Frontiers in Microbiology,
Journal Year:
2020,
Volume and Issue:
11
Published: Feb. 12, 2020
Biodegradation
of
synthetic
polymers,
in
particular
polyethylene
terephthalate
(PET),
is
great
importance,
since
environmental
pollution
with
PET
and
other
plastics
has
become
a
severe
global
problem.
Here,
we
report
on
the
polyester
degrading
ability
novel
carboxylic
ester
hydrolase
identified
genome
marine
hydrocarbonoclastic
bacterium
Pseudomonas
aestusnigri
VGXO14
T
.
The
enzyme,
designated
PE-H,
belongs
to
type
IIa
family
hydrolytic
enzymes
as
indicated
by
amino
acid
sequence
homology.
It
was
produced
Escherichia
coli,
purified
its
crystal
structure
solved
at
1.09
Å
resolution
representing
first
enzyme.
shows
typical
α/β-hydrolase
fold
high
structural
homology
known
hydrolases.
hydrolysis
detected
30°C
amorphous
film
(PETa),
but
not
from
commercial
bottle
(PETb).
A
rational
mutagenesis
study
improve
potential
PE-H
yielded
variant
(Y250S)
which
showed
improved
activity,
ultimately
also
allowing
PETb.
this
1.35
allowed
rationalize
improvement
enzymatic
activity.
oligomer
binding
model
proposed
molecular
docking
computations.
Our
results
indicate
significant
P.
for
degradation.
Microbial Cell Factories,
Journal Year:
2019,
Volume and Issue:
18(1)
Published: Oct. 10, 2019
Abstract
Background
The
biological
degradation
of
plastics
is
a
promising
method
to
counter
the
increasing
pollution
our
planet
with
artificial
polymers
and
develop
eco-friendly
recycling
strategies.
Polyethylene
terephthalate
(PET)
thermoplast
industrially
produced
from
fossil
feedstocks
since
1940s,
nowadays
prevalently
used
in
bottle
packaging
textiles.
Although
established
industrial
processes
for
PET
exist,
large
amounts
still
end
up
environment—a
significant
portion
thereof
world’s
oceans.
In
2016,
Ideonella
sakaiensis
,
bacterium
possessing
ability
degrade
use
products
as
sole
carbon
source
growth,
was
isolated.
I.
expresses
key
enzyme
responsible
breakdown
into
monomers:
PETase.
This
hydrolase
might
possess
huge
potential
development
well
bioremediation
approaches
environmental
plastic
waste.
Results
Using
photosynthetic
microalga
Phaeodactylum
tricornutum
chassis
we
generated
microbial
cell
factory
capable
producing
secreting
an
engineered
version
PETase
surrounding
culture
medium.
Initial
experiments
using
supernatant
at
30
°C
showed
that
possessed
activity
against
copolymer
polyethylene
glycol
(PETG)
approximately
80-fold
higher
turnover
low
crystallinity
PETG
compared
PET.
Moreover,
show
diatom
active
shredded
saltwater-based
environment
even
mesophilic
temperatures
(21
°C).
resulting
substrate
were
mainly
terephthalic
acid
(TPA)
mono(2-hydroxyethyl)
(MHET)
estimated
be
formed
micromolar
range
under
selected
reaction
conditions.
Conclusion
We
provide
solution
decomposition
waste
by
eukaryotic
instead
model
system.
Our
results
via
synthetic
biology
P.
indeed
could
converted
valuable
degradation.
Overall,
this
proof
principle
study
demonstrates
system
future
biotechnological
applications
especially
polluted
seawater.
Environmental Science & Technology,
Journal Year:
2019,
Volume and Issue:
53(5), P. 2304 - 2315
Published: Jan. 30, 2019
The
agricultural
use
of
conventional,
polyethylene-based
mulch
films
leads
to
the
accumulation
remnant
film
pieces
in
soils
with
negative
impacts
for
soil
productivity
and
ecology.
A
viable
strategy
overcome
this
is
replace
conventional
biodegradable
composed
polymers
designed
be
degraded
by
microorganisms.
However,
understanding
polymer
biodegradation
remains
a
significant
challenge
due
its
dependence
on
properties,
characteristics,
prevailing
environmental
conditions.
This
perspective
aims
advance
our
three
fundamental
steps
underlying
soils:
colonization
surfaces
microorganisms,
depolymerization
extracellular
microbial
hydrolases,
subsequent
assimilation
utilization
hydrolysis
products
energy
production
biomass
formation.
synthesizes
current
conceptual
these
highlights
existing
knowledge
gaps.
discussion
addresses
future
research
analytical
advancements
required
gaps
identify
key
properties
characteristics
governing
soils.
Limnology and Oceanography Letters,
Journal Year:
2020,
Volume and Issue:
5(1), P. 18 - 36
Published: Jan. 22, 2020
Abstract
Microorganisms
drive
the
biogeochemical
cycles
that
link
abiotic
and
biotic
processes
in
aqueous
environment
are
intricately
associated
with
plastic
debris.
The
presence
of
microplastics
water
sediment
introduces
new
concerns
as
small
particle
size
allows
for
increased
pathways
food
web
element
cycles.
In
this
review,
we
present
current
state
knowledge
on
microbe‐plastic
interactions
summarize
potential
impact
distribution,
cycling,
transport,
sedimentation.
We
explore
how
influence
exposure
consumers
to
degradation
products.
Key
methods
used
elucidate
biofilm
development,
microbial
biodegradation,
microplastic
detection
discussed.
Finally,
comment
future
questions
research
directions
needed
further
define
role
microorganisms
environmental
fate
microplastics.
Frontiers in Microbiology,
Journal Year:
2020,
Volume and Issue:
11
Published: Feb. 12, 2020
Biodegradation
of
synthetic
polymers,
in
particular
polyethylene
terephthalate
(PET),
is
great
importance,
since
environmental
pollution
with
PET
and
other
plastics
has
become
a
severe
global
problem.
Here,
we
report
on
the
polyester
degrading
ability
novel
carboxylic
ester
hydrolase
identified
genome
marine
hydrocarbonoclastic
bacterium
Pseudomonas
aestusnigri
VGXO14
T
.
The
enzyme,
designated
PE-H,
belongs
to
type
IIa
family
hydrolytic
enzymes
as
indicated
by
amino
acid
sequence
homology.
It
was
produced
Escherichia
coli,
purified
its
crystal
structure
solved
at
1.09
Å
resolution
representing
first
enzyme.
shows
typical
α/β-hydrolase
fold
high
structural
homology
known
hydrolases.
hydrolysis
detected
30°C
amorphous
film
(PETa),
but
not
from
commercial
bottle
(PETb).
A
rational
mutagenesis
study
improve
potential
PE-H
yielded
variant
(Y250S)
which
showed
improved
activity,
ultimately
also
allowing
PETb.
this
1.35
allowed
rationalize
improvement
enzymatic
activity.
oligomer
binding
model
proposed
molecular
docking
computations.
Our
results
indicate
significant
P.
for
degradation.
