Cryopreservation of Oocyte in Livestock Animals: Principles, Techniques and Updated Outcomes

Veterinary medicine and science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Many biotechnologies are currently used in livestock breeding with the aim of improving reproductive efficiency and increasing rate genetic progress production animals. The term “cryopreservation” refers to methods that allow biological samples be frozen then warmed again without losing their vitality. Cryopreservation is a process freezes stores fertilized oocytes for later use, such as gametes, embryos, primordial germ cells; it component assisted technology. While some procedures still employ slow-freezing methods, majority now use vitrification, or extremely rapid freezing, both embryos since reduces possibility harm because there not much ice crystal formation methods. Vitrification has proven useful variety applications, including vitro (IVP) agriculturally significant endangered animal species, pigs, sheep, goats, cattle, etc., after fertilization (IVF) human embryology clinics.

Language: Английский

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Nanotechnological Innovations in Healthcare

Proceedings of London International Conferences, Journal Year: 2024, Volume and Issue: 11, P. 171 - 181

Published: Oct. 15, 2024

Nanotechnology is a concept much older and more prevalent than you may think. This article will delve into the applications of nanotechnology in various fields medicine. Using ideas research, old new, this publication uses studies to explore how saves, improves, and, some cases, enables life. Frankly, discussed further paper have nothing common other significant interesting nanotechnology. However, even with diverse array fields, only fraction nanotechnology’s massive impact across medicinal practice altogether covered. has broken almost every major sector medicine, finding use from routine practices, such as drug delivery, all way extraordinary procedures, bone regeneration. opens up on cardiovascular, reproductive, antiviral, skeletal, surgical fields. A substantial amount research been conducted show that no longer limited science fiction, grow time technology. Doctors scientists are making full capabilities by using it any cases require precision effectiveness either impossible or extremely difficult dangerous when performed human hands. makes many treatments less hazardous effective, saving improving an exponential number lives goes on.

Language: Английский

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A review on the functional roles of trehalose during cryopreservation of small ruminant semen

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: Nov. 19, 2024

Sperm cryopreservation is an approach to preserve sperm cells in liquid nitrogen or other cryogenic media for future use assisted reproductive technologies, such as vitro fertilization artificial insemination. has been extensively used the dairy industry and attained excellent results after However, small ruminants application of limited, due poor quality frozen semen special characteristics female tract. In order improve post-thaw various cryoprotectants are used. Currently, many types cryoprotectants, permeable organic solvents, sugars, antioxidants, natural synthetic ice blockers, have tested on ruminants’ cryopreservation. Among them, trehalose; shown potential acting cryoprotectant freezing. While, exact roles action mechanisms trehalose during remain unclear. this review, we systematically summarized present usage status, mechanisms, prospects small-ruminant

Language: Английский

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Proteomic analysis reveals the sperm protein difference among fresh and frozen semen of Niangya yak in Xizang

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Background To enhance Niangya yak's reproductive efficiency and breeding process, this study employed Label-free quantitative proteomics technology to analyze fresh (F) chilled (C) semen samples.Result The analysis revealed an extensive repertoire of 18,338 peptide segments 2,579 proteins within yak semen. Among them, 2,300 were successfully annotated in the database. Notably, a total 300 DEPs identified between C F groups; comprising 129 up-regulated 171 down-regulated DEPs. GO enrichment highlighted that these may be involved lipid metabolism as well protein binding with proteases phosphorylation processes associated sperm cryodamage. KEGG showed 35 mapped pathways related motility, plasma membrane integrity apoptosis. Specifically, ribosome pathway, glycerophospholipid ferroptosis phospholipase D signaling pathway might closely linked decreased vitality, damaged integrity, apoptosis induced by freezing Based on PPI groups F, has preliminarily potential HSPBP1, HSP90AB1, CCDC83, PMSD4, ISYNA1, KLHL10, UAB1 are vitality cell caused cryodamage.Conclusion This represents first their involvement biological frozen using technology. It provides theoretical foundation for comprehending mechanism cryodamage enhancing yaks.

Language: Английский

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Proteomic analysis to explore potential mechanism underlying pseudomale sperm defect in Cynoglossus semilaevis

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 40, P. 102553 - 102553

Published: Dec. 7, 2024

Language: Английский

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