A MYB activator, DcMYB11c, regulates carrot anthocyanins accumulation in petiole but not taproot DOI

Ao‐Qi Duan,

Yuan‐Jie Deng,

Shan‐Shan Tan

et al.

Plant Cell & Environment, Journal Year: 2023, Volume and Issue: 46(9), P. 2794 - 2809

Published: June 20, 2023

Abstract The first domesticated carrots were thought to be purple rich in anthocyanins. anthocyanins biosynthesis solid carrot taproot was regulated by DcMYB7 within P 3 region containing a gene cluster of six DcMYBs . Here, we described MYB the same region, DcMYB11c , which highly expressed pigmented petioles. Overexpression ‘Kurodagosun’ (KRD G orange with green petioles) and ‘Qitouhuang’ (QTH yellow resulted deep phenotype whole plants indicating accumulation. Knockout ‘Deep Purple’ (DPP through CRISPR/Cas9‐based genome editing pale due dramatic decrease content. could induce expression DcbHLH3 genes jointly promote biosynthesis. Yeast one‐hybrid assay (Y1H) dual‐luciferase reporter (LUC) revealed that bound promoters DcUCGXT1 DcSAT1 directly activated responsible for glycosylation acylation, respectively. Three transposons present cultivars petioles but not We core factor, DcMYB11c, involved pigmentation This study provides new insights into precise regulation mechanism underlying carrot. orchestrated might conserved across plant kingdom useful other researchers working on accumulation different tissues.

Language: Английский

Postharvest environmentally and human-friendly pre-treatments to minimize carrot waste in the supply chain caused by physiological disorders and fungi DOI Creative Commons
Konstantinos Papoutsis, Merete Edelenbos

Trends in Food Science & Technology, Journal Year: 2021, Volume and Issue: 112, P. 88 - 98

Published: March 28, 2021

Carrot is one of the most important horticultural crops, with an annual worldwide production exceeding 40 million tonnes. Carrots are sold either fresh intact or fresh-cut as minimally processed vegetables (MPV). In postharvest supply chain, physiological disorders, fungal decay, and their combinations reduce quality MPV carrots. carrots more susceptible to changes than due a higher loss protective epidermis, greater number wounded cells, increased respiration rates. The current review summarizes different environmentally human-friendly treatments applied in chain minimize adverse effects handling storage on disorders decay. Bitterness, white blush, browning critical Bitterness can be prevented by storing well-ventilated rooms without ethylene-producing fruit vegetables, while blush controlled application heat treatment, ultraviolet (UV)-irradiation, hydrogen sulfide (H2S), edible films. Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria radicina, Berkeleyomyces spp. (formerly Thielaviopsis spp.) fungi causing carrot losses waste. Fungal decay selecting healthy applying natural compounds, ozone (O3), UV-irradiation, inorganic salt, and/or biocontrol agents, combinations. However, successful combination sustainable treatment methods requires compatibility, -omics techniques may reveal best methods.

Language: Английский

Citations

34
Comparative assessment of volatile and phenolic profiles of fresh black carrot (Daucus carota L.) and powders prepared by three drying methods DOI
Muharrem Keskin, Gamze Güçlü, Yunus Emre Şekerli

et al.

Scientia Horticulturae, Journal Year: 2021, Volume and Issue: 287, P. 110256 - 110256

Published: May 23, 2021

Language: Английский

Citations

34
Overexpression of a carrot BCH gene, DcBCH1, improves tolerance to drought in Arabidopsis thaliana DOI Creative Commons
Tong Li,

Jie-Xia Liu,

Yuan‐Jie Deng

et al.

BMC Plant Biology, Journal Year: 2021, Volume and Issue: 21(1)

Published: Oct. 18, 2021

Abstract Background Carrot ( Daucus carota L.), an important root vegetable, is very popular among consumers as its taproot rich in various nutrients. Abiotic stresses, such drought, salt, and low temperature, are the main factors that restrict growth development of carrots. Non-heme carotene hydroxylase (BCH) a key regulatory enzyme β-branch carotenoid biosynthesis pathway, upstream abscisic acid (ABA) synthesis pathway. Results In this study, we characterized carrot BCH encoding gene, DcBCH1 . The expression was induced by drought treatment. overexpression Arabidopsis thaliana resulted enhanced tolerance to demonstrated higher antioxidant capacity lower malondialdehyde content after Under stress, endogenous ABA level transgenic A. than wild-type (WT) plants. Additionally, contents lutein β-carotene were those WT, whereas levels most carotenogenic genes significantly increased Conclusions can increase promote plants regulating rate carotenoids, thereby resistance These results will help provide potential candidate for plant breeding.

Language: Английский

Citations

34
Interactive Effects of Soil and Foliar-Applied Nanobiochar on Growth, Metabolites, and Nutrient Composition in Daucus carota DOI

Habiba Khaliq,

Sumera Anwar, Fahad Shafiq

et al.

Journal of Plant Growth Regulation, Journal Year: 2022, Volume and Issue: 42(6), P. 3715 - 3729

Published: Oct. 9, 2022

Language: Английский

Citations

23
A MYB activator, DcMYB11c, regulates carrot anthocyanins accumulation in petiole but not taproot DOI

Ao‐Qi Duan,

Yuan‐Jie Deng,

Shan‐Shan Tan

et al.

Plant Cell & Environment, Journal Year: 2023, Volume and Issue: 46(9), P. 2794 - 2809

Published: June 20, 2023

Abstract The first domesticated carrots were thought to be purple rich in anthocyanins. anthocyanins biosynthesis solid carrot taproot was regulated by DcMYB7 within P 3 region containing a gene cluster of six DcMYBs . Here, we described MYB the same region, DcMYB11c , which highly expressed pigmented petioles. Overexpression ‘Kurodagosun’ (KRD G orange with green petioles) and ‘Qitouhuang’ (QTH yellow resulted deep phenotype whole plants indicating accumulation. Knockout ‘Deep Purple’ (DPP through CRISPR/Cas9‐based genome editing pale due dramatic decrease content. could induce expression DcbHLH3 genes jointly promote biosynthesis. Yeast one‐hybrid assay (Y1H) dual‐luciferase reporter (LUC) revealed that bound promoters DcUCGXT1 DcSAT1 directly activated responsible for glycosylation acylation, respectively. Three transposons present cultivars petioles but not We core factor, DcMYB11c, involved pigmentation This study provides new insights into precise regulation mechanism underlying carrot. orchestrated might conserved across plant kingdom useful other researchers working on accumulation different tissues.

Language: Английский

Citations

16