Cell chemical biology, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)
Published: Jan. 13, 2025
Colorectal cancer (CRC) is a prevalent malignancy worldwide, associated with significant morbidity and mortality. Cyclin-dependent kinase 1 (CDK1) plays crucial role in cell cycle regulation has been implicated various cancers. This study aimed to evaluate the prognostic value of CDK1 CRC identify traditional Chinese medicines (TCM) that can target as potential treatments for CRC. The expression were analyzed through TCGA, GEO, GEPIA, UALCAN HPA databases. An ESTIMATE analysis was applied estimate proportions stromal immune cells tumor samples. GO KEGG enrichment analyses performed clarify functional roles CDK1-related genes. CCK-8, colony formation, migration, invasion, wound healing assays employed explore tumor-promoting CDK1. Molecular docking, cellular thermal shift, isothermal dose-response inhibitors highly expressed poorer prognosis. also correlated levels infiltration. genes primarily involved P53 signaling pathway. Knockdown inhibited proliferation, invasion vitro. Furthermore, Eriocitrin emerged inhibitor, exerting its anti-tumor effects by targeting inhibiting activity. critical Eriocitrin, inhibitor derived from TCM, highlights promising new therapeutic strategy treatment.
Language: Английский
Citations
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Liver International, Journal Year: 2025, Volume and Issue: 45(4)
Published: March 7, 2025
Transmembrane (TMEM) proteins are involved in fundamental biological processes such as material transport and signal transduction. TMEM115 is a member of the TMEM protein family, but its significance hepatocellular carcinoma (HCC) remains unclear. In this study, we investigate clinical predictive potential functions HCC. Bioinformatics was used to mRNA expression immune infiltration score. Through multiplex immunohistochemistry analysis, assessed association with HCC patient features, prognosis cell vitro vivo experiments, evaluated cells impact on microenvironment. levels were significantly higher tissues compared paracancerous liver tissues. Its correlated characteristics overall survival patients. tissues, corresponded lower proportions CD66b+ neutrophils CD8+ T proportion CD4+ cells. Furthermore, patients low displayed programmed death ligand-1 lymphocyte activation gene 3 expression. Functionally, knockdown inhibited proliferation, migration invasion orthotopic models, growth affected Our findings show promising prognostic indicator for hold promise predicting responses therapy, emphasising relevance intricate involvement microenvironment
Language: Английский
Citations
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Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 15, 2025
Multimodal phototheranostics utilizing single molecules offer a "one-and-done" approach, presenting convenient and effective strategy for cancer therapy. However, therapies based on conventional photosensitizers often suffer from limitations such as photosensitizing mechanism, restricted tumor penetration retention, the requirement multiple irradiations, which significantly constrain their application. In this report, we present an aggregation-induced emission luminogen (AIEgen) bacteria hybrid bionic robot to address above issues. This is composed of multifunctional AIEgen (INX-2) Escherichia coli Nissle 1917 (EcN), i.e., EcN@INX-2. The EcN@INX-2 exhibits near-infrared II (NIR-II) fluorescence demonstrates efficient photodynamic photothermal effects, well tumor-targeting capabilities. These features are facilitated by complementary roles INX-2 EcN. successfully enables in vivo multimodal imaging therapy colon models female mice through various mechanisms, including activation anti-tumor immunity, Our study paves avenue designing diagnostic agents targeted image-guided combinational immunotherapy. utility hindered need limited retention. Here, authors these issues developing that activates immunity.
Language: Английский
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Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15
Published: March 17, 2025
Inflammation, a hallmark of cancer, has been associated with tumor progression, transition into malignant phenotype and efficacy anticancer treatments in cancer. It affects all stages from the initiation carcinogenesis to metastasis. Chronic inflammation induces immunosup-pression, providing an environment conducive carcinogenesis, whereas acute antitumor immune response, leading suppression. Solid tumors have inflammatory microenvironment (TME) containing cancer cells, stromal soluble molecules, which plays key role progression therapy response. Both cells TME are highly plastic constantly change their phenotypic functional properties. Cancer-associated inflammation, majority consists innate important cell plasticity, development drug resistance. Today, combined used advanced technologies, such as single-cell RNA sequencing spatial molecular imaging analysis, pathways linking chronic largely elucidated. In this review article, we highlighted cellular mechanisms involved cancer-associated its effects on treatment We also comprehensively setting GI cancers.
