Small Methods,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 26, 2025
Fluorescence
imaging
has
been
a
powerful
technique
for
the
visualization
of
interest
biomolecules.
It
is
widely
used
in
basic
biological
research,
clinical
medicine,
and
other
fields.
However,
fluorescence
signals
are
often
too
weak
detecting
with
simple
devices
or
complex
environments.
Besides,
limited
to
about
four
six
dyes,
restricting
spectral
overlap
organic
fluorophores.
DNA
nanotechnology
including
structured
dynamic
emerges
as
promising
material
encode
fluorophores,
holding
great
potential
improve
properties
signals.
Substantial
progresses
have
achieved
DNA-encoded
signals,
exhibiting
novel
characteristics
applications.
This
review
summarizes
various
encoding
strategies
their
performance
analysis.
In
this
review,
different
methods
impacts
on
reported,
such
brightness,
photostability,
kinetics,
multiplexing.
application
reviewed.
Finally,
solutions
address
current
challenges
suggested,
encouraging
future
development
area.
Journal of Biomedical Science,
Journal Year:
2022,
Volume and Issue:
29(1)
Published: Nov. 14, 2022
Abstract
In
the
past
decade,
single-cell
technologies
have
revealed
heterogeneity
of
tumor-immune
microenvironment
at
genomic,
transcriptomic,
and
proteomic
levels
furthered
our
understanding
mechanisms
tumor
development.
Single-cell
also
been
used
to
identify
potential
biomarkers.
However,
spatial
information
about
such
as
cell
locations
cell–cell
interactomes
is
lost
in
these
approaches.
Recently,
multi-omics
study
transcriptomes,
proteomes,
metabolomes
microenvironments
several
types
cancer,
data
obtained
from
methods
has
combined
with
immunohistochemistry
multiparameter
analysis
yield
markers
cancer
progression.
Here,
we
review
numerous
cutting-edge
‘omics
techniques,
their
application
microenvironment,
remaining
technical
challenges.
Genome biology,
Journal Year:
2022,
Volume and Issue:
23(1)
Published: Dec. 13, 2022
Abstract
Spatial
omics
technologies
enable
a
deeper
understanding
of
cellular
organizations
and
interactions
within
tissue
interest.
These
assays
can
identify
specific
compartments
or
regions
in
with
differential
transcript
protein
abundance,
delineate
their
interactions,
complement
other
methods
defining
phenotypes.
A
variety
spatial
methodologies
are
being
developed
commercialized;
however,
these
techniques
differ
resolution,
multiplexing
capability,
scale/throughput,
coverage.
Here,
we
review
the
current
prospective
landscape
single
cell
to
subcellular
resolution
analysis
tools
provide
comprehensive
picture
for
both
research
clinical
applications.
Journal of genetics and genomics/Journal of Genetics and Genomics,
Journal Year:
2023,
Volume and Issue:
50(9), P. 625 - 640
Published: March 27, 2023
The
ability
to
explore
life
kingdoms
is
largely
driven
by
innovations
and
breakthroughs
in
technology,
from
the
invention
of
microscope
350
years
ago
recent
emergence
single-cell
sequencing,
which
scientific
community
has
been
able
visualize
at
an
unprecedented
resolution.
Most
recently,
Spatially
Resolved
Transcriptomics
(SRT)
technologies
have
filled
gap
probing
spatial
or
even
three-dimensional
organization
molecular
foundation
behind
mysteries
life,
including
origin
different
cellular
populations
developed
totipotent
cells
human
diseases.
In
this
review,
we
introduce
progress
challenges
on
SRT
perspectives
bioinformatic
tools,
as
well
representative
applications.
With
currently
fast-moving
promising
results
early
adopted
research
projects,
can
foresee
bright
future
such
new
tools
understanding
most
profound
analytical
level.
