Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 27, 2024

Abstract In 2015, we launched the mesoSPIM initiative, an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, demand imaging larger samples at higher speed and resolution has increased, requiring major improvements in capabilities such microscopes. Here, introduce next-generation (“Benchtop”) with a significantly increased field view, improved resolution, throughput, affordable cost, simpler assembly compared to original version. We develop optical method testing detection objectives that enables us select optimal large-sensor cameras. The achieves high spatial (1.5 µm laterally, 3.3 axially) across entire magnification up 20×, supports sample sizes ranging from sub-mm several centimeters while being compatible multiple clearing techniques. microscope serves broad range applications neuroscience, developmental biology, pathology, even physics.

Language: Английский

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Crusaders, monks and family fortunes: evolutionary models of male homosexuality and related phenomena

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2025, Volume and Issue: 292(2042)

Published: March 1, 2025

The fraternal birth order effect (FBOE) is a well documented preponderance of older brothers among gay men. Until now, it has lacked proper formal treatment in light evolutionary theory. In this article, we propose two related mathematical models based on the rigorous application inclusive fitness concept. examine sibling rivalry and resource allocation within families. One assumes that laterborn's reproductive ability not compromised by manipulation leads to exclusive family resources firstborn. other model posits may lower direct zero. Both suggest FBOE arises primarily from brother's manipulation, as firstborn's increase, while mother's decreases, but vice versa. However, under certain conditions, mother should support an activity discourages division resources, extreme cases, younger homosexuality be best available strategy for his genes. also provide insight into cultural evolution primogeniture explain how between relatives could evolve before emergence substantial economic inequality.

Language: Английский

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Olfactory bulb activity shapes the development of entorhinal-hippocampal coupling and associated cognitive abilities

Current Biology, Journal Year: 2023, Volume and Issue: 33(20), P. 4353 - 4366.e5

Published: Sept. 19, 2023

The interplay between olfaction and higher cognitive processing has been documented in the adult brain; however, its development is poorly understood. In mice, shortly after birth, endogenous stimulus-evoked activity olfactory bulb (OB) boosts oscillatory entrainment of downstream lateral entorhinal cortex (LEC) hippocampus (HP). However, it unclear whether early OB a long-lasting impact on entorhinal-hippocampal function processing. Here, we chemogenetically silenced synaptic outputs mitral/tufted cells, main projection neurons OB, during postnatal days 8-10. transient manipulation leads to reduction coupling weaker responsiveness stimuli within developing circuits accompanied by dendritic sparsification LEC pyramidal neurons. Moreover, silencing reduces performance behavioral tests involving later life. Thus, neonatal critical for functional LEC-HP maturation abilities.

Language: Английский

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Immune control of brain physiology

Nature reviews. Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Language: Английский

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Micro-to multi-chimerism: the multiple facets of a singular phenomenon

Seminars in Immunopathology, Journal Year: 2025, Volume and Issue: 47(1)

Published: Feb. 18, 2025

Language: Английский

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Analysis of SMN protein in umbilical cord blood and postnatal peripheral blood of neonates with SMA: a rationale for prompt treatment initiation to prevent SMA development

Orphanet Journal of Rare Diseases, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 28, 2025

Abstract Background Spinal muscular atrophy (SMA) is a severe genetic neuromuscular disease caused by insufficient functional survival motor neuron protein (SMN). The SMN expression level in the spinal cord highest during 2nd trimester of foetal period. We previously reported spot analysis peripheral blood using imaging flow cytometry (IFC) as biomarker expression. In this study, we analysed neonatal blood, postnatal and maternal presymptomatic five infants whose sibling has type 1 SMA to estimate prenatal dynamics before onset SMA. Results Data from 37 untreated patients with showed that SMN-spot + cells were significantly correlated clinical classification copy numbers SMN2 gene. range values for converted each number statistics, was − 0.7 2.0 standard deviation (SD) (0.1–24.0%) Subsequent analyses neonates ranged 0.8 SD (0.4–15.2%). carrier 0.2 2.4 (1.4–25.2%). A correlation observed between blood. Conclusions This study suggests status pool can be presumed depletion determines timing onset. As tended decrease time after birth, they may eventually lead development Furthermore, found which predicts severity birth. other words, supplied mother foetus suppress infant at occur causing develop Our findings demonstrated effectiveness newborn screening potential maternally mediated treatment strategies providing rationale prompt initiation

Language: Английский

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Maternal microchimeric cell trafficking and its biological consequences depend on the onset of inflammation at the feto-maternal interface

Seminars in Immunopathology, Journal Year: 2025, Volume and Issue: 47(1)

Published: Jan. 17, 2025

Abstract Microchimerism is defined as the presence of a small population genetically distinct cells within host that derived from another individual. Throughout pregnancy, maternal and fetal are known to traffic across feto-maternal interface result in microchimerism, respectively. However, routes cell transfer, molecular signaling well timing which trafficking takes place still not completely understood. Recently, inflammation at has been linked with microchimeric modulating organ development fetus. Here, we review current literature suggest inflammatory processes tissues physiological prerequisite for establishment microchimerism. We further propose spatio-temporal corridor migration potentially explain some biological effects Additionally, elaborate on possible consequences shift this corridor, responsible pathologies neonate.

Language: Английский

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Assessing in-vitro models for microglial development and fetal programming: a critical review

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 29, 2025

This review evaluates in-vitro models for studying how maternal influences during pregnancy impact the development of offspring microglia, immune cells central nervous system. The examined include primary microglia cultures, cell lines, iPSC-derived PBMC-induced microglia-like cells, 3D brain organoids derived from iPSCs, and Hofbauer cells. Each model is assessed its ability to replicate in-vivo environment developing brain, with a focus on their strengths, limitations, practical challenges. Key factors such as scalability, genetic epigenetic fidelity, physiological relevance are highlighted. Microglia lines highly scalable but lack fidelity. provide moderate patient-specific insights face operational challenges inherent reprogramming. organoids, offer an advanced platform complex neurodevelopmental processes require extensive resources technical expertise. which fetal macrophages located in placenta share common developmental origin uniquely exposed prenatal and, depending barrier maturation, exhibit variable makes them particularly useful exploring programming microglial development. concludes that no single comprehensively captures all aspects development, it offers guidance selecting most appropriate based specific research objectives experimental constraints.

Language: Английский

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The when, what, and where of naturally-acquired microchimerism

Seminars in Immunopathology, Journal Year: 2025, Volume and Issue: 47(1)

Published: March 11, 2025

Language: Английский

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The Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samples

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 21, 2023

In 2015, we launched the mesoSPIM initiative (www.mesospim.org), an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, demand imaging larger samples at higher speed and resolution has increased, requiring major improvements in capabilities microscopy. Here, introduce next-generation ("Benchtop") with significantly increased field view, improved resolution, throughput, affordable cost simpler assembly compared to original version. We developed a new method testing objectives, enabling us select detection objectives optimal large-sensor sCMOS cameras. The achieves high spatial (1.5 μm laterally, 3.3 axially) across entire magnification up 20x, supports sample sizes ranging from sub-mm several centimetres, while being compatible multiple clearing techniques. microscope serves broad range applications neuroscience, developmental biology, even physics.

Language: Английский

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