Overexpression of IL-10 in Adipose Mesenchymal Stem Cells Promotes Wound Healing in Diabetic Mice

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Background: Diabetic ulcer is a serious chronic non-healing wound, which often leads to amputation or even death, causing great damage the patients and their families. In recent years, stem cells are increasingly studied for tissue repair. Adipose tissue-derived mesenchymal (Adipose-derived cells, ADSCs) have advantages of wide source easy access. ADSCs can accelerate wound healing reduce scar hyperplasia, especially wounds, cannot heal with traditional treatments, bring hope these patients. Our study intends overexpress IL-L 10 in by genetic engineering technology, transplant ADSC-IL diabetic mouse use role IL-10 promoting M2 macrophages transformation, combined achieve rapid wounds. Methods: The expression protein supernatant ADSC-IL10 was assessed using an ELISA kit. Flow cytometry employed analyze surface cell markers (CD44, CD73, CD90, CD105) ADSC-IL10. Additionally, migration proliferation were evaluated through scratch assays MTT assays. lipogenic marker PPARγ osteogenic RUNX2 detected fluorescent real-time quantitative PCR. Conditioned media from (ADSC-IL10-CM) ADSC-PCDH (ADSC-CM) culture peritoneal (Raw 264.7). Following 12-hour incubation period, Raw 264.7 harvested mRNA extraction. types identified qPCR, M1 phenotype determined CD86 Arg-1 CD206. levels inflammatory factors, including IL-1β, IL-6, IL-10, MCP-1, growth factors such as EGF, VEGF, TGFβ-1, quantified qPCR. transwell method utilized assess impact ADSC-IL10-CM ADSC-CM on normal skin fibroblasts. scribing applied examine effect human immortalized epidermal cells. A model induced high-fat, high-sugar diet streptozocin. group, 1×10^6 transplanted onto 1.5cm×1.5cm surface, similarly, group. control group received equal volume 0.9% saline. process mice observed documented at various time points. Tissue samples wounds days 3 7 subjected histological staining qPCR analysis. HE used monitor progress, while immunofluorescence (CD206, Arg-1) quantify presence M2-type skin. CD206 genes measured ascertain macrophage phenotypes within tissues. Levels MCP-1 evaluate status TGFβ-1 tissues assessed. Conclusion: overexpression does not alter biological characteristics ADSCs. When into mice, more effectively than alone. mechanism enhances may encompass several aspects: it stimulates macrophages, suppresses secretion pro-inflammatory promotes production like VEGF. encourages fibroblasts sites mice.

Language: Английский

---

A Biomimetic Nanomedicine Alleviates Liver Transplant-Related Biliary Injury by Sequentially Inhibiting Oxidative Stress and Regulating Macrophage Polarization via Nrf-2/HO-1 and JNK Pathways

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101797 - 101797

Published: April 1, 2025

Language: Английский

---

Intelligent hydrogel-based dressings for treatment of chronic diabetic wounds

World Journal of Diabetes, Journal Year: 2025, Volume and Issue: 16(5)

Published: April 24, 2025

Diabetic wounds represent a significant challenge in the medical field, significantly impacting patient quality of life and imposing heavy burden on healthcare systems. Intelligent hydrogel dressings have attracted attention diabetic wound treatment due to their unique properties. This review systematically explores three main categories intelligent hydrogels (natural, synthetic, composite), dissecting composition, structure, mechanisms that enable responses. The crucial roles these maintaining moist environment, efficiently absorbing exudate, precisely delivering drugs are expounded. Moreover, application advantages combating bacteria infections, regulating inflammation immunity, promoting angiogenesis tissue regeneration, as well enabling real-time monitoring personalized treatment, explored depth. Additionally, we discuss future research directions prospects for precision medicine care, aiming inspire innovation provide comprehensive theoretical basis development next-generation dressings.

Language: Английский

---

Discovery of sinomenine derivatives inhibiting macrophage polarization against rheumatoid arthritis through selectively targeting heme oxygenase-1

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 117596 - 117596

Published: April 1, 2025

Language: Английский

---