X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT DOI Creative Commons
Arne Maes, Onno Borgel,

Clara Braconnier

et al.

International Journal of Biomedical Imaging, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 15

Published: April 9, 2024

The kidney's microstructure, which comprises a highly convoluted tubular and vascular network, can only be partially revealed using classical 2D histology. Considering that the microstructure is closely related to its function often affected by pathologies, there need for powerful high-resolution 3D imaging techniques visualize microstructure. Here, we present how cryogenic contrast-enhanced microCT (cryo-CECT) allowed visualization of glomeruli, tubuli, vasculature. By comparing different contrast-enhancing staining agents freezing protocols, found preferred sample preparation protocol was combination with 1:2 hafnium(IV)-substituted Wells-Dawson polyoxometalate submersion in isopentane at -78°C. This optimized showed sensitive, allowing detect small pathology-induced microstructural changes mouse model mild trauma-related acute kidney injury after thorax trauma hemorrhagic shock. In summary, demonstrated cryo-CECT an effective histopathological tool allows enhance our understanding tissue their function.

Language: Английский

Beneath the Perforators: Unravelling Fascia Lata Intrinsic Vascular Architecture using microfocus X-ray computed tomography DOI Creative Commons
Julie Manon,

Eléonore Gillerot,

Frédéric Delandmeeter

et al.

Annals of Anatomy - Anatomischer Anzeiger, Journal Year: 2025, Volume and Issue: unknown, P. 152386 - 152386

Published: Feb. 1, 2025

Language: Английский

Citations

0
X-Ray-Based 3D Histo(patho)logy of Biological Tissues Using Contrast-Enhanced MicroCT DOI
Arne Maes, Tim Balcaen, Camille Pestiaux

et al.

Reference series in biomedical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 43

Published: Jan. 1, 2025

Language: Английский

Citations

0
Human cochlear microstructures at risk of electrode insertion trauma, elucidated in 3D with contrast-enhanced microCT DOI Creative Commons
Anastasiya Starovoyt, Grzegorz Pyka, Tristan Putzeys

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Feb. 7, 2023

Abstract Cochlear implant restores hearing loss through electrical stimulation of the nerve from within cochlea. Unfortunately, surgical implantation this neuroprosthesis often traumatizes delicate intracochlear structures, resulting in residual and compromising noisy environments appreciation music. To avoid cochlear trauma, insertion techniques devices have to be adjusted microanatomy. However, existing were unable achieve a representative visualization human cochlea: classical histology damages tissues lacks 3D perspective; standard microCT fails resolve soft tissues; previously used X-ray contrast-enhancing staining agents are destructive. In study, we overcame these limitations by performing contrast-enhanced imaging (CECT) with novel polyoxometalate agent Hf-WD POM. With POM-based CECT, achieved nondestructive, high-resolution, simultaneous, mineralized microstructures fresh-frozen cochleae. This enabled quantitative analysis true dimensions led anatomical discoveries, concerning surgically-relevant microstructures: round window membrane, Rosenthal’s canal secondary spiral lamina. Furthermore, demonstrated that CECT enables assessment structures as well their trauma.

Language: Английский

Citations

9
An optically-guided cochlear implant sheath for real-time monitoring of electrode insertion into the human cochlea DOI Creative Commons
Anastasiya Starovoyt,

Bryden C. Quirk,

Tristan Putzeys

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Nov. 10, 2022

Abstract In cochlear implant surgery, insertion of perimodiolar electrode arrays into the scala tympani can be complicated by trauma or even accidental translocation array within cochlea. patients with partial hearing loss, not only negatively affect performance, but also reduce residual function. These events have been related to suboptimal positioning respect critical walls (modiolar wall, osseous spiral lamina and basilar membrane). Currently, position in relation these cannot assessed during surgeon depends on tactile feedback, which is unreliable often comes too late. This study presents an image-guided device integrated, fiber-optic imaging probe that provides real-time feedback using optical coherence tomography human novel enables accurately detect identify ahead adjust trajectory, avoiding collision trauma. The functionality this prototype has demonstrated a series experiments, conducted experienced surgeons fresh-frozen cadaveric cochleae.

