Advanced Science,
Journal Year:
2023,
Volume and Issue:
10(11)
Published: Jan. 20, 2023
As
a
key
step
of
tumor
lymphatic
metastasis,
lymphangiogenesis
is
regulated
by
VEGFC-VEGFR3
signaling
pathway
mediated
immune
cells,
mainly
macrophages,
in
the
microenvironment.
However,
little
known
whether
associated
neutrophils
are
involved
lymphangiogenesis.
Here,
it
found
that
TANs
infiltration
increased
LN-metastatic
BCa
and
with
poor
prognosis.
Neutrophil
depletion
results
significant
reduction
popliteal
LN
metastasis
Mechanistically,
transcription
factor
ETV4
enhances
cells-derived
CXCL1/8
to
recruit
TANs,
leading
increase
VEGFA
MMP9
from
then
facilitating
BCa.
Moreover,
phosphorylation
at
tyrosine
392
kinase
PTK6
increases
nuclear
translocation
essential
for
its
function
Overall,
findings
reveal
novel
mechanism
how
cells
regulate
TANs-induced
identify
as
therapeutic
target
Molecular Cancer,
Journal Year:
2021,
Volume and Issue:
20(1)
Published: Oct. 11, 2021
Abstract
Cancer-associated
fibroblasts
(CAFs),
a
stromal
cell
population
with
cell-of-origin,
phenotypic
and
functional
heterogeneity,
are
the
most
essential
components
of
tumor
microenvironment
(TME).
Through
multiple
pathways,
activated
CAFs
can
promote
growth,
angiogenesis,
invasion
metastasis,
along
extracellular
matrix
(ECM)
remodeling
even
chemoresistance.
Numerous
previous
studies
have
confirmed
critical
role
interaction
between
cells
in
tumorigenesis
development.
However,
recently,
mutual
effects
immune
(TIME)
been
identified
as
another
key
factor
promoting
progression.
The
TIME
mainly
consists
distinct
populations
islets
is
highly
associated
antitumor
immunological
state
TME.
interact
tumor-infiltrating
well
other
within
via
secretion
various
cytokines,
growth
factors,
chemokines,
exosomes
effector
molecules,
consequently
shaping
an
immunosuppressive
TME
that
enables
cancer
to
evade
surveillance
system.
In-depth
interactions,
particularly
complicated
mechanisms
connecting
cells,
might
provide
novel
strategies
for
subsequent
targeted
immunotherapies.
Herein,
we
shed
light
on
recent
advances
regarding
direct
indirect
crosstalk
infiltrating
further
summarize
possible
immunoinhibitory
induced
by
In
addition,
present
current
related
CAF-targeting
immunotherapies
briefly
describe
some
future
perspectives
CAF
research
end.
Journal of Hematology & Oncology,
Journal Year:
2021,
Volume and Issue:
14(1)
Published: June 9, 2021
Single-cell
sequencing,
including
genomics,
transcriptomics,
epigenomics,
proteomics
and
metabolomics
is
a
powerful
tool
to
decipher
the
cellular
molecular
landscape
at
single-cell
resolution,
unlike
bulk
which
provides
averaged
data.
The
use
of
sequencing
in
cancer
research
has
revolutionized
our
understanding
biological
characteristics
dynamics
within
lesions.
In
this
review,
we
summarize
emerging
technologies
recent
progress
obtained
by
information
related
landscapes
malignant
cells
immune
cells,
tumor
heterogeneity,
circulating
underlying
mechanisms
behaviors.
Overall,
prospects
facilitating
diagnosis,
targeted
therapy
prognostic
prediction
among
spectrum
tumors
are
bright.
near
future,
advances
will
undoubtedly
improve
highlight
potential
precise
therapeutic
targets
for
patients.
Nature Communications,
Journal Year:
2022,
Volume and Issue:
13(1)
Published: Nov. 4, 2022
Abstract
Cancer-associated
fibroblasts
(CAFs)
are
the
predominant
components
of
tumor
microenvironment
(TME)
and
influence
cancer
hallmarks,
but
without
systematic
investigation
on
their
ubiquitous
characteristics
across
different
types.
Here,
we
perform
pan-cancer
analysis
226
samples
10
solid
types
to
profile
TME
at
single-cell
resolution,
illustrating
commonalities/plasticity
heterogenous
CAFs.
Activation
trajectory
major
CAF
is
divided
into
three
states,
exhibiting
distinct
interactions
with
other
cell
components,
relating
prognosis
immunotherapy.
Moreover,
minor
represent
alternative
origin
from
(e.g.,
endothelia
macrophages).
Particularly,
presentation
endothelial-to-mesenchymal
transition
CAF,
which
may
interact
proximal
SPP
1
+
tumor-associated
macrophages,
implicated
in
survival
stratifications.
Our
study
comprehensively
profiles
shared
dynamics
CAFs,
highlight
heterogeneity
plasticity
Browser
integrated
information
available
https://gist-fgl.github.io/sc-caf-atlas/
.
Biomarker Research,
Journal Year:
2020,
Volume and Issue:
8(1)
Published: Nov. 11, 2020
Cancer-associated
fibroblasts
(CAFs)
are
the
key
component
of
tumor
stromal.
High
heterogeneity
CAFs
reflects
in
their
origin,
phenotype
and
function.
Biological
function
which
can
be
suggested
by
biomarkers
distinct
CAF
subgroups
may
different,
even
opposite,
just
like
water
fire.
Identifying
subpopulations
expressing
different
reconciling
relationship
"water
fire"
among
subsets
a
breakthrough
therapy.
