Nuclear and mitochondrial population genetics of the Australasian arbovirus vector Culex annulirostris (Skuse) reveals strong geographic structure and cryptic species DOI Creative Commons

William Atherton,

Luke Ambrose,

James Wisdom

et al.

Parasites & Vectors, Journal Year: 2024, Volume and Issue: 17(1)

Published: Dec. 4, 2024

Abstract Background The mosquito Culex annulirostris Skuse (Diptera: Culicidae) is an important arbovirus vector in Australasia. It part of the sitiens subgroup that also includes Cx. palpalis and . Single locus mitochondrial nuclear DNA sequencing studies suggest consists a complex at least two species. We tested this hypothesis by analysing both microsatellite data additional sequence to describe population genetics through Australia, Papua New Guinea (PNG) Solomon Archipelago. Methods Twelve novel markers for were developed used on over 500 individuals identified as molecular diagnostics. Ten 12 microsatellites then analysis using Discriminant Analysis Principal Components, Bayesian clustering software, STRUCTURE, along with estimates Jost’s D statistic similar F ST but better suited data. Mitochondrial cytochrome oxidase I (COI) generated complementing previous work analysed diversity (Haplotype diversity, Hd Pi, π), Tadjima’s D, pairwise between populations. An allele specific diagnostic internal control was developed. Results confirm existence multiple genetically geographically restricted Within mainland our findings show distinct One extends northern Australia into south-east coast Queensland South Wales (NSW). second Australian occurs inland NSW, Victoria, extending west southern Western Australia. These populations evidence possible admixture central far north Queensland. Australia’s Great Dividing Range runs down southeast presents strong gene-flow barrier these which may be driven climate, elevation or river basins. In PNG we find reproductive isolation sympatric cryptic species occurring Cape York Peninsula. A PCR-based distinguish Conclusion This study adds growing body suggesting taxon presently known now appears consist co-occur can distinguished ITS1 PCR diagnostic. Islands represent light its geographic lack sympatry other would require further study. Additionally, structure regions within latitudinal elevational subspecies hybridise where they overlap. Graphical

Language: Английский

Nuclear and mitochondrial population genetics of the Australasian arbovirus vector Culex annulirostris (Skuse) reveals strong geographic structure and cryptic species DOI Creative Commons

William Atherton,

Luke Ambrose,

James Wisdom

et al.

Parasites & Vectors, Journal Year: 2024, Volume and Issue: 17(1)

Published: Dec. 4, 2024

Abstract Background The mosquito Culex annulirostris Skuse (Diptera: Culicidae) is an important arbovirus vector in Australasia. It part of the sitiens subgroup that also includes Cx. palpalis and . Single locus mitochondrial nuclear DNA sequencing studies suggest consists a complex at least two species. We tested this hypothesis by analysing both microsatellite data additional sequence to describe population genetics through Australia, Papua New Guinea (PNG) Solomon Archipelago. Methods Twelve novel markers for were developed used on over 500 individuals identified as molecular diagnostics. Ten 12 microsatellites then analysis using Discriminant Analysis Principal Components, Bayesian clustering software, STRUCTURE, along with estimates Jost’s D statistic similar F ST but better suited data. Mitochondrial cytochrome oxidase I (COI) generated complementing previous work analysed diversity (Haplotype diversity, Hd Pi, π), Tadjima’s D, pairwise between populations. An allele specific diagnostic internal control was developed. Results confirm existence multiple genetically geographically restricted Within mainland our findings show distinct One extends northern Australia into south-east coast Queensland South Wales (NSW). second Australian occurs inland NSW, Victoria, extending west southern Western Australia. These populations evidence possible admixture central far north Queensland. Australia’s Great Dividing Range runs down southeast presents strong gene-flow barrier these which may be driven climate, elevation or river basins. In PNG we find reproductive isolation sympatric cryptic species occurring Cape York Peninsula. A PCR-based distinguish Conclusion This study adds growing body suggesting taxon presently known now appears consist co-occur can distinguished ITS1 PCR diagnostic. Islands represent light its geographic lack sympatry other would require further study. Additionally, structure regions within latitudinal elevational subspecies hybridise where they overlap. Graphical

Language: Английский

Citations

0