The role of lysophosphatidic acid and its receptors in corneal nerve regeneration DOI Creative Commons
Maryam Kheyrollah, Nicola Brandt, Anja U. Bräuer

et al.

The Ocular Surface, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

The integrity of corneal nerves is critical for ocular surface health, and damages can lead to Neurotrophic Keratopathy (NK). Despite the regenerative abilities peripheral nerve system (PNS), regeneration often incomplete, underlying mechanisms are poorly understood. This study aims identify potential factors that enhance NK treatment, with a focus on Lysophosphatidic acid (LPA). Thus, effect LPA its pathways in investigated detail using vitro mouse sensory neurons. To elucidate impact as well reveal responsible receptor, several functional assays siRNA-based knock-down experiments were conducted. Additionally, possible changes mRNA levels. LPA-treated neurons significantly reduced fiber growth. However, LPAR2 knockdown (Lpar2-KD) following treatment showed significant increase length. demonstrated enhanced levels Lpar2 mRNA. On other hand, indicators such Ngf, Gap-43, Cdc42, along downstream signaling components like Pi3k Ras, elevated Lpar2-KD In conclusion, this elucidates inhibitory effects outgrowth Furthermore, was identified receptor effect. might be promising therapeutic approach neuronal patients NK.

Language: Английский

Suppression of autophagy induces senescence in the heart DOI Open Access
Peiyong Zhai, Eun‐Ah Sung,

Yuka Shiheido‐Watanabe

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 30, 2024

Aging is a critical risk factor for heart disease, including ischemic disease and failure. Cellular senescence, characterized by DNA damage, resistance to apoptosis the senescence-associated secretory phenotype (SASP), occurs in many cell types, cardiomyocytes. Senescence precipitates aging process surrounding cells organ through paracrine mechanisms. Generalized autophagy, which degrades cytosolic materials non-selective manner, decreased during heart. This decrease causes deterioration of cellular quality control mechanisms, facilitates negatively affects lifespan animals, mice. Although suppression generalized autophagy could promote it remains unclear whether directly stimulates senescence cardiomyocytes, which, turn, promotes myocardial dysfunction We addressed this question using mouse models with loss function. Suppression general cardiac-specific

Language: Английский

Citations

0
Amelioration of retinal injury and improvement in associated memory by hUCB-derived cells is dose-dependent DOI

Priya Mehra,

Manjari Rain, Jagtar Singh

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Abstract Background- The visual information transfers from retina to brain amplify the neuronal signals resulting in perception. Damage retinal layer causes field defects such as cognition decline and memory loss. Although, various research investigations have attempted reverse damage using therapeutic interventions, however, these not been successfully translated. In this study we aim evaluate efficacy of lineage negative stem cells derived hUCB reversing injury by subretinal transplantation mice model laser injury. Methods- Retinal was introduced C57BL/6J male (24-28g) photocoagulation around optic disc with defined parameters that disrupted RPE retina. 2 spots (2L) 8 (8L) were created each eye mice. Fundus Fluorescein Angiography used confirm establishment varying degree Around 50,000 transplanted after 24 hours After 1 month, neurobehavioral assessments carried out estimate visual-spatial Morris Water Maze (MWM) Passive Avoidance. Subsequently, molecular assays including RT-PCR immunohistochemistry also undertaken. Results- angiography confirmed presence vein leakage comparison healthy control. To identify cognitive deficits, assessments, based on water maze passive avoidance, performed. tests show learning improvement cell group group. Further, gene expression neurotrophic factors, proliferative apoptotic factors showed upregulated activity possible neuroprotective role rescue Conclusion-The Lin –ve rescued reversed shows that, injury, number lin should be increased.

Language: Английский

Citations

0
WITHDRAWN: Amelioration of retinal injury and improvement in associated memory by hUCB-derived cells is dose-dependent DOI Creative Commons

Priya Mehra,

Manjari Rain, Jagtar Singh

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Abstract The full text of this preprint has been withdrawn, as it was submitted in error. Therefore, the authors do not wish work to be cited a reference. Questions should directed corresponding author.

Language: Английский

Citations

0
Molecular physiology unlocks the mystery that relates cognitive impairment with the retina in schizophrenia and autism spectrum disorders: a perspective review DOI Creative Commons
Sarah Al-Mazidi

Frontiers in Psychiatry, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 11, 2024

Schizophrenia and Autism spectrum disorders (SSD ASD) are neurodevelopmental involving cognitive impairment. Timely diagnosis is important for early intervention; currently, no tools available to help with diagnosis. Molecular biomarkers of impairment have been extensively studied, but clinical correlation crucial in screening SSD ASD. There has growing interest examining the retina scan neurological since only part central nervous system that can be directly imaged non-invasively a timely manner. This review discusses their It also possible involvement molecular biomarkers, specifically Disintegrin metalloproteinase domain-containing protein 10 (ADAM10) ciliary neurotrophic factor (CNTF) pathophysiology A protocol diagnosing its severity ASD suggested. mentions insights into potential use enhance performance areas where more research needed solve mystery relationship between psychiatric disorders.

Language: Английский

Citations

0
The role of lysophosphatidic acid and its receptors in corneal nerve regeneration DOI Creative Commons
Maryam Kheyrollah, Nicola Brandt, Anja U. Bräuer

et al.

The Ocular Surface, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

The integrity of corneal nerves is critical for ocular surface health, and damages can lead to Neurotrophic Keratopathy (NK). Despite the regenerative abilities peripheral nerve system (PNS), regeneration often incomplete, underlying mechanisms are poorly understood. This study aims identify potential factors that enhance NK treatment, with a focus on Lysophosphatidic acid (LPA). Thus, effect LPA its pathways in investigated detail using vitro mouse sensory neurons. To elucidate impact as well reveal responsible receptor, several functional assays siRNA-based knock-down experiments were conducted. Additionally, possible changes mRNA levels. LPA-treated neurons significantly reduced fiber growth. However, LPAR2 knockdown (Lpar2-KD) following treatment showed significant increase length. demonstrated enhanced levels Lpar2 mRNA. On other hand, indicators such Ngf, Gap-43, Cdc42, along downstream signaling components like Pi3k Ras, elevated Lpar2-KD In conclusion, this elucidates inhibitory effects outgrowth Furthermore, was identified receptor effect. might be promising therapeutic approach neuronal patients NK.

Language: Английский

Citations

0