Angewandte Chemie,
Journal Year:
2023,
Volume and Issue:
136(11)
Published: Dec. 12, 2023
Abstract
Organic
fluorophores
are
indispensable
tools
in
cells,
tissue
and
vivo
imaging,
have
enabled
much
progress
the
wide
range
of
biological
biomedical
fields.
However,
many
available
dyes
suffer
from
insufficient
performances,
such
as
short
absorption
emission
wavelength,
low
brightness,
poor
stability,
small
Stokes
shift,
unsuitable
permeability,
restricting
their
application
advanced
imaging
technology
complex
imaging.
Over
past
two
decades,
efforts
been
made
to
improve
these
performances
fluorophores.
Starting
with
luminescence
principle
fluorophores,
this
review
clarifies
mechanisms
performance
for
traditional
a
certain
extent,
systematically
summarizes
modified
approaches
optimizing
properties,
highlights
typical
applications
improved
sensing,
indicates
existing
problems
challenges
area.
This
not
only
proves
significance
improving
but
also
provide
theoretical
guidance
development
high‐performance
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(16)
Published: Feb. 27, 2024
Abnormal
physiological
processes
and
diseases
can
lead
to
content
or
activity
fluctuations
of
biocomponents
in
organelles
whole
blood.
However,
precise
monitoring
these
abnormalities
remains
extremely
challenging
due
the
insufficient
sensitivity
accuracy
available
fluorescence
probes,
which
be
attributed
background
arising
from
two
sources,
1)
biocomponent
autofluorescence
(BCAF)
2)
probe
intrinsic
(PIF).
To
overcome
obstacles,
we
have
re-engineered
far-red
NIR
II
rhodol
derivatives
that
possess
weak
BCAF
interference.
And
a
series
"zero"
PIF
sensing-platforms
were
created
by
systematically
regulating
open-loop/spirocyclic
forms.
Leveraging
advancements,
devised
various
ultra-sensitive
indicators,
achieving
substantial
boosts
(190
1300-fold).
Among
8-LAP
demonstrated
accurate
tracking
quantifying
leucine
aminopeptidase
(LAP)
blood
at
stages
tumor
metastasis.
Furthermore,
coupling
with
an
endoplasmic
reticulum-targeting
element
enabled
detection
ERAP1
HCT116
cells
p53
abnormalities.
This
delicate
design
eliminating
provides
insights
into
enhancing
existing
probes
toward
imaging
abnormal
diseases.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(17), P. 10097 - 10105
Published: April 17, 2024
With
the
booming
development
of
food
manufacturing,
developing
ideal
analytical
tools
to
precisely
quantify
additives
is
highly
sought
after
in
science
field.
Herein,
a
new
series
quinoline-derived
multifunctional
fluorescent
probes
has
been
synthesized.
Bearing
double
reactive
sites,
these
compounds
display
fluorescence
response
toward
both
bisulfite
(HSO
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 12, 2024
Chemigenetic
fusion
of
synthetic
dyes
with
genetically
encoded
protein
tags
presents
a
promising
avenue
for
in
vivo
imaging.
However,
its
full
potential
has
been
hindered
by
the
lack
bright
and
fluorogenic
operating
"tissue
transparency"
near-infrared
window
(NIR,
700–1700
nm).
Here,
we
report
2X-rhodamine
(2XR),
novel
scaffold
that
allows
development
live-cell-compatible,
NIR-excited
variants
strong
fluorogenicity
beyond
1000
nm.
2XR
utilizes
rigidified
π-skeleton
featuring
dual
atomic
bridges
functions
via
spiro-based
mechanism.
This
design
affords
longer
wavelengths,
higher
quantum
yield
(ΦF
=
0.11),
enhanced
water
when
compared
to
phosphine
oxide-cored,
or
sulfone-cored
rhodamine,
NIR
benchmarks
currently
used.
We
showcase
their
performance
video-rate
dynamic
imaging
targeted
deep-tissue
molecular
vivo.
Notably,
develop
variant,
2XR715-HTL,
an
ligand
HaloTag
protein,
enabling
calcium
sensing
first
demonstration
chemigenetic
labeling
Our
work
expands
library
tools,
paving
way
approach.
Angewandte Chemie,
Journal Year:
2023,
Volume and Issue:
136(11)
Published: Dec. 12, 2023
Abstract
Organic
fluorophores
are
indispensable
tools
in
cells,
tissue
and
vivo
imaging,
have
enabled
much
progress
the
wide
range
of
biological
biomedical
fields.
However,
many
available
dyes
suffer
from
insufficient
performances,
such
as
short
absorption
emission
wavelength,
low
brightness,
poor
stability,
small
Stokes
shift,
unsuitable
permeability,
restricting
their
application
advanced
imaging
technology
complex
imaging.
Over
past
two
decades,
efforts
been
made
to
improve
these
performances
fluorophores.
Starting
with
luminescence
principle
fluorophores,
this
review
clarifies
mechanisms
performance
for
traditional
a
certain
extent,
systematically
summarizes
modified
approaches
optimizing
properties,
highlights
typical
applications
improved
sensing,
indicates
existing
problems
challenges
area.
This
not
only
proves
significance
improving
but
also
provide
theoretical
guidance
development
high‐performance
