The central nucleus of the amygdala and the construction of defensive modes across the threat-imminence continuum

Nature Neuroscience, Journal Year: 2022, Volume and Issue: 25(8), P. 999 - 1008

Published: Aug. 1, 2022

Language: Английский

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Reward and aversion processing by input-defined parallel nucleus accumbens circuits in mice

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Oct. 21, 2022

Abstract The nucleus accumbens (NAc) is critical in mediating reward seeking and also involved negative emotion processing, but the cellular circuitry mechanisms underlying such opposing behaviors remain elusive. Here, using recently developed AAV1-mediated anterograde transsynaptic tagging technique mice, we show that NAc neurons receiving basolateral amygdala inputs (NAc BLA ) promote positive reinforcement via disinhibiting dopamine ventral tegmental area (VTA). In contrast, paraventricular thalamic PVT innervate GABAergic lateral hypothalamus (LH) mediate aversion. Silencing synaptic output of impairs behavior, while silencing or →LH pathway abolishes aversive symptoms opiate withdrawal. Our results elucidate afferent-specific circuit architecture controlling

Language: Английский

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Two opposing hippocampus to prefrontal cortex pathways for the control of approach and avoidance behaviour

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Jan. 17, 2022

Abstract The decision to either approach or avoid a potentially threatening environment is thought rely upon the coordinated activity of heterogeneous neural populations in hippocampus and prefrontal cortex (PFC). However, how this circuitry organized flexibly promote both avoidance at different times has remained elusive. Here, we show that hippocampal projection PFC composed two parallel circuits located superficial deep pyramidal layers CA1/subiculum border. These have unique upstream downstream connectivity, are differentially active during behaviour. population preferentially connected widespread inhibitory interneurons, its activation promotes exploration; while circuit neurons fast spiking avoidance. Together provides mechanism for regulation behaviour conflict: through specialized, allow bidirectional control PFC.

Language: Английский

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PBN-PVT projections modulate negative affective states in mice

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Feb. 15, 2022

Long-lasting negative affections dampen enthusiasm for life, and dealing with affective states is essential individual survival. The parabrachial nucleus (PBN) thalamic paraventricular (PVT) are critical modulating in mice. However, the functional roles of PBN-PVT projections remain elusive. Here, we show that PBN neurons send dense projection fibers to PVT form direct excitatory synapses neurons. Activation pathway induces robust behaviors associated without affecting nociceptive behaviors. Inhibition reduces aversion-like fear-like Furthermore, innervated by activated aversive stimulation, activation enhances neuronal activity response stimulus. Consistently, received anxiety-like Thus, our study indicates modulate

Language: Английский

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Molecular and spatial profiling of the paraventricular nucleus of the thalamus

eLife, Journal Year: 2023, Volume and Issue: 12

Published: March 3, 2023

The paraventricular nucleus of the thalamus (PVT) is known to regulate various cognitive and behavioral processes. However, while functional diversity among PVT circuits has often been linked cellular differences, molecular identity spatial distribution cell types remain unclear. To address this gap, here we used single RNA sequencing (snRNA-seq) identified five molecularly distinct neuronal subtypes in mouse brain. Additionally, multiplex fluorescent situ hybridization top marker genes revealed that are organized by a combination previously unidentified gradients. Lastly, comparing our dataset with recently published single-cell atlas yielded novel insight into PVT’s connectivity cortex, including unexpected innervation auditory visual areas. This comparison also data contains largely non-overlapping transcriptomic map multiple midline thalamic nuclei. Collectively, findings uncover unknown features anatomical organization provide valuable resource for future investigations.

Language: Английский

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A common thalamic hub for general and defensive arousal control

Neuron, Journal Year: 2023, Volume and Issue: 111(20), P. 3270 - 3287.e8

Published: Aug. 8, 2023

Language: Английский

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Dissociable encoding of motivated behavior by parallel thalamo-striatal projections

Current Biology, Journal Year: 2024, Volume and Issue: 34(7), P. 1549 - 1560.e3

Published: March 7, 2024

The successful pursuit of goals requires the coordinated execution and termination actions that lead to positive outcomes. This process relies on motivational states are guided by internal drivers, such as hunger or fear. However, mechanisms which brain tracks shape instrumental not fully understood. paraventricular nucleus thalamus (PVT) is a midline thalamic shapes motivated behaviors via its projections accumbens (NAc)

Language: Английский

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The nucleus accumbens in reward and aversion processing: insights and implications

Frontiers in Behavioral Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Aug. 9, 2024

The nucleus accumbens (NAc), a central component of the brain's reward circuitry, has been implicated in wide range behaviors and emotional states. Emerging evidence, primarily drawing from recent rodent studies, suggests that function NAc aversion processing is multifaceted. Prolonged stress or drug use induces maladaptive neuronal which results pathological conditions. This review aims to provide comprehensive up-to-date insights on role motivated behavior regulation highlights areas demand further in-depth analysis. It synthesizes latest findings how distinct populations pathways contribute opposite valences. examines neuromodulators, especially monoamines, influence NAc's control over various motivational Furthermore, it delves into complex underlying mechanisms psychiatric disorders such as addiction depression evaluates prospective interventions restore functionality.

Language: Английский

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Distinct Behavioral Profiles and Neuronal Correlates of Heroin Vulnerability Versus Resiliency in a Multi-Symptomatic Model of Heroin Use Disorder in Rats

American Journal of Psychiatry, Journal Year: 2025, Volume and Issue: 182(2), P. 198 - 208

Published: Jan. 15, 2025

The behavioral and diagnostic heterogeneity within the opioid use disorder (OUD) diagnosis is not readily captured in current animal models, limiting translational relevance of mechanistic research that conducted experimental animals. authors hypothesized a nonlinear clustering OUD-like traits would capture population yield subpopulations OUD vulnerable rats with distinct neurocircuit profiles. Over 900 male female heterogeneous stock rats, line capturing genetic present humans, were assessed for several measures heroin rewarded non-rewarded seeking behaviors. A stochastic block model analysis was used to assign vulnerable, intermediate, resilient clusters. Additional tests circuit analyses using c-fos protein activation on subpopulations. exhibited greater taking behaviors relative those intermediate Akin human diagnosis, further rat subclustering revealed different combinations traits, including sex differences. Lastly, cue-induced neuronal patterns differed between phenotypes. Behavioral differences recapitulated circuitry activation, preferential engagement extended amygdala stress males cortico-striatal drug cue-seeking females. Using approach reflective diagnosis. vulnerability resiliency associated patterns, posing this as tool assessing neurobiological mechanisms underpinning OUD.

Language: Английский

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Treadmill exercise prevents stress-induced anxiety-like behaviors via enhancing the excitatory input from the primary motor cortex to the thalamocortical circuit

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 22, 2025

Physical exercise effectively prevents anxiety disorders caused by environmental stress. The neural circuitry mechanism, however, remains incomplete. Here, we identified a previously unrecognized pathway originating from the primary motor cortex (M1) to medial prefrontal (mPFC) via ventromedial thalamic (VM) nuclei in male mice. Besides anatomical evidence, both ex vivo and recordings showed enhanced excitability of M1-VM inputs prelimbic (PrL) region mPFC upon 14-day treadmill on chronic restraint stress (CRS) mouse model. Further functional interrogations demonstrated that activation this circuit is necessary sufficient direct anxiolytic effect training CRS Our findings provide more insights into circuits connecting mental regions under paradigm implicate potential targets for neuromodulation treating disorders. relieves disorders, while authors show input cortex, nuclei.

Language: Английский

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