Tracking inflammation resolution signatures in lungs after SARS-CoV-2 omicron BA.1 infection of K18-hACE2 mice DOI Creative Commons
Agnes Carolin, Kexin Yan, Cameron Bishop

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0302344 - e0302344

Published: Nov. 12, 2024

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes Coronavirus Disease 2019 (COVID-19), which can result in disease, often characterised by a 'cytokine storm' and the associated distress syndrome. However, many infections with SARS-CoV-2 are mild or asymptomatic throughout course of infection. Although blood biomarkers disease well studied, less understood inflammatory signatures lung tissues silent infections, wherein infection inflammation rapidly resolved leading to sequelae-free recovery. Herein we described RNA-Seq histological analyses lungs over time an omicron BA.1/K18-hACE2 mouse model, displays these latter features. robust was evident at days post (dpi), viral RNA largely cleared 10 dpi. Acute showed slightly different pattern cytokine compared models, where much diminished 30 dpi absent 66 Cellular deconvolution identified significantly increased abundance scores for number anti-inflammatory pro-resolution cell types 5/10 These included type II innate lymphoid cells, T regulatory interstitial macrophages. Genes whose expression trended downwards 2-66 were pathways. upward during this period recovery ciliated AT2 AT1 transition, reticular fibroblasts indicating return homeostasis. Very few differentially expressed host genes dpi, suggesting near complete parallels between subclinical humans those observed model discussed reference concept "protective inflammation".

Language: Английский

Cross-Reactivity Assessment of Vaccine-Derived SARS-CoV-2 T Cell Responses against BA.2.86 and JN.1 DOI Creative Commons
Muhammad S. Sohail, Syed Faraz Ahmed, Ahmed Abdul Quadeer

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(3), P. 473 - 473

Published: March 20, 2024

The SARS-CoV-2 Omicron sub-variants BA.2.86 and JN.1 contain multiple mutations in the spike protein that were not present previous variants of concern sub-variants. Preliminary research suggests these reduce neutralizing capability antibodies induced by vaccines, which is particularly significant for JN.1. This raises as many widely deployed COVID-19 vaccines are based on ancestral Wuhan strain SARS-CoV-2. While T cell responses have been shown to be robust against variants, less known about impact responses. We evaluate effect specific experimentally determined epitopes derived from XBB.1.5 has recommended a booster vaccine. Our data suggest affect numerous compared variants; however, widespread loss recognition unlikely.

Language: Английский

Citations

12
Evolution of SARS-CoV-2 T cell responses as a function of multiple COVID-19 boosters DOI Creative Commons
Ricardo da Silva Antunes,

Vicente Fajardo-Rosas,

Esther Dawen Yu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

SUMMARY The long-term effects of repeated COVID-19 vaccinations on adaptive immunity remain incompletely understood. Here, we conducted a comprehensive three-year longitudinal study examining T cell and antibody responses in 78 vaccinated individuals without reported symptomatic infections. We observed distinct dynamics Spike-specific humoral cellular immune across multiple vaccine doses. While titers incrementally increased stabilized with each booster, rapidly plateaued, maintaining remarkable stability CD4+ CD8+ subsets. Notably, approximately 30% participants showed reactivity to non-Spike antigens, consistent asymptomatic Single-cell RNA sequencing revealed diverse landscape phenotypes, no evidence exhaustion or significant functional impairment. However, qualitative changes were infection, exhibiting unique immunological characteristics, including frequencies Th17-like cells GZMKhi/IFNR Remarkably, this group associated progressive increase regulatory cells, potentially indicating balanced response that may mitigate immunopathology. By regularly stimulating memory, boosters contribute stable enhanced response, which provide better protection against

Language: Английский

Citations

1
Discordant Outcomes of SARS-CoV-2 Infection in Household Contacts DOI
Ye‐Ji Lee, Alison Tarke, Tertuliano Alves Pereira Neto

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Detection of SARS-CoV-2 Reinfections Using Nucleocapsid Antibody Boosting DOI
Eduard Grebe,

Daniel Chacreton,

Mars Stone

et al.

Emerging infectious diseases, Journal Year: 2025, Volume and Issue: 31(5)

Published: April 24, 2025

More than 85% of US adults had been infected with SARS-CoV-2 by the end 2023. Continued serosurveillance transmission and assessments correlates protection require robust detection reinfections. We developed a serologic method for identifying reinfections in longitudinal blood donor data assessing nucleocapsid (N) antibody boosting using total immunoglobulin assay. Receiver operating characteristic curve analysis yielded an optimal ratio >1.43 (sensitivity 87.1%, specificity 96.0%). When prioritizing specificity, >2.33 was 75.3%, 99.3%). In donors higher anti-N reactivity levels before reinfection, sensitivity reduced. Sensitivity could be improved expanding dynamic range assay through dilutional testing, from 38.8% to 66.7% highest group (signal-to-cutoff reinfection >150). This study demonstrated that testing N antibodies can used identify estimate infection incidence cohort.

