A monomeric envelope glycoprotein cytoplasmic tail is sufficient for HIV-1 Gag lattice trapping and incorporation DOI Creative Commons
Nicholas S. Groves, Austin R. Clark,

Rebekah S. Aguilar

et al.

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

ABSTRACT To become infectious, assembling enveloped viruses must acquire viral glycoproteins to mediate downstream infection events. Human immunodeficiency virus-1 (HIV-1) envelope (Env) are well characterized function as trimers for membrane fusion and entry; however, we sought understand whether the trimeric structure of Env is required incorporation into virus particles. Using superresolution live-cell imaging biochemical assays, demonstrate that a monomeric receptor chimera containing cytoplasmic tail (Env-CT), known regulate incorporation, sufficient lattice trapping assembly sites. We also these Env-CT monomers can restrict native trimers, competing an apparently limited number interaction sites in each particle. Furthermore, this construct from evolutionarily distant HIV-1 primary isolate. Our findings support model where mediates with mechanism being conserved between clades HIV-1. IMPORTANCE combat prevalence antiviral resistance, new classes antivirals needed. An attractive target includes because released particles unable obtain non-infectious propagate infection. One requisite development targeting Gag-Env coalescence need define functional units constituting molecular interface. Although functions obligatory trimer entry, Monomeric displayed saturability lattices ability compete particle incorporation. These results suggest less complex mimetics could yield broad competitive activity against

Language: Английский

A Comprehensive Review of Fluorescence Correlation Spectroscopy DOI Creative Commons
Lan Yu, Yunze Lei, Ying Ma

et al.

Frontiers in Physics, Journal Year: 2021, Volume and Issue: 9

Published: April 12, 2021

Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of molecular dynamics, and it has been widely applied in diverse fields, e.g. , biomedicine, biophysics, chemistry. By time-correlation the fluorescence fluctuations induced by molecules diffusing through focused light, FCS can quantitatively evaluate concentration, diffusion coefficient, interaction vitro or vivo . In this review, basic principle implementation are introduced. Then, advances variants reviewed, covering dual-color FCCS, multi-focus FCS, pair function (pCF), scanning focus-reduced SPIM-FCS, inverse-FCS. Besides, applications demonstrated with measurement local hydrodynamic radius, different molecules. Lastly, discussion given summarizing pros cons techniques, as well outlooks perspectives FCS.

Language: Английский

Citations

161

Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans DOI

Melanie Koehler,

Martin Delguste, Christian Sieben

et al.

Annual Review of Virology, Journal Year: 2020, Volume and Issue: 7(1), P. 143 - 165

Published: May 12, 2020

Virus infection is an intricate process that requires the concerted action of both viral and host cell components. Entry viruses into cells initiated by interactions between proteins cell-surface receptors. Various glycans function as initial, usually low-affinity attachment factors, providing a first anchor virus to surface, further facilitate high-affinity binding virus-specific receptors, while other specific entry receptors themselves. It now possible rapidly identify glycan using different techniques, define atomic-level structures virus-glycan complexes, study these at single-virion level. This review provides detailed overview role in highlights experimental approaches along with examples. In particular, we highlight development atomic force microscope investigate level directly on living mammalian cells, which offers new perspectives better understand physiologically relevant conditions.

Language: Английский

Citations

124

Optical technologies for the detection of viruses like COVID-19: Progress and prospects DOI Creative Commons
Jijo Lukose, Santhosh Chidangil, Sajan D. George

et al.

Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 178, P. 113004 - 113004

Published: Jan. 18, 2021

Language: Английский

Citations

99

Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED–FCS DOI
Erdinç Sezgin, Falk Schneider, Silvia Galiani

et al.

Nature Protocols, Journal Year: 2019, Volume and Issue: unknown

Published: March 6, 2019

Language: Английский

Citations

98

Virus Impact on Lipids and Membranes DOI
Ellen Ketter, Glenn Randall

Annual Review of Virology, Journal Year: 2019, Volume and Issue: 6(1), P. 319 - 340

Published: Sept. 29, 2019

Viruses manipulate cellular lipids and membranes at each stage of their life cycle. This includes lipid-receptor interactions, the fusion viral envelopes with during endocytosis, reorganization to form replication compartments, envelopment egress virions. In addition physical interactions membranes, viruses have evolved lipid signaling metabolism benefit replication. review summarizes strategies that use in

Language: Английский

Citations

97

HIV-1 Gag specifically restricts PI(4,5)P2 and cholesterol mobility in living cells creating a nanodomain platform for virus assembly DOI Creative Commons
Cyril Favard, Jakub Chojnacki,

Peggy Mérida

et al.

Science Advances, Journal Year: 2019, Volume and Issue: 5(10)

Published: Oct. 2, 2019

HIV-1 Gag protein assembles at the plasma membrane of infected cells for viral particle formation. targets lipids, mainly PI(4,5)P2, inner leaflet this membrane. Here, we address question whether is able to trap specifically PI(4,5)P2 or other lipids during assembly in host CD4

Language: Английский

Citations

79

Measuring plasma membrane fluidity using confocal microscopy DOI
Pablo Carravilla, Luca Andronico, Jan Schlegel

et al.

Nature Protocols, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

Citations

1

Between life and death: strategies to reduce phototoxicity in super-resolution microscopy DOI Creative Commons
Kalina L. Tosheva, Yue Yuan, Pedro M. Pereira

et al.

Journal of Physics D Applied Physics, Journal Year: 2020, Volume and Issue: 53(16), P. 163001 - 163001

Published: Jan. 15, 2020

Abstract Super-resolution microscopy (SRM) enables non-invasive, molecule-specific imaging of the internal structure and dynamics cells with sub-diffraction limit spatial resolution. One its major limitations is requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits capacity live-cell observations, particularly extended periods time. Here, we give an overview new developments in hardware, software probe chemistry aiming to reduce Additionally, discuss how choice biological model sample environment impacts observations.

Language: Английский

Citations

67

Electron tomography visualization of HIV-1 fusion with target cells using fusion inhibitors to trap the pre-hairpin intermediate DOI Creative Commons
Mark S. Ladinsky,

Priyanthi N.P. Gnanapragasam,

Zhi Yang

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: July 22, 2020

Fusion of HIV-1 with the membrane its target cell, an obligate first step in virus infectivity, is mediated by binding viral envelope (Env) spike protein to receptors, CD4 and CCR5/CXCR4, on cell surface. The process fusion appears be fast compared egress has not been visualized EM. To capture events, must curtailed trapping Env-receptor at intermediate stage. We have used inhibitors trap virions attached cells Envs extended pre-hairpin state. Electron tomography revealed bound TZM-bl 2-4 narrow spokes, slightly more spokes present when evaluated mutant that lacked Env cytoplasmic tail. These results represent direct visualization hypothesized improve our understanding Env-mediated infection host cells.

Language: Английский

Citations

58

Influence of membrane on the antigen presentation of the HIV-1 envelope membrane proximal external region (MPER) DOI
César A. López,

S. Munir Alam,

Cynthia A. Derdeyn

et al.

Current Opinion in Structural Biology, Journal Year: 2024, Volume and Issue: 88, P. 102897 - 102897

Published: Aug. 22, 2024

Language: Английский

Citations

7