Language: Английский

Citations

14
3D Visualization of cyanobacterial biofilms using micro-computed tomography with contrast-enhancing staining agents DOI Creative Commons
Laurenz Schröer, Tim Balcaen, Karel Folens

et al.

Tomography of Materials and Structures, Journal Year: 2024, Volume and Issue: 4, P. 100024 - 100024

Published: Jan. 23, 2024

Currently, biofilms colonizing surfaces are mainly imaged in 2D by conventional techniques, such as optical or scanning electron microscopy. Confocal laser microscopy coherence tomography can visualize 3D, but they suffer from a limited penetration depth and cannot opaque materials. Micro-computed (µCT) overcome these issues, µCT easily distinguish biofilm structures water due to lack of contrast difference. Within this research, five contrast-enhancing staining agents (CESAs) were evaluated for their potential cyanobacterial biofilms, aiming 3D. Isotonic Lugol 1:2 hafnium(IV)-substituted Wells-Dawson polyoxometalate (Hf-WD POM) the most promising, allowed visualization revealed stained biofilms. Staining with isotonic could clearly bundles filaments within biofilm, while Hf-WD POM smooth biofilm. It is assumed that both CESAs have different affinity towards thus be used complementary. Monolacunary (Mono-WD showed moderate discrimination cationic iodinated CA4+ Hexabrix® (Guerbet) containing anionic ioxaglate did not allow distinctly These results indicate µCT, together POM, tool image extensive microbial mats Further research will determine whether suitable visualizing porous media.

Language: Английский

Citations

2
Collagen molecular organization preservation in human fascia lata and periosteum after tissue engineering DOI Creative Commons
Julia Vettese, Julie Manon, Antoine Chretien

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: April 3, 2024

Large bone defect regeneration remains a major challenge for orthopedic surgeons. Tissue engineering approaches are therefore emerging in order to overcome this limitation. However, these processes can alter some of essential native tissue properties such as intermolecular crosslinks collagen triple helices, which known their role structure and function. We assessed the persistence extracellular matrix (ECM) human fascia lata (HFL) periosteum (HP) after decellularization sterilization. Harvested from cadaveric donors (N = 3), samples each HFL HP were decellularized following five different chemical protocols with without detergents (D1-D4 D5, respectively). D1 D4 consisted combinations Triton, Sodium dodecyl sulfate Deoxyribonuclease, while D5 is routinely used institutional bank. Decellularized tissues further gamma-irradiated (minimum 25 kGy) study impact sterilization on ECM. Polarized light microscopy (PLM) was estimate thickness density fibers. hydration content hydroxyproline, enzymatic crosslinks, non-enzymatic (pentosidine) semi-quantified Raman spectroscopy. ELISA also analyze maintenance decorin (DCN), an important small leucine rich proteoglycan fibrillogenesis. Among protocols, detergent-free treatments tended disorganize samples, more thin fibers (+53.7%) less thick ones (−32.6%) recorded, well (−25.2%, p 0.19) significant decrease DCN ( 0.036). GAG significantly reduced both types all protocols. On other hand, sensitive detergent-based treatments, disrupted organization greater, though not loss (−37.4%, 0.137). Irradiation led (−29.4%, 0.037) than what observed process. Overall, results suggest that did matrix. addition gamma-irradiation deleterious structural integrity tissue.

Language: Английский

Citations

2
Exploring contrast-enhancing staining agents for studying adipose tissue through contrast-enhanced computed tomography DOI Creative Commons
Tim Balcaen, Andrea Beňová, Flip de Jong

et al.

Journal of Lipid Research, Journal Year: 2024, Volume and Issue: 65(7), P. 100572 - 100572

Published: May 30, 2024

Contrast-enhanced computed tomography offers a nondestructive approach to studying adipose tissue in 3D. Several contrast-enhancing staining agents (CESAs) have been explored, whereof osmium tetroxide (OsO

Language: Английский

Citations

2
Screening staining agents for contrast-enhanced microCT of vascular tissues: Assessing the effect on microstructural and mechanical properties DOI Creative Commons
Maïté Pétré, Tim Balcaen,

Pierre Schneidewind

et al.