Language: Английский

Citations

0
Tracking inflammation resolution signatures in lungs after SARS-CoV-2 omicron BA.1 infection of K18-hACE2 mice DOI Creative Commons
Agnes Carolin, Kexin Yan, Cameron Bishop

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 13, 2024

Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes Coronavirus Disease 2019 (COVID-19), which can result in disease, often characterised by a ‘cytokine storm’ and the associated distress syndrome. However, many infections with SARS-CoV-2 are mild or asymptomatic throughout course of infection. Although blood biomarkers disease well studied, less understood inflammatory signatures lung tissues silent infections, wherein infection inflammation rapidly resolved leading to sequelae-free recovery. Herein we described RNA-Seq histological analyses lungs over time an omicron BA.1/K18-hACE2 mouse model, displays these latter features. robust was evident at days post (dpi), viral RNA largely cleared 10 dpi. Acute showed slightly different pattern cytokine compared models, where much diminished 30 dpi absent 66 Cellular deconvolution identified significantly increased abundance scores for number anti-inflammatory pro-resolution cell types 5/10 These included type II innate lymphoid cells, T regulatory interstitial macrophages. Genes whose expression trended downwards – were pathways. upward during this period recovery ciliated AT2 AT1 transition, reticular fibroblasts indicating return homeostasis. Very few differentially expressed host genes dpi, suggesting near complete parallels between subclinical humans those observed model discussed reference concept “protective inflammation”.

Language: Английский

Citations

3
SARS-CoV-2 immunity DOI Open Access
Antonio Bertoletti

Cellular and Molecular Immunology, Journal Year: 2024, Volume and Issue: 21(2), P. 101 - 102

Published: Jan. 18, 2024

Language: Английский

Citations

1
Factors Affecting Adherence to Social Distancing among Adults Aged 19–44 Years: Insights from a Nationwide Survey during COVID-19 Pandemic DOI Creative Commons
Eun-Jung Kim, Mikyong Byun

Medicina, Journal Year: 2024, Volume and Issue: 60(5), P. 827 - 827

Published: May 17, 2024

Background and Objectives: Before COVID-19 vaccinations became available, adhering to non-pharmaceutical interventions (NPIs), like social distancing (SD), wearing masks, hand hygiene, were crucial mitigating viral spread. Many studies reported that younger individuals more reluctant follow these measures compared with older ones. We hypothesized it would be worthwhile find factors influenced SD compliance among young people during the pre-vaccination phase of a pandemic. Materials Methods: analyzed data adults aged 19–44 from 2020 South Korean Community Health Survey socio-demographic, health-related behavioral, psychological between compliant non-compliant cohorts. Results: A total 59,943 participants enrolled we found age groups (30–39 40–44) safety concerns (such as infection, virus-related death, economic damage, transmitting virus vulnerable people) significantly associated adherence SD. Conversely, who not living spouse, unable stay at home despite symptoms, smoked, drank, had negative attitude toward government policy statistically correlated non-compliance. Conclusions: In times when NPIs primary defense against pandemic, is essential identify positively or negatively affect individual them, especially people. Using large-scale, well-designed national survey, could gain insights into early recognition risk for non-compliance appropriate follow-up (i.e., education campaigns, clear communication public guidelines, implementation guidelines), which will help avoid suffering other waves future infectious diseases.

Language: Английский

Citations

0
Bioinformatics and molecular biology tools for diagnosis, prevention, treatment and prognosis of COVID-19 DOI Creative Commons
Débora Dummer Meira, Aléxia Stefani Siqueira Zetum, Matheus Correia Casotti

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34393 - e34393

Published: July 1, 2024

Since December 2019, a new form of Severe Acute Respiratory Syndrome (SARS) has emerged worldwide, caused by SARS coronavirus 2 (SARS-CoV-2). This disease was called COVID-19 and declared pandemic the World Health Organization in March 2020. Symptoms can vary from common cold to severe pneumonia, hypoxemia, respiratory distress, death. During this period world stress, medical scientific community were able acquire information generate data at unprecedented speed, better understand facilitate vaccines therapeutics development. Notably, bioinformatics tools instrumental decoding viral genome identifying critical targets for diagnosis therapeutics. Through integration omics data, also improved our understanding pathogenesis virus-host interactions, facilitating development targeted treatments vaccines. Furthermore, molecular biology techniques have accelerated design sensitive diagnostic tests characterization immune responses, paving way precision medicine approaches treating COVID-19. Our analysis highlights indispensable contributions global effort against In review, we aim revise features, diagnostic, prevention, treatment options, how biology, modern bioinformatic tools, collaborations helped combat pandemic. An integrative literature review performed, searching articles on several sites, including PUBMED Google Scholar indexed referenced databases, prioritizing last 3 years. The lessons learned will place much position respond future pandemics.

Language: Английский

Citations

0
Tracking inflammation resolution signatures in lungs after SARS-CoV-2 omicron BA.1 infection of K18-hACE2 mice DOI Creative Commons
Agnes Carolin, Kexin Yan, Cameron Bishop

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0302344 - e0302344

Published: Nov. 12, 2024

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes Coronavirus Disease 2019 (COVID-19), which can result in disease, often characterised by a 'cytokine storm' and the associated distress syndrome. However, many infections with SARS-CoV-2 are mild or asymptomatic throughout course of infection. Although blood biomarkers disease well studied, less understood inflammatory signatures lung tissues silent infections, wherein infection inflammation rapidly resolved leading to sequelae-free recovery. Herein we described RNA-Seq histological analyses lungs over time an omicron BA.1/K18-hACE2 mouse model, displays these latter features. robust was evident at days post (dpi), viral RNA largely cleared 10 dpi. Acute showed slightly different pattern cytokine compared models, where much diminished 30 dpi absent 66 Cellular deconvolution identified significantly increased abundance scores for number anti-inflammatory pro-resolution cell types 5/10 These included type II innate lymphoid cells, T regulatory interstitial macrophages. Genes whose expression trended downwards 2-66 were pathways. upward during this period recovery ciliated AT2 AT1 transition, reticular fibroblasts indicating return homeostasis. Very few differentially expressed host genes dpi, suggesting near complete parallels between subclinical humans those observed model discussed reference concept "protective inflammation".

Language: Английский

Citations

0