Gelatin methacryloyl granular scaffolds for localized mRNA delivery DOI Creative Commons
Bruna G. Carvalho,

Aya Nakayama,

Hiromi Miwa

et al.

Aggregate, Journal Year: 2023, Volume and Issue: 5(2)

Published: Nov. 20, 2023

Abstract Messenger RNA (mRNA) therapy is the intracellular delivery of mRNA to produce desired therapeutic proteins. Developing strategies for local still required where direct intra‐articular injections are inappropriate targeting a specific tissue. The efficiency depends on protecting nucleic acids against nuclease‐mediated degradation and safe site‐specific delivery. Herein, novel mRNA‐releasing matrices based RGD‐moiety‐rich gelatin methacryloyl (GelMA) microporous annealed particle (MAP) scaffolds reported. GelMA concentration in aerogel‐based microgels (µgels) produced through microfluidic process, MAP stiffnesses, microporosity crucial parameters cell adhesion, spreading, proliferation. After being loaded with complexes, composed 10% µgels display excellent viability increasing infiltration, proliferation, gene transfer. achieved by sustained release complexes from adhesion scaffolds. These findings highlight that hybrid systems can achieve efficient protein expression delivering making them promising biomaterials tissue engineering.

Language: Английский

Biology-driven material design for ischaemic stroke repair DOI
Nhi V. Phan,

Elle M. Rathbun,

Yunxin Ouyang

et al.

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 2(1), P. 44 - 63

Published: Oct. 27, 2023

Language: Английский

Citations

6
Piezotronic effect for in situ electrostimulation of neural stem cell therapy for nerve injury DOI
Wenhan Wang,

Keyi Li,

Bowei Xia

et al.

Nano Energy, Journal Year: 2023, Volume and Issue: 120, P. 109181 - 109181

Published: Dec. 9, 2023

Language: Английский

Citations

6
Lung metastasis-Harnessed in-Situ adherent porous organic nanosponge-mediated antigen capture for A self-cascaded detained dendritic cells and T cell infiltration DOI

Ting-Hsien Wu,

Yu‐Jen Lu,

Min‐Ren Chiang

et al.

Biomaterials, Journal Year: 2023, Volume and Issue: 305, P. 122443 - 122443

Published: Dec. 27, 2023

Language: Английский

Citations

6
Bio-inspired microvascular patches with MSC encapsulation for wound healing DOI
Rui Liu, Bin Kong, Jingjing Gan

et al.

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 476, P. 146608 - 146608

Published: Oct. 11, 2023

Language: Английский

Citations

5
Gelatin methacryloyl granular scaffolds for localized mRNA delivery DOI Creative Commons
Bruna G. Carvalho,

Aya Nakayama,

Hiromi Miwa

et al.

Aggregate, Journal Year: 2023, Volume and Issue: 5(2)

Published: Nov. 20, 2023

Abstract Messenger RNA (mRNA) therapy is the intracellular delivery of mRNA to produce desired therapeutic proteins. Developing strategies for local still required where direct intra‐articular injections are inappropriate targeting a specific tissue. The efficiency depends on protecting nucleic acids against nuclease‐mediated degradation and safe site‐specific delivery. Herein, novel mRNA‐releasing matrices based RGD‐moiety‐rich gelatin methacryloyl (GelMA) microporous annealed particle (MAP) scaffolds reported. GelMA concentration in aerogel‐based microgels (µgels) produced through microfluidic process, MAP stiffnesses, microporosity crucial parameters cell adhesion, spreading, proliferation. After being loaded with complexes, composed 10% µgels display excellent viability increasing infiltration, proliferation, gene transfer. achieved by sustained release complexes from adhesion scaffolds. These findings highlight that hybrid systems can achieve efficient protein expression delivering making them promising biomaterials tissue engineering.

Language: Английский

Citations

5