Transcriptomic and metabolomic profiling of flavonoid biosynthesis provides novel insights into petals coloration in Asian cotton (Gossypium arboreum L.) DOI Creative Commons

Aishuang Xing,

Xiaoyang Wang, Mian Faisal Nazir

et al.

BMC Plant Biology, Journal Year: 2022, Volume and Issue: 22(1)

Published: Aug. 30, 2022

Abstract Background Asian cotton ( Gossypium arboreum L.), as a precious germplasm resource of with insect resistance and stress tolerance, possesses broad spectrum phenotypic variation related to pigmentation. Flower color affects pollination the ornamental value plants. Studying flower varieties improves rate hybridization thus enriches diversity resources. Meanwhile, it also impacts development horticultural industry. Unfortunately, there is clear lack studies concerning intricate mechanisms flower-color differentiation. Hereby, we report an integrative approach utilizing transcriptome metabolome in three cultivars. Results A total 215 differentially accumulated metabolites (DAMs) were identified, including 83 flavonoids (DAFs). Colorless kaempferol was more abundant white flowers, while gossypetin-fer showed specificity flowers. Quercetin gossypetin main contributors yellow petal formation. Pelargonidin 3-O-beta-D-glucoside cyanidin-3-O-(6''-Malonylglucoside) high accumulation levels purple petals. pigments promoted coloration. Moreover, 8178 expressed genes (DEGs) identified by RNA sequencing. The correlation results between anthocyanins DEGs explored, indicating that 10 key structural 29 transcription factors anthocyanin biosynthesis could be candidates for accumulation. Ultimately, constructed co-expression networks DAFs demonstrated interactions specific transcripts different Conclusion This study provides new insights into elucidating regulatory lays potential foundation generate highly attractive flowers pollinators.

Language: Английский

Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes DOI Creative Commons
Nam Phuong Kieu, Marit Lenman, Eu Sheng Wang

et al.

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Feb. 24, 2021

The use of pathogen-resistant cultivars is expected to increase yield and decrease fungicide in agriculture. However, potato breeding, increased resistance obtained via genes (R-genes) hampered because R-gene(s) are often specific for a pathogen race can be quickly overcome by the evolution pathogen. In parallel, susceptibility (S-genes) important pathogenesis, loss S-gene function confers several plants, such as rice, wheat, citrus tomatoes. this article, we present mutation screening seven putative S-genes potatoes, including two DMR6 homologues. Using CRISPR/Cas9 system, which conferred co-expression guide RNAs, tetra-allelic deletion mutants were generated against late blight was assayed plants. Functional knockouts StDND1, StCHL1, DMG400000582 (StDMR6-1) potatoes with blight. Plants mutated StDND1 showed pleiotropic effects, whereas StDMR6-1 StCHL1 plants did not exhibit any growth phenotype, making them good candidates further agricultural studies. Additionally, that DMG401026923 (here denoted StDMR6-2) knockout demonstrate resistance, but exhibited indicating StDMR6-2 have different functions. To best our knowledge, first report on

Language: Английский

Citations

181
PyWRKY26 and PybHLH3 cotargeted the PyMYB114 promoter to regulate anthocyanin biosynthesis and transport in red-skinned pears DOI Creative Commons
Chuang Li, Wu Jun, Kang‐Di Hu

et al.

Horticulture Research, Journal Year: 2020, Volume and Issue: 7(1)

Published: March 15, 2020

Red pear is favored because of its bright appearance and abundant anthocyanins. Anthocyanin biosynthesis controlled by transcription factors (TFs) forming regulatory complexes. In red-skinned pears, the WRKY TFs have a significant relationship with anthocyanin biosynthesis, but molecular mechanism involved in regulating color formation unclear. this study, PyWRKY31 PyWRKY26 were screened as candidate genes for controlling transcriptome data bioinformatics analysis. The effect accumulations after cotransformation or partners PyMYB10, PyMYB114, PybHLH3 was verified tobacco leaves strawberry receptacles transient expression system. RT-qPCR analysis dual-luciferase reporter system further confirmed that activated PyDFR, PyANS, PyUFGT PyGST transport instead PyABC transporter PyAVP. Furthermore, cotransformed could bind to PyMYB114 promoter, directly PyMYB114. addition, TF interact PybHLH3, firefly luciferase complementation yeast two-hybrid (Y2H) assays. These results showed interaction cotarget which resulted accumulation pear. This study strengthened understanding contributed improving pears.

Language: Английский

Citations

161
Applications of Sesquiterpene Lactones: A Review of Some Potential Success Cases DOI Creative Commons
Laila Moujir, Oliver Callies,

Pedro M. C. Sousa

et al.

Applied Sciences, Journal Year: 2020, Volume and Issue: 10(9), P. 3001 - 3001

Published: April 25, 2020

Sesquiterpene lactones, a vast range of terpenoids isolated from Asteraceae species, exhibit broad spectrum biological effects and several them are already commercially available, such as artemisinin. Here the most recent impactful results in vivo, preclinical clinical studies involving selection ten sesquiterpene lactones (alantolactone, arglabin, costunolide, cynaropicrin, helenalin, inuviscolide, lactucin, parthenolide, thapsigargin tomentosin) presented discussed, along with some their derivatives. In authors’ opinion, these compounds have been neglected compared to others, although they could be great use developing important new pharmaceutical products. The selected sesquiterpenes show promising anticancer anti-inflammatory effects, acting on various targets. Moreover, antifungal, anxiolytic, analgesic, antitrypanosomal activities. Several discussed here clearly potential that combination therapy, sensitizing agents facilitate enhance action drugs use. derivatives greater pharmacological value since better pharmacokinetics, stability, potency, and/or selectivity. All natural properties invite further research by scientific community.

