Reproduction Fertility and Development,
Journal Year:
2022,
Volume and Issue:
35(2), P. 118 - 124
Published: Oct. 19, 2022
Emerging
evidence
now
shows
that
in
addition
to
delivering
a
haploid
DNA,
the
mammalian
sperm
also
carry
various
types
of
RNAs
respond
paternal
environment,
which
can
mediate
intergenerational
transmission
certain
phenotypes
offspring
relating
environmental
exposures
(e.g.
diet,
mental
stress).
Improved
analytical
tools
are
beginning
decipher
complexity
RNAs,
RNA
modifications
and
their
spatial
compartmentalisation,
support
concept
'sperm
code'
programming
specific
during
embryonic
development.
In
this
commentary
article,
I
discuss
challenges
opportunities
solidifying
field
RNA-mediated
epigenetic
inheritance,
including
identification
key
responsible
for
phenotype
transmission,
cellular
molecular
events
triggered
by
embryo
translational
application
potential
harnessing
knowledge
code
improve
farm
animal
production
human
health.
Nature Communications,
Journal Year:
2021,
Volume and Issue:
12(1)
Published: Oct. 13, 2021
PIWI-interacting
small
RNAs
(piRNAs)
protect
the
germline
genome
and
are
essential
for
fertility.
piRNAs
originate
from
transposable
element
(TE)
RNAs,
long
non-coding
or
3´
untranslated
regions
(3´UTRs)
of
protein-coding
messenger
genes,
with
last
being
least
characterized
three
piRNA
classes.
Here,
we
demonstrate
that
precursors
3´UTR
full-length
mRNAs
post-termination
80S
ribosomes
guide
production
on
3´UTRs
in
mice
chickens.
At
pachytene
stage,
when
other
co-translational
RNA
surveillance
pathways
sequestered,
biogenesis
degrades
right
after
pioneer
rounds
translation
fine-tunes
protein
mRNAs.
Although
precursor
code
distinct
proteins
chickens,
they
all
harbor
embedded
TEs
produce
cleave
TEs.
Altogether,
discover
a
function
pathway
fine-tuning
reveal
conserved
mechanism
recognizes
translating
amniotes.
Scientific Reports,
Journal Year:
2022,
Volume and Issue:
12(1)
Published: Aug. 19, 2022
In
non-mammalian
vertebrates,
the
molecular
mechanisms
involved
in
transformation
of
haploid
germ
cells
(HGCs)
into
spermatozoa
(spermiogenesis)
are
largely
unknown.
Here,
we
investigated
this
process
marine
teleost
gilthead
seabream
(Sparus
aurata)
through
examination
changes
transcriptome
between
cell-sorted
HGCs
and
ejaculated
sperm
(SPZEJ).
Samples
were
collected
under
strict
quality
controls
employing
immunofluorescence
microscopy
as
well
by
determining
motion
kinematic
parameters
computer-assisted
analysis.
Deep
sequencing
RNA-seq
identified
a
total
7286
differentially
expressed
genes
(DEGs)
(p-value
<
0.01)
both
cell
types,
which
nearly
half
upregulated
SPZEJ
compared
to
HCGs.
addition,
approximately
9000
long
non-coding
RNAs
(lncRNAs)
found,
56%
accumulated
or
emerged
de
novo
SPZEJ.
The
transcripts
transcriptional
translational
regulation,
chromatin
cytoskeleton
organization,
metabolic
processes
such
glycolysis
oxidative
phosphorylation,
also
include
number
ion
water
channels,
exchangers,
transporters
receptors.
Pathway
analysis
conducted
on
DEGs
37
different
signaling
pathways
enriched
SPZEJ,
including
13
receptor
pathways,
from
most
predominant
correspond
chemokine
cytokine,
gonadotropin-releasing
hormone
platelet
derived
growth
factor
pathways.
Our
data
provide
new
insight
mRNA
lncRNA
cargos
uncover
possible
involvement
novel
endocrine
during
differentiation
maturation
spermatozoa.
Current Zoology,
Journal Year:
2022,
Volume and Issue:
8(1)
Published: Jan. 1, 2022
The
inheritance
of
neurophysiologic
and
neuropsychologic
complex
diseases
can
only
partly
be
explained
by
the
Mendelian
concept
genetic
inheritance.
Previous
research
showed
that
both
psychological
disorders
like
post-traumatic
stress
disorder
metabolic
are
more
prevalent
in
progeny
affected
parents.
This
could
suggest
an
epigenetic
mode
transmission.
Human
studies
give
first
insight
into
scope
intergenerational
influence
stressors
but
limited
exploring
underlying
mechanisms.
Animal
models
have
elucidated
mechanistic
underpinnings
In
this
review,
we
summarize
progress
on
mechanisms
paternal
transmission
means
sperm
RNA
mouse
models.
We
discuss
relevant
details
for
modelling
RNA-mediated
transmission,
point
towards
currently
unanswered
questions
propose
experimental
considerations
tackling
these
questions.
Reproduction Fertility and Development,
Journal Year:
2022,
Volume and Issue:
35(2), P. 118 - 124
Published: Oct. 19, 2022
Emerging
evidence
now
shows
that
in
addition
to
delivering
a
haploid
DNA,
the
mammalian
sperm
also
carry
various
types
of
RNAs
respond
paternal
environment,
which
can
mediate
intergenerational
transmission
certain
phenotypes
offspring
relating
environmental
exposures
(e.g.
diet,
mental
stress).
Improved
analytical
tools
are
beginning
decipher
complexity
RNAs,
RNA
modifications
and
their
spatial
compartmentalisation,
support
concept
'sperm
code'
programming
specific
during
embryonic
development.
In
this
commentary
article,
I
discuss
challenges
opportunities
solidifying
field
RNA-mediated
epigenetic
inheritance,
including
identification
key
responsible
for
phenotype
transmission,
cellular
molecular
events
triggered
by
embryo
translational
application
potential
harnessing
knowledge
code
improve
farm
animal
production
human
health.