Tomography of Materials and Structures, Journal Year: 2024, Volume and Issue: 6, P. 100038 - 100038

Published: June 29, 2024

Cardiovascular tissues possess a complex microstructure, which remodel and adapt due to ageing diseases. This evolving microstructure is intrinsically linked the tissue's mechanical properties. To better understand how changes in can impact behavior, 4D-contrast-enhanced microCT (4D-CECT) be used (i.e. situ loading combined with 3D microstructural visualization). Since absorption-based CECT requires use of contrast-enhancing staining agents (CESAs), we investigated six different CESAs for their suitability 4D-CECT imaging arterial tissue, considering ability provide good visualization segmentation while ensuring preservation For this purpose, penetration speed, contrast-enhancement, volume change, stiffness change porcine tissue stained CESA solutions were studied. Based on our results, selected 1:2 Hafnium-substituted Wells-Dawson Polyoxometalate as most suited tissue. Phosphotungstic acid (PTA) Lugol iodine Sorensen's buffer (Lugol), despite being reference state-of-the-art having excellent contrast-enhancement properties, only ones that significantly affected properties Additionally, these two solutions, shrinkage was observed, resulting reduction approximately − 12 % PTA 17 Lugol. Finally, it observed speed all exhibited ratio 60–40 from intimal side adventitial side, likely denser packing elastic lamellae towards adventitia. Overall, study offers valuable new insights selecting comparing various (4D-)CECT.

Language: Английский

Citations

2
A single Angiofil-latex injection for both radiological and anatomical assessment of arterial territories in the limbs DOI Creative Commons
Julie Manon,

Gaspary Fodjeu,

Robin Evrard

et al.

Annals of Anatomy - Anatomischer Anzeiger, Journal Year: 2024, Volume and Issue: 256, P. 152312 - 152312

Published: July 25, 2024

Postmortem evaluation of the human vascular system has a long history, with advancements ranging from dissections to modern imaging techniques like computed tomography (CT scan). This study designs novel combination Angiofil, liquid radiopaque polymer, and latex, flexible cast material, for cadaveric analysis.

Language: Английский

Citations

2
2D and 3D microstructural analysis of the iliotibial tract DOI
Julie Manon,

Laurie Gallant,

Paul Gérard

et al.

Journal of Anatomy, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Abstract The fascial system has gained recognition for its integral role in connecting skin, superficial and deep fasciae, underlying muscles. However, consensus on microstructure depending topography remains elusive as well implications clinical practices, such reconstructive surgery physiotherapy techniques. This study focuses the iliotibial tract (ITT) implicated band syndrome. goal is to describe microstructural characteristics using classical 2D histology cryogenic contrast‐enhanced microcomputed tomography (cryo‐CECT) total thickness, number of layers, layer fibre orientation tortuosity, according specific topography. thickness ITT varied across topographic regions, with superior part being average thicker but non‐significantly different from other regions. inferior showed heterogeneity, anterior region (AI) thinnest posterior one (PI) thickest. exhibited 1–3 no significant differences among Most commonly, it consisted two except antero‐superior (AS) antero‐middle (AM) which sometimes had only layer. regions frequently 2 or 3 PI having highest mean (2.7 layers). intermediate was thickest one, varying AI (0.368 mm ± 0.114) (0.640 0.305). regional variability, AS thinnest. appeared thinner than one. Fibre analysis indicated that mainly oblique longitudinal fibres, orientated downward forward, while layers transversal fibres. Cryo‐CECT 3D observations confirmed these findings, revealing distinct orientations layers. tortuosity based orientation. Transversal fibres (>65°) were significantly less tortuous (<25°) (25°–65°), aligning plot observations. quantitative highlights various ITT, offering insights into variations. accuracy increased due novel technology cryo‐CECT emerges a valuable tool precise assessment tortuosity. These findings contribute deeper understanding structure, useful physiotherapy, future research endeavours.

Language: Английский

Citations

2