Algae obscura: The potential of rare species as model systems DOI Creative Commons
Julia Van Etten, L. Felipe Benites, Timothy G. Stephens

et al.

Journal of Phycology, Journal Year: 2023, Volume and Issue: 59(2), P. 293 - 300

Published: Feb. 11, 2023

Model organism research has provided invaluable knowledge about foundational biological principles. However, most of these studies have focused on species that are in high abundance, easy to cultivate the lab, and represent only a small fraction extant biodiversity. Here, we present three examples rare algae with unusual features refer as "algae obscura." The Cyanidiophyceae (Rhodophyta), Glaucophyta, Paulinella (rhizarian) lineages all transitioned out obscurity become models for fundamental evolutionary research. Insights been gained into prevalence importance eukaryotic horizontal gene transfer, early Earth microbial community dynamics, primary plastid endosymbiosis, origin Archaeplastida. By reviewing come from exploration organisms, demonstrate underappreciated potential help us formulate, refine, substantiate core hypotheses such organisms should be considered when establishing future model systems.

Language: Английский

Dual–Acceptor Engineering in Pyrene‐Based Covalent Organic Frameworks for Boosting Photocatalytic Hydrogen Evolution DOI

Nengyi Liu,

Shuailei Xie, Yuxing Huang

et al.

Advanced Energy Materials, Journal Year: 2024, Volume and Issue: 14(40)

Published: July 18, 2024

Abstract The integration of electron donor (D) and acceptor (A) units into covalent organic frameworks (COFs) has received increasing interest due to its potential for efficient photocatalytic hydrogen (H 2 ) evolution from water. Nevertheless, the advancement D–A COFs is still constrained by limited investigations on engineering, which enables highly effective charge transfer pathways in deliver photoexcited electrons a preferential orientation enhance performance. Herein, two systems with D–A–A configurations based molecular engineering strategy are proposed construct three distinct COFs. Specifically, TAPPy‐DBTDP‐COF merging one pyrene‐based benzothiadiazole acceptors realized an average H rate 12.7 mmol h −1 g under visible light, among highest ever reported typical D–A‐type COF systems. combination experimental theoretical analysis signifies crucial role dual‐acceptor arrangement promoting exciton dissociation carrier migration. These findings underscore significant structural design, conducive separation holes resulting superior activities.

Language: Английский

Citations

25
The structure of PSI-LHCI from Cyanidium caldarium provides evolutionary insights into conservation and diversity of red-lineage LHCs DOI Creative Commons
Koji Kato, Tasuku Hamaguchi, Minoru Kumazawa

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(11)

Published: March 5, 2024

Light-harvesting complexes (LHCs) are diversified among photosynthetic organisms, and the structure of photosystem I-LHC (PSI-LHCI) supercomplex has been shown to be variable depending on species organisms. However, structural evolutionary correlations red-lineage LHCs unknown. Here, we determined a 1.92-Å resolution cryoelectron microscopic PSI-LHCI isolated from red alga Cyanidium caldarium RK-1 (NIES-2137), which is an important taxon in Cyanidiophyceae. We subsequently investigated PSI-LHCIs different organisms through comparisons phylogenetic analysis. The obtained shows five LHCI subunits surrounding PSI-monomer core. LHCIs composed two Lhcr1s, Lhcr2s, one Lhcr3. Phylogenetic analysis bound PSI algae showed clear orthology between C. Cyanidioschyzon merolae , whereas no orthologous relationships were found Lhcr1–3 other structures. These findings provide insights into conservation diversity associated with PSI.

Language: Английский

Citations

20
The Polycomb repressive complex 2 deposits H3K27me3 and represses transposable elements in a broad range of eukaryotes DOI Creative Commons
Tetsuya Hisanaga, Facundo Romani, Shuangyang Wu

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(20), P. 4367 - 4380.e9

Published: Sept. 21, 2023

The mobility of transposable elements (TEs) contributes to evolution genomes. Their uncontrolled activity causes genomic instability; therefore, expression TEs is silenced by host are marked with DNA and H3K9 methylation, which associated silencing in flowering plants, animals, fungi. However, distantly related groups eukaryotes, H3K27me3 deposited the Polycomb repressive complex 2 (PRC2), an epigenetic mark gene plants animals. direct PRC2 has so far only been shown one species ciliates. To test if silences a broader range we generated mutants reduced analyzed role extant along lineage Archaeplastida diatom P. tricornutum. In this red alga C. merolae, greater proportion than genes were repressed PRC2, whereas bryophytes. contained potential cis-elements recognized transcription factors neighbor as transcriptional units PRC2. Thus, observed not but also diatoms ciliates, suggesting that silence last common ancestor eukaryotes. We hypothesize during Archaeplastida, TE fragments selected shape regulation, controlling networks regulated

Language: Английский

Citations

30
Revised classification of the Cyanidiophyceae based on plastid genome data with descriptions of the Cavernulicolales ord. nov. and Galdieriales ord. nov. (Rhodophyta) DOI Open Access

Seung In Park,

Chung Hyun Cho, Claudia Ciniglia

et al.

