Evidence of Silencing of Intestinal Inflammatory and Immune Transcripts Following Induction of Joint Inflammation DOI Open Access
Meghan M. Moran, Jun Li, Quan Shen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

ABSTRACT Disrupted intestinal epithelial barrier function has been proposed to be integral rheumatoid arthritis (RA) progression and pathogenesis. To further define the molecular pathways in synovial inflammation response of tissues, we have used a rat model mono-joint inflammatory arthritis, induced by intra-articular injection Complete Freund’s adjuvant (CFA). The predominant single into knee joint resulted rapid reproducible formation fibrotic myeloid-infiltrated pannus. Our aim was determine how including proximal distal ileum colon, responded changes synovium temporally coordinated manner comparing their transcriptomic landscapes using RNASeq analyses. We confirmed timeline swelling measurement, increased fluid levels bikunin (a component both acute phase protein pre-alpha-inhibitor inter-alpha-inhibitor) demonstrated self-correcting trabecular cortical bone CFA challenge. Intestine-specific responses were monitored 16S microbiome amplicon sequencing, histopathology for mucus layer integrity, immune cell immunohistochemistry. present data that shows tissue displays an allostatic region specific. primarily with secretion silencing T-cell specific pathways, whereas colon showed transient upregulation macrophages, broader suppression related metabolic pathway transcripts. Interestingly, many neuropathways activated early but then suppressed later colon. There only insignificant fecal composition or post-CFA administration. In summary, our show first time following induction minimal microbiome. results help clarify tissues stresses accompany pathogenesis diseases.

Language: Английский

Evidence of Silencing of Intestinal Inflammatory and Immune Transcripts Following Induction of Joint Inflammation DOI Open Access
Meghan M. Moran, Jun Li, Quan Shen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

ABSTRACT Disrupted intestinal epithelial barrier function has been proposed to be integral rheumatoid arthritis (RA) progression and pathogenesis. To further define the molecular pathways in synovial inflammation response of tissues, we have used a rat model mono-joint inflammatory arthritis, induced by intra-articular injection Complete Freund’s adjuvant (CFA). The predominant single into knee joint resulted rapid reproducible formation fibrotic myeloid-infiltrated pannus. Our aim was determine how including proximal distal ileum colon, responded changes synovium temporally coordinated manner comparing their transcriptomic landscapes using RNASeq analyses. We confirmed timeline swelling measurement, increased fluid levels bikunin (a component both acute phase protein pre-alpha-inhibitor inter-alpha-inhibitor) demonstrated self-correcting trabecular cortical bone CFA challenge. Intestine-specific responses were monitored 16S microbiome amplicon sequencing, histopathology for mucus layer integrity, immune cell immunohistochemistry. present data that shows tissue displays an allostatic region specific. primarily with secretion silencing T-cell specific pathways, whereas colon showed transient upregulation macrophages, broader suppression related metabolic pathway transcripts. Interestingly, many neuropathways activated early but then suppressed later colon. There only insignificant fecal composition or post-CFA administration. In summary, our show first time following induction minimal microbiome. results help clarify tissues stresses accompany pathogenesis diseases.

Language: Английский

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