicinal chemistry have led to the development of anticancer and anti-infectious drugs. However, many types cancer viral infections such as hepatitis B virus or SARS-CoV-2 are still treated ineffectively. Therefore, further effective selective lead compounds potential drugs is highly demanded. In this study, we synthesized a novel series piperazine-substituted pyranopyridines evaluated their antiviral properties. Antiproliferative activity was determined panel various tumor cell lines well non-tumor hepatic HepaRG cells. Mechanisms cytotoxicity were assessed by fluorescent microscopy techniques. Antiviral analyzed towards DNA RNA viruses infectious culture systems. Several showed antiproliferative at micromolar submicromolar concentrations. involve induction apoptosis not mediated via ERK1/2 pathway oxidative stress. exhibit against preventing formation virion particles. This study identification class with wide range non-toxic inhibitor HBV production.

Language: Английский

PAMAM/miR-144 Nanocarrier System Inhibits the Migration of Gastric Cancer by Targeting mTOR Signal Transduction Pathway DOI
Yuanyuan Qian, Dongxu Zhu,

XU Qiong

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 249, P. 114492 - 114492

Published: Jan. 5, 2025

Language: Английский

Citations

0
Systematic Comparison of FBS and Medium Variation Effect on Key Cellular Processes Using Morphological Profiling DOI Creative Commons
Timofey Lebedev,

Alesya M. Mikheeva,

Valentina A. Gasca

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(5), P. 336 - 336

Published: Feb. 25, 2025

Although every cell biologist knows the importance of selecting right growth conditions and it is well known that composition medium may vary depending on a product brand or lot affecting many cellular processes, still those effects are poorly systematized. We addressed this issue by comparing effect 12 fetal bovine sera (FBS) eight media from different brands morphological functional parameters five types: lung adenocarcinoma, neuroblastoma, glioblastoma, embryonic kidney, colorectal cancer cells. Using high-throughput imaging, we compared proliferation; performed profiling based imaging 561,519 cells; measured extracellular regulated kinases (ERK1/2) activity, mitochondria potential, lysosome accumulation; sensitivity to drugs, response EGF stimulation, ability differentiate. found changes in proliferation morphology were independent, associated with differences potential cell's Surprisingly, most drastic detected serum-free conditions, where choice affected survival EGF. Overall, our data be used improve reproducibility experiments involving cultures, 28 44 can explored through Shinyapp.

Language: Английский

Citations

0
Piperazine-Substituted Pyranopyridines Exhibit Antiproliferative Activity and Act as Inhibitors of HBV Virion Production DOI Open Access
Sona Buloyan, Arpine S. Harutyunyan, H. V. Gasparyan

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 3991 - 3991

Published: April 23, 2025

Advances in medicinal chemistry have led to the development of anticancer and anti-infectious drugs. However, many types cancer viral infections such as hepatitis B virus or SARS-CoV-2 are still treated ineffectively. Therefore, further effective selective lead compounds potential drugs is highly demanded. In this study, we synthesized a novel series piperazine-substituted pyranopyridines evaluated their antiviral properties. Antiproliferative activity was determined panel various tumor cell lines well non-tumor hepatic HepaRG cells. Mechanisms cytotoxicity were assessed by fluorescent microscopy techniques. Antiviral analyzed towards DNA RNA viruses infectious culture systems. Several showed antiproliferative at micromolar submicromolar concentrations. involve induction apoptosis not mediated via ERK1/2 pathway oxidative stress. exhibit against preventing formation virion particles. This study identification class with wide range non-toxic inhibitor HBV production.

Language: Английский

Citations

0