The STRIPAK component SipC is involved in morphology and cell-fate determination in the nematode-trapping fungus Duddingtonia flagrans

Genetics, Journal Year: 2021, Volume and Issue: 220(1)

Published: Sept. 25, 2021

Abstract The striatin-interacting phosphatase and kinase (STRIPAK) complex is a highly conserved eukaryotic signaling hub involved in the regulation of many cellular processes. In filamentous fungi, STRIPAK controls multicellular development, hyphal fusion, septation, pathogenicity. this study, we analyzed role nematode-trapping fungus Duddingtonia flagrans which forms three-dimensional, adhesive trapping networks to capture Caenorhabditis elegans. Trap consist several loops are morphologically functionally different from vegetative hyphae. We show that lack component SipC (STRIP1/2/HAM-2/PRO22) results incomplete loop formation column-like trap structures with elongated compartments. misshapen or traps lost their identity continued growth as same effect was observed presence actin cytoskeleton drug cytochalasin A. These could suggest link between functions.

Language: Английский

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The small-secreted cysteine-rich protein CyrA is a virulence factor participating in the attack of Caenorhabditis elegans by Duddingtonia flagrans

PLoS Pathogens, Journal Year: 2021, Volume and Issue: 17(11), P. e1010028 - e1010028

Published: Nov. 4, 2021

Nematode-trapping fungi (NTF) are a diverse and intriguing group of that live saprotrophically but can switch to predatory lifestyle when starving in the presence nematodes. NTF like Arthrobotrys oligospora or Duddingtonia flagrans produce adhesive trapping networks catch immobilize After penetration cuticle, hyphae grow develop inside worm secrete large amounts hydrolytic enzymes for digestion. In many microbial pathogenic interactions small-secreted proteins (SSPs) used manipulate host. The genome D . encodes more than 100 such putative SSPs one which is cy steine- r ich protein CyrA. We have chosen this gene further analysis because it only found appeared be upregulated during interaction. show cyrA was transcriptionally induced trap cells, accumulated at inner rim hyphal ring before Caenorhabditis elegans capture. penetration, fungal infection bulb, where likely secreted with help exocyst complex. A -deletion strain less virulent, time from capture paralysis extended. Heterologous expression CyrA C reduced its lifespan. coelomocytes possibly inactivated. This first example may important interactions.

Language: Английский

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Synchronization of oscillatory growth prepares fungal hyphae for fusion

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Aug. 21, 2023

Communication is crucial for organismic interactions, from bacteria, to fungi, humans. Humans may use the visual sense monitor environment before starting acoustic interactions. In comparison, lacking a system, rely on cell-to-cell dialogue based secreted signaling molecules coordinate cell fusion and establish hyphal networks. Within this dialogue, hyphae alternate between sending receiving signals. This pattern can be visualized via putative protein Soft (SofT), mitogen-activated kinase MAK-2 (MakB) which are recruited in an alternating oscillatory manner respective cytoplasmic membrane or nuclei of interacting hyphae. Here, we show that signal oscillations already occur single Arthrobotrys flagrans absence potential partners (cell monologue). They were same phase as growth oscillations. contrast anti-phasic observed during SofT MakB displayed synchronized monologue. Once two came into each other's vicinity, their oscillation frequencies slowed down (entrainment phase) transit synchronization cells' with 104±28 s 117±19 s, respectively. Single-cell oscillations, transient entrainment, reproduced by mathematical model where nearby absorb secrete limited molecular component shared extracellular space. We intracellular Ca2+ concentrations oscillate approaching hyphae, depletion medium affected vesicle-driven extension tip, abolished monologue Our results suggest engage 'monologue' used exploration dynamically shift systems 'dialogue' initiate fusion.

Language: Английский

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Cellular communication and fusion regulate cell fusion, trap morphogenesis, conidiation, and secondary metabolism in Arthrobotrys oligospora

Microbiological Research, Journal Year: 2023, Volume and Issue: 278, P. 127516 - 127516

Published: Oct. 12, 2023

Language: Английский

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Establishment of Arthrobotrys flagrans as biocontrol agent against the root pathogenic nematode Xiphinema index

Environmental Microbiology, Journal Year: 2022, Volume and Issue: 25(2), P. 283 - 293

Published: Nov. 10, 2022

Plant-parasitic nematodes cause devastating agricultural damage worldwide. Only a few synthetic nematicides can be used and their application is limited in fields. Therefore, there need for sustainable environment-friendly alternatives. Nematode-trapping fungi (NTF) are natural predators of nematodes. They capture digest them with hyphae starting to being as bio-control agents. In this study, we applied the NTF Arthrobotrys flagrans (Duddingtonia flagrans) against wine pathogenic nematode Xiphinema index. A. reduced number X. index juveniles pot cultures Ficus carica, an alternative host plant index, significantly. Sodium-alginate pellets spores were produced vineyard soil inoculation under laboratory conditions. The conoides, musiformis superba enriched from several samples, showing presence. Trap formation energy-consuming process depends upon various biotic abiotic stimuli. Here, show that bacteria genus Delftia, Bacillus, Pseudomonas, Enterobacter Serratia induced trap like conoides oligospora but not absence along such could combinatorial way efficient biocontrol nematode-infested soil.

Language: Английский

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