Language: Английский
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Medical Sciences, Journal Year: 2025, Volume and Issue: 13(2), P. 34 - 34
Published: April 1, 2025
Background: Among rheumatologic diseases following therapy with immune checkpoint inhibitors (ICIs), the cases of cancer patients diagnosed as having polymyalgia rheumatica (PMR), particularly nivolumab and pembrolizumab, has been steadily rising in published reports. Objectives: We performed a systematic review case reports aim answering these questions: (1) Is PMR pembrolizumab an adverse drug reaction (ADR)? (2) there difference between those pembrolizumab? Methods: Based on Preferred Reporting Items for Systematic Reviews Meta-Analysis (PRISMA) guidelines, comprehensive literature search three main bibliographic databases: MEDLINE (Ovid interface), EMBASE, COCHRANE Library was carried out 27 December 2024. This no registration number. Results: Data were extracted from 12 patients. Namely, 5 followed treatment 7 pembrolizumab. Validated scales ADR assessment—such Naranjo’s scale—were not used 10 Additionally, validated diagnostic or classification criteria majority related to nivolumab. On contrary, clinical judgment alone rule almost all Finally, time interval manifestations nivolumab/pembrolizumab ranged one 14 cycles (fully compatible pharmacokinetics). Conclusions: Our highlighted significant methodological blurred lines categorization
Language: Английский
Citations
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Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16
Published: April 7, 2025
The role of immunogenic cell death (ICD) in cervical cancer (CESC) is not well understood. This study sought to investigate the significance ICD CESC and establish an ICDRs prognostic model improve immunotherapy efficacy for patients with cancer. ICD-associated genes were screened at single-cell transcriptome levels based on AddModuleScore, single-sample gene set enrichment analysis (ssGSEA) weighted co-expression network (WGCNA) analysis. Immunogenic death-related features (ICDRs) constructed using multiple machine algorithms, evaluated training validation sets provide quantitative tools predicting prognosis clinical practice. Predictive models used risk subgroups response immunotherapy, as drug sensitivity. Finally, expression ICD-related was verified by RT-qPCR. Through integrated data, transcriptomic profiling, computational modeling, seven identified highly patients. Multivariate demonstrated that low-risk had significantly better overall survival compared high-risk patients, confirming independent tool. Assessments tumor microenvironment (TME), mutation characteristics, sensitivity within indicated a stronger group.
Language: Английский
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Small, Journal Year: 2025, Volume and Issue: unknown
Published: April 14, 2025
Abstract Immune checkpoint inhibitors (ICIs) have limited clinical efficacy against gastric cancer (GC) due to the nonimmunogenic tumor microenvironment. Therefore, inducing immunogenic cell death (ICD) reprogram landscape is essential. This study develops HD‐FA nanoparticles by encapsulating a novel mitochondrion‐targeted NIR‐II modulator, HD, within DSPE‐PEG‐FA. exhibits superior spatiotemporal resolution, robust accumulation, and minimal adverse effects. Upon 808 nm laser irradiation, generates reactive oxygen species, leading ferroptosis oxidative stress damage in GC cells inhibiting SLC7A11/GSH/GPX4 axis. triggers ICD, resulting antitumor activity not only primary tumors but also distant tumors. Moreover, promotes dendritic maturation, increases effector‐memory T‐cell frequency, reduces presence of myeloid‐derived suppressor cells, thereby fostering enhanced immunity. presents first report modulator for synergistic therapy with ICIs, marking significant advancements fight GC.
Language: Английский
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Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: April 15, 2025
Language: Английский
Citations
0
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: April 24, 2025
Clinical data have shown that Serine/Threonine Kinase 11 (STK11) mutation may be associated with an immunosuppressive tumor microenvironment (ITEM) and poor prognosis failure of anti-PD-1 (αPD1) treatment in non-small cell lung cancer (NSCLC). To explore the potential restoring STK11 protein immunotherapy, a bionic gene delivery system was prepared by coating STK11-encoded DNA-cationic polymer complex core membrane, termed STK11@PPCM. STK11@PPCM could specifically bind NSCLC cells achieve precise DNA. The released DNA effectively restored expression, consequently reactivating autophagy immunogenic death (ICD) cells. liberated damage-associated molecular patterns (DAMPs) autophagosome induced dendritic (DCs) maturation, which turn enhanced CD8 + T infiltration, M1 macrophage polarization, proinflammatory factor thereby reversing ITEM. Moreover, also found to improve sensitivity αPD1 increasing expression PD-L1, confirmed STK11-mutated xenografted mouse models, constructed CRISPR-Cas9 knockout technology. This work demonstrated for first time restoration functional can reverse ITME boost efficacy NSCLC, offering new therapeutic approach adenocarcinoma clinic.
Language: Английский
Citations
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Cell chemical biology, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
Citations
0