Chemical Reviews,
Journal Year:
2023,
Volume and Issue:
124(2), P. 554 - 628
Published: Nov. 22, 2023
In
vivo
imaging
technologies
have
emerged
as
a
powerful
tool
for
both
fundamental
research
and
clinical
practice.
particular,
luminescence
in
the
tissue-transparent
near-infrared
(NIR,
700–1700
nm)
region
offers
tremendous
potential
visualizing
biological
architectures
pathophysiological
events
living
subjects
with
deep
tissue
penetration
high
contrast
owing
to
reduced
light–tissue
interactions
of
absorption,
scattering,
autofluorescence.
The
distinctive
quantum
effects
nanocrystals
been
harnessed
achieve
exceptional
photophysical
properties,
establishing
them
promising
category
luminescent
probes.
this
comprehensive
review,
between
light
tissues,
well
advantages
NIR
imaging,
are
initially
elaborated.
Subsequently,
we
focus
on
achieving
improved
by
optimizing
performance
nanocrystal
fluorophores.
ingenious
design
strategies
probes
discussed,
along
their
respective
biomedical
applications
versatile
modalities.
Finally,
thought-provoking
reflections
challenges
prospects
future
translation
nanocrystal-based
wisely
provided.
Frontiers in Oncology,
Journal Year:
2022,
Volume and Issue:
12
Published: Oct. 13, 2022
In
recent
years,
spatial
transcriptomics
(ST)
technologies
have
developed
rapidly
and
been
widely
used
in
constructing
tissue
atlases
characterizing
spatiotemporal
heterogeneity
of
cancers.
Currently,
ST
has
to
profile
multiple
cancer
types.
Besides,
is
a
benefit
for
identifying
comprehensively
understanding
special
areas
such
as
tumor
interface
tertiary
lymphoid
structures
(TLSs),
which
exhibit
unique
microenvironments
(TMEs).
Therefore,
also
shown
great
potential
improve
pathological
diagnosis
identify
novel
prognostic
factors
cancer.
This
review
presents
advances
prospects
applications
on
research
based
well
the
challenges.
Cancers,
Journal Year:
2024,
Volume and Issue:
16(3), P. 480 - 480
Published: Jan. 23, 2024
Colorectal
cancer
(CRC)
is
one
of
the
most
heterogeneous
and
deadly
diseases,
with
a
global
incidence
1.5
million
cases
per
year.
Genomics
has
revolutionized
clinical
management
CRC
by
enabling
comprehensive
molecular
profiling
cancer.
However,
deeper
understanding
factors
needed
to
identify
new
prognostic
predictive
markers
that
can
assist
in
designing
more
effective
therapeutic
regimens
for
improved
CRC.
Recent
breakthroughs
single-cell
analysis
have
identified
cell
subtypes
play
critical
role
tumor
progression
could
serve
as
potential
targets.
Spatial
transcriptome
proteome
holds
key
unlocking
pathogenic
cellular
interactions,
while
liquid
biopsy
variables
from
serum
great
monitoring
therapy
resistance.
Furthermore,
gene
expression
signatures
various
pathways
emerged
promising
indicators
colorectal
enhance
development
equitable
medicine.
The
advancement
these
technologies
identifying
markers,
particularly
domain
personalized
medicine,
improve
patients
Further
investigations
utilizing
similar
methods
uncover
specific
emerging
therapies,
potentially
strengthening
medicine
patients.
Small Methods,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 18, 2025
Abstract
Rapidly
developing
spatial
omics
technologies
provide
us
with
new
approaches
to
deeply
understanding
the
diversity
and
functions
of
cell
types
within
organisms.
Unlike
traditional
approaches,
enable
researchers
dissect
complex
relationships
between
tissue
structure
function
at
cellular
or
even
subcellular
level.
The
application
provides
perspectives
on
key
biological
processes
such
as
nervous
system
development,
organ
tumor
microenvironment.
This
review
focuses
advancements
strategies
technologies,
summarizes
their
applications
in
biomedical
research,
highlights
power
advancing
life
sciences
related
development
disease.