Herein,
we
briefly
summarize
commonly
used
or
newly
identified
for
terms
features
potential
clinical
benefits.
International Journal of Molecular Sciences,
Journal Year:
2021,
Volume and Issue:
22(11), P. 5421 - 5421
Published: May 21, 2021
Inflammation,
especially
chronic
inflammation,
plays
a
pivotal
role
in
tumorigenesis
and
metastasis
through
various
mechanisms
is
now
recognized
as
hallmark
of
cancer
an
attractive
therapeutic
target
cancer.
In
this
review,
we
discuss
recent
advances
molecular
how
inflammation
promotes
suppresses
anti-tumor
immunity
types
solid
tumors,
including
esophageal,
gastric,
colorectal,
liver,
pancreatic
well
hematopoietic
malignancies.
Cancer Discovery,
Journal Year:
2022,
Volume and Issue:
12(11), P. 2606 - 2625
Published: Aug. 26, 2022
Abstract
It
is
currently
accepted
that
cancer-associated
fibroblasts
(CAF)
participate
in
T-cell
exclusion
from
tumor
nests.
To
unbiasedly
test
this,
we
used
single-cell
RNA
sequencing
coupled
with
multiplex
imaging
on
a
large
cohort
of
lung
tumors.
We
identified
four
main
CAF
populations,
two
which
are
associated
exclusion:
(i)
MYH11+αSMA+
CAF,
present
early-stage
tumors
and
form
single
cell
layer
lining
cancer
aggregates,
(ii)
FAP+αSMA+
appear
more
advanced
organize
patches
within
the
stroma
or
multiple
layers
around
Both
populations
orchestrate
particular
structural
tissue
organization
through
dense
aligned
fiber
deposition
compared
T
cell–permissive
CAF.
Yet
they
produce
distinct
matrix
molecules,
including
collagen
IV
(MYH11+αSMA+
CAF)
XI/XII
(FAP+αSMA+
CAF).
Hereby,
uncovered
unique
molecular
programs
driving
marginalization,
whose
targeting
should
increase
immunotherapy
efficacy
patients
bearing
cell–excluded
Significance:
The
cellular
marginalization
solid
remain
unclear.
Here,
describe
human
demonstrate
importance
pairing
spatial
analysis
microenvironment,
prerequisite
to
developing
new
strategies
cell–excluding
See
related
commentary
by
Sherman,
p.
2501.
This
article
highlighted
In
Issue
feature,
2483
International Journal of Biological Sciences,
Journal Year:
2022,
Volume and Issue:
18(7), P. 3019 - 3033
Published: Jan. 1, 2022
Tumor
heterogeneity
is
one
of
the
hallmarks
cancer
and
a
challenge
in
field
oncology.Tumor
main
cause
drug
resistance,
leading
to
therapeutic
failure.Mechanically,
tumor
either
directly
affects
targets
or
shapes
microenvironment
(TME)
by
defining
transcriptomic
phenotypic
profiles
influence
resistance.Tumor
evolves
spatially
temporally
during
development,
constant
reprogramming
TME.Advances
molecular
profiling
technologies
precision
oncology
platforms
have
allowed
us
uncover
impact
on
resistance
context
TME.In
this
review,
we
focus
processes
which
genomic
mutations
drive
mechanisms
through
reprograms
TME
affect
patient
prognosis.
Clinical and Translational Medicine,
Journal Year:
2022,
Volume and Issue:
12(2)
Published: Feb. 1, 2022
Abstract
Background
Deciphering
intra‐
and
inter‐tumoural
heterogeneity
is
essential
for
understanding
the
biology
of
gastric
cancer
(GC)
its
metastasis
identifying
effective
therapeutic
targets.
However,
characteristics
different
organ‐tropism
metastases
GC
are
largely
unknown.
Methods
Ten
fresh
human
tissue
samples
from
six
patients,
including
primary
tumour
adjacent
non‐tumoural
organs
or
tissues
(liver,
peritoneum,
ovary,
lymph
node)
were
evaluated
using
single‐cell
RNA
sequencing.
Validation
experiments
performed
histological
assays
bulk
transcriptomic
datasets.
Results
Malignant
epithelial
subclusters
associated
with
invasion
features,
intraperitoneal
propensity,
epithelial–mesenchymal
transition‐induced
stem
cell
phenotypes,
dormancy‐like
discovered.
High
expression
first
three
subcluster‐associated
genes
displayed
worse
overall
survival
than
those
low
in
a
cohort
containing
407
samples.
Immune
stromal
cells
exhibited
cellular
created
pro‐tumoural
immunosuppressive
microenvironment.
Furthermore,
20‐gene
signature
node‐derived
exhausted
CD8
+
T
was
acquired
to
forecast
node
validated
cohorts.
Additionally,
although
anti‐NKG2A
(KLRC1)
antibody
have
not
been
used
treat
patients
even
clinical
trials,
we
uncovered
only
malignant
but
one
endothelial
subcluster,
mucosal‐associated
invariant
cells,
cell‐like
B
plasmacytoid
dendritic
macrophages,
monocytes,
neutrophils
may
contribute
HLA‐E‐KLRC1/KLRC2
interaction
cytotoxic/exhausted
and/or
natural
killer
(NK)
suggesting
novel
opportunities
GC.
our
findings
suggested
that
PD‐1
might
predict
responses
blockade
therapy
Conclusions
This
study
provided
insights
into
heterogeneous
microenvironment
tumours
organ‐specific
provide
support
precise
diagnosis
treatment.