Language: Английский

Citations

114
Reannotation of the cultivated strawberry genome and establishment of a strawberry genome database DOI
Tianjia Liu, Muzi Li, Zhongchi Liu

et al.

Horticulture Research, Journal Year: 2021, Volume and Issue: 8(1)

Published: March 1, 2021

Language: Английский

Citations

61
Melatonin alters the secondary metabolite profile of grape berry skin by promoting VvMYB14-mediated ethylene biosynthesis DOI
Wanyun Ma, Lili Xu, Shiwei Gao

et al.

Horticulture Research, Journal Year: 2021, Volume and Issue: 8(1)

Published: March 1, 2021

Language: Английский

Citations

60
Perspectives of CRISPR/Cas-mediated cis-engineering in horticulture: unlocking the neglected potential for crop improvement DOI Creative Commons
Qiang Li, Manoj Sapkota, Esther van der Knaap

et al.

Horticulture Research, Journal Year: 2020, Volume and Issue: 7(1)

Published: March 15, 2020

Directed breeding of horticultural crops is essential for increasing yield, nutritional content, and consumer-valued characteristics such as shape color the produce. However, limited genetic diversity restricts amount crop improvement that can be achieved through conventional approaches. Natural changes in

Language: Английский

Citations

65
Phytohormones: plant switchers in developmental and growth stages in potato DOI Creative Commons
Abbas Saidi,

Zahra Hajibarat

Journal of Genetic Engineering and Biotechnology, Journal Year: 2021, Volume and Issue: 19(1), P. 89 - 89

Published: June 17, 2021

Potato is one of the most important food crops worldwide, contributing key nutrients to human diet. Plant hormones act as vital switchers in regulation various aspects developmental and growth stages potato. Due broad impacts on many processes, their role potato has been investigated. This review presents a description hormonal basic pathways, interconnections between network reciprocal relationships, clarification molecular events underlying growth. In last decade, new findings have emerged regarding function during sprout development, vegetative growth, tuber initiation, maturation Hormones can control development potato, either individually or combination with other hormones. The characterization interplay cytokinins (CKs), abscisic acid (ABA), auxin and/or gibberellins (GAs) formation requires further undertaking. Recently, evidences relative functions an intricate several controlling are emerging. Although some widely covered, remarkable breaks our knowledge insights yet exist networks interactions different formation. present focuses roles view recognize mechanisms development. For better insight, relevant available interaction species also described. We predict that highlights conceptual developments associated downstream influencing

Language: Английский

Citations

53
The long noncoding RNA FRILAIR regulates strawberry fruit ripening by functioning as a noncanonical target mimic DOI Creative Commons
Yajun Tang, Zhipeng Qu, Jiajun Lei

et al.

PLoS Genetics, Journal Year: 2021, Volume and Issue: 17(3), P. e1009461 - e1009461

Published: March 19, 2021

Long noncoding RNAs (lncRNAs) are emerging as important regulators in plant development, but few of them have been functionally characterized fruit ripening. Here, we identified 25,613 lncRNAs from strawberry ripening fruits based on RNA-seq data poly(A)-depleted libraries and rRNA-depleted libraries, most which exhibited distinct temporal expression patterns. A novel lncRNA, FRILAIR harbours the miR397 binding site that is highly conserved diverse species. overexpression promoted maturation Falandi strawberry, was consistent with finding knocking down miR397, can guide mRNA cleavage both LAC11a (encoding a putative laccase-11-like protein). Moreover, levels were increased overexpressing knockdown fruits, accelerated also found fruits. Overall, our study demonstrates act noncanonical target mimic to modulate process.

Language: Английский

Citations

48
The AaCBF4-AaBAM3.1 module enhances freezing tolerance of kiwifruit (Actinidia arguta) DOI Creative Commons
Shihang Sun, Chun-Gen Hu,

Xiujuan Qi

et al.

Horticulture Research, Journal Year: 2021, Volume and Issue: 8(1)

Published: May 1, 2021

Abstract Beta-amylase (BAM) plays an important role in plant resistance to cold stress. However, the specific of BAM gene freezing tolerance is poorly understood. In this study, we demonstrated that a cold-responsive module was involved kiwifruit. module, expression AaBAM3.1 , which encodes functional protein, induced by -overexpressing kiwifruit lines showed increased tolerance, and heterologous overexpression Arabidopsis thaliana resulted similar phenotype. The results promoter GUS activity cis -element analyses predicted AaCBF4 be upstream transcription factor could regulate expression. Further investigation protein-DNA interactions using yeast one-hybrid, coexpression, dual luciferase reporter assays confirmed directly regulated addition, both responded positively Hence, conclude AaCBF-AaBAM positive regulation

Language: Английский

Citations

48
Molecular and genetic insights into secondary metabolic regulation underlying insect-pest resistance in legumes DOI
Muhammad Khuram Razzaq, Aiman Hina, Asim Abbasi

et al.

Functional & Integrative Genomics, Journal Year: 2023, Volume and Issue: 23(3)

Published: July 1, 2023

Language: Английский

Citations

15