Organic Letters,
Journal Year:
2024,
Volume and Issue:
26(20), P. 4302 - 4307
Published: May 10, 2024
A
plant
used
in
an
Indonesian
traditional
herbal
medicine
as
a
diabetes
treatment
and
known
locally
"Jampu
Salo"
was
collected
on
Sulawesi
Island,
Indonesia.
It
identified
Syzygium
oblanceolatum
(C.
B.
Rob.)
Merr.
(Myrtaceae)
found
for
the
first
time
Sulawesi;
it
previously
reported
only
eastern
Philippines
Borneo.
phytochemical
study
of
S.
led
to
isolation
three
unprecedented
meroterpenoids,
syzygioblanes
A–C
(1–3,
respectively).
These
compounds
might
be
biosynthesized
through
[4+2]
cycloaddition
various
germacrane-based
cyclic
sesquiterpenoids
with
flavone
desmethoxymatteucinol
form
spiro
skeleton.
The
unique
complex
structures
were
elucidated
by
microcrystal
electron
diffraction
analysis
addition
general
analytical
techniques
such
high-resolution
mass
spectrometry,
nuclear
magnetic
resonance
methods,
infrared
spectroscopy.
Synchrotron
X-ray
calculations
electronic
circular
dichroism
spectra
helped
determine
absolute
configurations.
newly
isolated
exhibited
collateral
sensitivity
more
strongly
inhibit
growth
multidrug
resistant
tumor
cell
line
compared
chemosensitive
line.
IMA Fungus,
Journal Year:
2021,
Volume and Issue:
12(1)
Published: Aug. 9, 2021
ABSTRACT
Marine
fungi
remain
poorly
covered
in
global
genome
sequencing
campaigns;
the
1000
fungal
genomes
(1KFG)
project
attempts
to
shed
light
on
diversity,
ecology
and
potential
industrial
use
of
overlooked
resolved
taxa.
This
study
characterizes
three
marine
fungi:
Emericellopsis
sp.
TS7,
wood-associated
Amylocarpus
encephaloides
algae-associated
Calycina
marina.
These
species
were
sequenced
their
genomic
features,
biosynthetic
phylogenetic
placement
using
multilocus
data.
C.
marina
placed
Helotiaceae
Pezizellaceae
(Helotiales)
,
respectively,
based
a
15-gene
analysis.
two
had
fewer
gene
clusters
(BGCs)
carbohydrate
active
enzymes
(CAZymes)
than
TS7
isolate.
(
Hypocreales
Ascomycota
)
was
isolated
from
sponge
Stelletta
normani
.
A
six-gene
analysis
isolate
clade
morphological
examination
confirmed
that
represents
new
species,
which
is
described
here
as
E.
atlantica
Analysis
its
CAZyme
repertoire
culturing
experiment
one
terrestrial
substrates
indicated
psychrotrophic
generalist
fungus
able
degrade
several
types
biomass.
FungiSMASH
revealed
presence
35
BGCs
including,
eight
non-ribosomal
peptide
synthases
(NRPSs),
six
NRPS-like,
polyketide
synthases,
nine
terpenes
hybrid,
mixed
or
other
clusters.
Of
these
BGCs,
only
five
homologous
with
characterized
BGCs.
The
unknown
sets
large
set
stage
for
further
investigations
monotypic
genus
represent
first
published
filamentous
are
restricted
occurrence
habitat
form
thus
valuable
resource
community
can
be
used
studying
ecological
adaptions
comparative
genomics.
Natural Product Reports,
Journal Year:
2022,
Volume and Issue:
40(1), P. 28 - 45
Published: May 23, 2022
This
review
summarises
recent
research
on
metobolic
engineering
approaches
towards
terpenes,
including
work
to
investigate
unknown
pathways
and
improve
accessibility
of
known
compounds.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(52)
Published: Nov. 14, 2023
The
diterpene
synthase
AlTS
was
identified
from
Aspergillus
luchuensis.
catalyses
the
formation
of
hydrocarbon
spiroluchuene
A,
which
exhibits
a
novel
skeleton
characterised
by
spirocyclic
ring
system.
cyclisation
mechanism
towards
this
compound
elucidated
through
isotopic
labelling
experiments
in
conjunction
with
DFT
calculations
and
metadynamic
simulations.
biosynthetic
intermediate
luchudiene,
besides
derivative
B,
captured
an
enzyme
variant
obtained
site-directed
mutagenesis.
With
its
10-membered
luchudiene
is
structurally
related
to
germacrenes
can
undergo
Cope
rearrangement
luchuelemene.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(41)
Published: Aug. 30, 2023
Fungal
hybrid
terpenoid
saccharides
constitute
a
new
and
growing
family
of
natural
products
with
significant
biomedical
agricultural
activities.
One
representative
is
the
cosmosporasides,
which
feature
oxidized
units
saccharide
moieties;
however,
assembly
line
these
building
blocks
has
been
elusive.
Herein,
cos
cluster
from
Fusarium
orthoceras
was
discovered
for
synthesis
cosmosporaside
C
(1)
by
genome
mining.
A
UbiA
intramembrane
prenyltransferase
(UbiA-type
PT),
multifunctional
cytochrome
P450,
an
α,β-hydrolase,
acetyltransferase,
dimethylallyl
transferase
(DMAT-type
PT)
glycosyltransferase
function
cooperatively
in
scaffold
1
using
primary
central
metabolites.
The
absolute
configuration
at
C4,
C6
C7
also
established.
Our
work
clarifies
unexpected
functions
UbiA-type
DMAT-type
PTs
provides
example
understanding
synthetic
logic
fungi.
Organic Letters,
Journal Year:
2024,
Volume and Issue:
26(20), P. 4302 - 4307
Published: May 10, 2024
A
plant
used
in
an
Indonesian
traditional
herbal
medicine
as
a
diabetes
treatment
and
known
locally
"Jampu
Salo"
was
collected
on
Sulawesi
Island,
Indonesia.
It
identified
Syzygium
oblanceolatum
(C.
B.
Rob.)
Merr.
(Myrtaceae)
found
for
the
first
time
Sulawesi;
it
previously
reported
only
eastern
Philippines
Borneo.
phytochemical
study
of
S.
led
to
isolation
three
unprecedented
meroterpenoids,
syzygioblanes
A–C
(1–3,
respectively).
These
compounds
might
be
biosynthesized
through
[4+2]
cycloaddition
various
germacrane-based
cyclic
sesquiterpenoids
with
flavone
desmethoxymatteucinol
form
spiro
skeleton.
The
unique
complex
structures
were
elucidated
by
microcrystal
electron
diffraction
analysis
addition
general
analytical
techniques
such
high-resolution
mass
spectrometry,
nuclear
magnetic
resonance
methods,
infrared
spectroscopy.
Synchrotron
X-ray
calculations
electronic
circular
dichroism
spectra
helped
determine
absolute
configurations.
newly
isolated
exhibited
collateral
sensitivity
more
strongly
inhibit
growth
multidrug
resistant
tumor
cell
line
compared
chemosensitive
line.
