Probing nanomechanical interactions of SARS-CoV-2 variants Omicron and XBB with common surfaces DOI
Yuelong Xiao, Bin Zheng, Xuan Ding

et al.

Chemical Communications, Journal Year: 2023, Volume and Issue: 59(75), P. 11268 - 11271

Published: Jan. 1, 2023

The emergence of SARS-CoV-2 variants has further raised concerns about viral transmission. A fundamental understanding the intermolecular interactions between coronavirus and different surfaces is needed to address transmission through respiratory droplet-contaminated or fomites. receptor-binding domain (RBD) spike protein a key target for adhesion on surface. To understand effect mutations adhesion, atomic force microscopy-based single-molecule spectroscopy (AFM-SMFS) was used quantify wild-type, Omicron, XBB with several surfaces. measurement revealed that RBD exhibits relatively higher forces paper gold surfaces, average being 1.5 times greater compared plastic In addition, elevation can reach ∼28% relative wild type. These findings enhance our nanomechanical virus common

Language: Английский

The nervous system during COVID‐19: Caught in the crossfire DOI Creative Commons
Nick R. Natale, John R. Lukens, William A. Petri

et al.

Immunological Reviews, Journal Year: 2022, Volume and Issue: 311(1), P. 90 - 111

Published: June 30, 2022

SUMMARY SARS‐CoV‐2, the virus that causes coronavirus disease (COVID)‐19, has become a persistent global health threat. Individuals who are symptomatic for COVID‐19 frequently exhibit respiratory illness, which is often accompanied by neurological symptoms of anosmia and fatigue. Mounting clinical data also indicate many patients display long‐term disorders postinfection such as cognitive decline, emphasizes need to further elucidate effects on central nervous system. In this review article, we summarize an emerging body literature describing impact SARS‐CoV‐2 infection system (CNS) highlight important areas future investigation.

Language: Английский

Citations

15
Multivalent acetylated-sialic acid as recognition elements for the electrochemical sensing of viral antigens DOI
Zhen Zhang,

Haijie Ji,

Xiwei Zhuang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 268, P. 116883 - 116883

Published: Oct. 30, 2024

Language: Английский

Citations

3
Probing living cell dynamics and molecular interactions using atomic force microscopy DOI
David Alsteens

Biophysical Reviews, Journal Year: 2024, Volume and Issue: 16(6), P. 663 - 677

Published: Dec. 1, 2024

Language: Английский

Citations

3
Mechanosensing view of SARS-CoV-2 infection by a DNA nano-assembly DOI Creative Commons
Jialü Zhang,

Yihao Huang,

Miao Sun

et al.

Cell Reports Physical Science, Journal Year: 2022, Volume and Issue: 3(9), P. 101048 - 101048

Published: Sept. 1, 2022

The mechanical force between a virus and its host cell plays critical role in viral infection. However, characterization of the virus-cell at whole-virus level remains challenge. Herein, we develop platform which is anchored with multivalence-controlled aptamers to achieve transfer DNA tension gauge tether (Virus-TGT). When TGT ruptured, complex binding module-virus-cell detached from substrate, accompanied by decreased cell-substrate adhesion, thus revealing cell. Using Virus-TGT, direct evidence about biomechanical SARS-CoV-2 obtained. relative gap (<10 pN) cellular wild-type variant measured, suggesting possible positive correlation infectivity. Overall, this strategy provides new perspective probe force.

Language: Английский

Citations

13
Nine-valent oleanolic acid conjugates as potent inhibitors blocking the entry of influenza A virus DOI
Liang Shao,

Yangqing Su,

Yuan Zhang

et al.

European Journal of Medicinal Chemistry, Journal Year: 2023, Volume and Issue: 258, P. 115562 - 115562

Published: June 10, 2023

Language: Английский

Citations

8
Recent experimental advances in probing the colloidal properties of viruses DOI Creative Commons
Antonius Armanious, Milad Radiom, Raffaele Mezzenga

et al.

Current Opinion in Colloid & Interface Science, Journal Year: 2023, Volume and Issue: 66, P. 101703 - 101703

Published: May 10, 2023

Colloidal properties of viruses largely define the stability, transport, and host interactions viruses. Despite attempts to unravel correlation between colloidal virus their outside inside host, an in-depth understanding is still missing. This knowledge gap is, a great extent, caused by challenges associated with capacity probe these experimentally; thus, efforts are being invested in developing new approaches or transforming existing ones characterize physical-chemical, i.e., colloidal, Understanding not only important from scientific point view but will also hopefully inspire design novel viral vectors virus-like particles for biomedical applications. In this review, we cover recent experimental advances characterizing particular attention hydrophobicity, genetic load, nanomechanical properties, surface interaction forces cells.

