Modulating Neutrophil Extracellular Trap Formation In Vivo with Locoregional Precision Using Differently Charged Self-Assembled Hydrogels

ACS Central Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Neutrophil extracellular traps (NETs) are DNA networks released by neutrophils, first described as a defense response against pathogens but have since been associated with numerous inflammatory diseases. Diverse physical material properties shown to promote NET formation. Herein, we report the discovery that charge of self-assembled peptide hydrogels predictably modulates formation NETs in vivo within implanted material. Positively charged gels induce rapid release, whereas negatively do not. This differential immune our enabled development platform allows rheostat-like modulation over degree anatomical and locoregional control.

Language: Английский

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A sandcastle worm-inspired strategy to functionalize wet hydrogels

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Nov. 3, 2021

Abstract Hydrogels have been extensively used in many fields. Current synthesis of functional hydrogels requires incorporation molecules either before or during gelation via the pre-organized reactive site along polymer chains within hydrogels, which is tedious for and not flexible different types hydrogels. Inspired by sandcastle worm, we develop a simple one-step strategy to functionalize wet using bearing an adhesive dibutylamine-DOPA-lysine-DOPA tripeptide. This tripeptide can be easily modified with various groups initiate diverse polymerizations provide polymers terminal Such enable direct modification acquire biological functions such as antimicrobial, cell adhesion wound repair. The has tunable functionalization degree stable attachment molecules, provides tool convenient them applications.

Language: Английский

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Soft robot–mediated autonomous adaptation to fibrotic capsule formation for improved drug delivery

Science Robotics, Journal Year: 2023, Volume and Issue: 8(81)

Published: Aug. 30, 2023

The foreign body response impedes the function and longevity of implantable drug delivery devices. As a dense fibrotic capsule forms, integration device with host tissue becomes compromised, ultimately resulting in seclusion treatment failure. We present FibroSensing Dynamic Soft Reservoir (FSDSR), an capable monitoring formation overcoming its effects via soft robotic actuations. Occlusion FSDSR porous membrane was monitored over 7 days rodent model using electrochemical impedance spectroscopy. electrical resistance correlated to increase thickness volume. Our showed great sensitivity detecting changes at abiotic/biotic interface, such as collagen deposition myofibroblast proliferation. potential overcome maintain constant dosing time demonstrated silico vitro. Controlled closed loop release methylene blue into agarose gels (with comparable fold change permeability relating 28 vivo) achieved by adjusting magnitude frequency pneumatic actuations after measurements membrane. By sensing vivo, will be probing adapting through dynamic actuation changes. Informed real-time sensor signals, this offers for long-term efficacy sustained dosing, even setting formation.

Language: Английский

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Week-long normoglycaemia in diabetic mice and minipigs via a subcutaneous dose of a glucose-responsive insulin complex

Nature Biomedical Engineering, Journal Year: 2023, Volume and Issue: 8(10), P. 1214 - 1225

Published: Dec. 6, 2023

Language: Английский

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Robust, stretchable bioelectronic interfaces for cardiac pacing enabled by interfacial transfer of laser-induced graphene via water-response, nonswellable PVA gels

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 261, P. 116453 - 116453

Published: May 28, 2024

Language: Английский

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