Journal of Phycology, Journal Year: 2023, Volume and Issue: 59(3), P. 444 - 466

Published: Feb. 16, 2023

Abstract The Cyanidiophyceae, an extremophilic red algal class, is distributed worldwide in extreme environments. Species grow either acidic hot environments or dim light conditions (e.g., “cave Cyanidium ”). taxonomy and classification systems are currently based on morphological, eco‐physiological, molecular phylogenetic characters; however, previous results showed hidden diversity of the Cyanidiophyceae suggested a revision system. To clarify relationships within this we employ phylogenomic approach 15 plastomes (10 new) mitogenomes (seven new). Our phylogenies show consistent among four lineages ( Galdieria , ”, Cyanidioschyzon lineages). Each lineage distinguished by organellar genome characteristics. ” distinct clade that diverged after but larger monophyletic included lineages. Because mesophilic differs substantially from other three thermoacidophilic lineages, describe it as new order (Cavernulicolales). Based evidence, reclassified into orders: Cyanidiales, Cyanidioschyzonales, Cavernulicolales ord. nov., Galdieriales nov. genetic distance these orders comparable to, greater than, distances found between subclasses. Three genera Cavernulicola Gronococcus, Sciadococcus ), five species javensis phlegrea, yellowstonensis Gronococcus sybilensis, taiwanensis nomenclatural combination chilensis ) proposed.

Language: Английский

Citations

21
Red macroalgae in the genomic era DOI Creative Commons
Michael Borg, Stacy A. Krueger‐Hadfield, Christophe Destombe

et al.

New Phytologist, Journal Year: 2023, Volume and Issue: 240(2), P. 471 - 488

Published: Aug. 30, 2023

Summary Rhodophyta (or red algae) are a diverse and species‐rich group that forms one of three major lineages in the Archaeplastida, eukaryotic supergroup whose plastids arose from single primary endosymbiosis. Red algae united by several features, such as relatively small intron‐poor genomes lack cytoskeletal structures associated with motility like flagella centrioles, well highly efficient photosynthetic capacity. Multicellular macroalgae) earliest diverging to have evolved complex multicellularity, yet despite their ecological, evolutionary, commercial importance, they remained largely understudied organisms. Considering increasing availability algal genome sequences, we present broad overview fundamental aspects macroalgal biology posit on how this is expected accelerate research many domains coming years.

Language: Английский

Citations

21
Hot springs viruses at Yellowstone National Park have ancient origins and are adapted to thermophilic hosts DOI Creative Commons
L. Felipe Benites, Timothy G. Stephens, Julia Van Etten

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: April 9, 2024

Abstract Geothermal springs house unicellular red algae in the class Cyanidiophyceae that dominate microbial biomass at these sites. Little is known about host-virus interactions environments. We analyzed virus community associated with algal mats three neighboring habitats (creek, endolithic, soil) Lemonade Creek, Yellowstone National Park (YNP), USA. find despite proximity, each habitat houses a unique collection of viruses, giant Megaviricetes, dominant all three. The early branching phylogenetic position genes encoded on metagenome assembled genomes (vMAGs) suggests YNP lineages are ancient origin and not due to multiple invasions from mesophilic habitats. existence genomic footprints adaptation thermophily vMAGs consistent this idea. geothermal sites originated ca. 1.5 Bya therefore relevant understanding biotic Earth.

Language: Английский

Citations

7
Origin of minicircular mitochondrial genomes in red algae DOI Creative Commons
Yongsung Lee, Chung Hyun Cho, Chanyoung Noh

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: June 8, 2023

Abstract Eukaryotic organelle genomes are generally of conserved size and gene content within phylogenetic groups. However, significant variation in genome structure may occur. Here, we report that the Stylonematophyceae red algae contain multipartite circular mitochondrial (i.e., minicircles) which encode one or two genes bounded by a specific cassette constant region. These minicircles visualized using fluorescence microscope scanning electron microscope, proving circularity. Mitochondrial sets reduced these highly divergent mitogenomes. Newly generated chromosome-level nuclear assembly Rhodosorus marinus reveals most ribosomal subunit transferred to genome. Hetero-concatemers resulted from recombination between unique inventory is responsible for stability explain how transition typical occurs. Our results offer inspiration on minicircular formation highlight an extreme case reduction.

Language: Английский

Citations

14
Mechanisms of adaptation and evolution in Toxoplasma gondii DOI
Sergio O. Ángel, Laura Vanagas, Andrés M. Alonso

et al.

Molecular and Biochemical Parasitology, Journal Year: 2024, Volume and Issue: 258, P. 111615 - 111615

Published: Feb. 13, 2024

Language: Английский

Citations

5
Identification of plants’ functional counterpart of the metazoan mediator of DNA Damage checkpoint 1 DOI
Zdravko J. Lorković, Michael Klingenbrunner, Chung Hyun Cho

et al.

EMBO Reports, Journal Year: 2024, Volume and Issue: 25(4), P. 1936 - 1961

Published: March 4, 2024

Language: Английский

Citations

5
Diverse fates of ancient horizontal gene transfers in extremophilic red algae DOI Creative Commons
Julia Van Etten, Timothy G. Stephens, Erin Chille

et al.

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(5)

Published: May 1, 2024

Abstract Horizontal genetic transfer (HGT) is a common phenomenon in eukaryotic genomes. However, the mechanisms by which HGT‐derived genes persist and integrate into other pathways remain unclear. This topic of significant interest because, over time, stressors that initially favoured fixation HGT may diminish or disappear. Despite this, foreign continue to exist if they become part broader stress response pathways. The conventional model suggests acquisition equates adaptation. this evolve more complex interactions between gene products, concept we refer as ‘Integrated Model’ (IHM). To explore further, studied specialized encode heavy metal detoxification functions. recruitment these could provide clear examples IHM. In our study, exposed two anciently diverged species polyextremophilic red algae from Galdieria genus arsenic mercury laboratory cultures. We then analysed transcriptome data using differential coexpression analysis. Our findings revealed follows ‘one gene‐one function’ model, resulting an indivisible response. contrast, ars H arsenite pathway demonstrated pattern duplication, divergence potential neofunctionalization, consistent with research sheds light on fate integration ancient HGTs, providing novel perspective ecology extremophiles.

Language: Английский

Citations

5