Language: Английский

Citations

8
Deep Learning Image Recognition-Assisted Atomic Force Microscopy for Single-Cell Efficient Mechanics in Co-culture Environments DOI

Xuliang Yang,

Yanqi Yang,

Zhihui Zhang

et al.

Langmuir, Journal Year: 2023, Volume and Issue: 40(1), P. 837 - 852

Published: Dec. 28, 2023

Atomic force microscopy (AFM)-based spectroscopy assay has become an important method for characterizing the mechanical properties of single living cells under aqueous conditions, but a disadvantage is its reliance on manual operation and experience as well resulting low throughput. Particularly, providing capacity to accurately identify type cell grown in co-culture environments without need fluorescent labeling will further facilitate applications AFM life sciences. Here, we present study deep learning image recognition-assisted AFM, which not only enables fluorescence-independent recognition identity co-cultured also allows efficient downstream measurements identified cells. With use learning-based model, viability individual were directly from optical bright-field images, confirmed by following growth results. Based results, positional relationship between probe targeted was automatically determined, allowing precise movement target perform measurements. The experimental results show that presented applicable conventional (microsphere-modified) used indentation measuring Young's modulus single-cell AFM-based (SCFS) adhesion forces illustrates imaging promising approach label-free high-throughput detection mechanics unraveling cues involved cell–cell interactions their native states at level benefit field mechanobiology.

Language: Английский

Citations

7
Suzuki‐Miyaura Reaction of Glycals with Base‐Labile Protecting Groups as a New Route to Glycoporphyrins DOI
Bartosz Godlewski, Maciej Malinowski

Advanced Synthesis & Catalysis, Journal Year: 2024, Volume and Issue: 366(13), P. 2956 - 2966

Published: May 22, 2024

Abstract Glycoporphyrins are considered promising photosensitisers of new generation. Herein, we describe a metal‐catalysed synthetic method towards porphyrin‐carbohydrate C−C conjugates. The protocol Suzuki‐Miyaura reaction has been developed and it was compatible with peracetylated 2‐iodoglycals. utility the exemplified on 15 different (C‐2)‐arylated sugar derivatives. Then applied more challenging starting materials, namely, porphyrin boronates in which addressed issues difficult transmetalation step usually observed palladium‐catalysed processes efficiency finally to synthesize library glycoporphyrins proving versatile character this strategy. Finally, methodology transferred onto A 4 ‐type efficiently obtain an interesting example multivalent glycoconjugate.

Language: Английский

Citations

2
The Glycan-Binding Trait of the Sarbecovirus Spike N-Terminal Domain Reveals an Evolutionary Footprint DOI
Hua Guo, Ang Li,

Haofeng Lin

et al.

Journal of Virology, Journal Year: 2022, Volume and Issue: 96(15)

Published: July 19, 2022

The spike protein on sarbecovirus virions contains two external, protruding domains: an N-terminal domain (NTD) with unclear function and a C-terminal (CTD) that binds the host receptor, allowing for viral entry infection. While CTD is well studied therapeutic interventions, role of NTD far less understood many coronaviruses. Here, we demonstrate from SARS-CoV-2 other sarbecoviruses to unidentified glycans

Language: Английский

Citations

11
Pathogen-host adhesion between SARS-CoV-2 spike proteins from different variants and human ACE2 studied at single-molecule and single-cell levels DOI Creative Commons
Xiaoxu Zhang,

Bixia Hong,

Peng Wei

et al.

Emerging Microbes & Infections, Journal Year: 2022, Volume and Issue: 11(1), P. 2658 - 2669

Published: Sept. 25, 2022

The binding of the receptor domain (RBD) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein onto human angiotensin-converting enzyme (ACE2) is considered as first step for virus to adhere host cells during infection. Here, we investigated adhesion proteins from different variants and ACE2 using single-molecule single-cell force spectroscopy. We found that unbinding probability Delta variant were highest among tested in our study at both levels. As most popular variants, Omicron have slightly higher than wild type. Molecular dynamics simulation showed ACE2-RBD (Omicron BA.1) complex destabilized by E484A Y505H mutations stabilized S477N N501Y mutations, when compared with variant. In addition, a neutralizing antibody, produced immunization type protein, could effectively inhibit type, (BA.1 BA.5) ACE2. Our results provide new insight molecular mechanism adhesive interactions between suggest effective monoclonal antibody can be prepared against variants.

Language: Английский

Citations

11