Selenomonas sputigena acts as a pathobiont mediating spatial structure and biofilm virulence in early childhood caries

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: May 22, 2023

Abstract Streptococcus mutans has been implicated as the primary pathogen in childhood caries (tooth decay). While role of polymicrobial communities is appreciated, it remains unclear whether other microorganisms are active contributors or interact with pathogens. Here, we integrate multi-omics supragingival biofilm (dental plaque) from 416 preschool-age children (208 males and 208 females) a discovery-validation pipeline to identify disease-relevant inter-species interactions. Sixteen taxa associate metagenomics-metatranscriptomics analyses. Using multiscale/computational imaging virulence assays, examine formation dynamics, spatial arrangement, metabolic activity Selenomonas sputigena, Prevotella salivae Leptotrichia wadei , either individually S. . We show that sputigena flagellated anaerobe previously unknown biofilm, becomes trapped streptococcal exoglucans, loses motility but actively proliferates build honeycomb-like multicellular-superstructure encapsulating enhancing acidogenesis. Rodent model experiments reveal an unrecognized ability colonize tooth surfaces. incapable causing on its own, when co-infected mutans, causes extensive enamel lesions exacerbates disease severity vivo. In summary, discover pathobiont cooperating known unique structure heighten prevalent human disease.

Language: Английский

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Caries Etiology and Preventive Measures

European Journal of Dentistry, Journal Year: 2024, Volume and Issue: 18(03), P. 766 - 776

Published: March 31, 2024

Abstract Caries is a widespread disease in both children and adults. caused by the conversion of fermentable carbohydrates plaque bacteria into acids on tooth surface. Thus, it important to focus sugar reduction control. For efficient removal/control, state-of-the-art toothpastes contain various active ingredients such as antimicrobial agents (e.g., chlorhexidine, stannous salts, zinc salts), abrasives calcium carbonate, phosphates, hydrated silica), surfactants sodium lauryl sulfate methyl cocoyl taurate), natural compounds polyphenols xylitol). Agents with pH-buffering calcium-releasing properties carbonate phosphates) biomimetic actives hydroxyapatite) reverse effects acids. Additionally, modern toothbrushes (i.e., electric toothbrushes) well dental floss interdental brushes significantly help remove from surfaces including interproximal surfaces. In conclusion, concepts caries prevention should not only remineralization alone but also control all key factors involved development.

Language: Английский

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Saliva and tongue microbiota in burning mouth syndrome: An exploratory study of potential roles

Oral Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: April 3, 2024

Abstract Objectives Burning mouth syndrome (BMS) is a chronic orofacial pain disorder with unclear etiology, in which the tongue most commonly affected. This study aims to provide implication of possible relationship between oral microbiota and pathogenesis BMS. Materials Methods Saliva swabs 15 primary BMS patients 10 healthy controls were collected assessed by 16S rRNA gene amplicon sequencing. The compositions compared bioinformatic analysis was conducted. Results Differences revealed both saliva samples. In saliva, Streptococcus , Rothia Neisseria predominant genus at taxonomic level patients. samples, Prevotella dominant LEfSe linear discriminant score showed that Actinobacteria phylum Selenomonas enriched dorsum Conclusions for first‐time reported profiles distinguished from controls, indicating necessity further research on microbes

Language: Английский

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Lysine aggregates‐based nanostructured antimicrobial peptides for cariogenic biofilm microenvironment‐activated caries treatment

Aggregate, Journal Year: 2024, Volume and Issue: 5(5)

Published: April 30, 2024

Abstract Dental caries is one of the most prevalent and costly biofilm‐induced oral diseases that causes deterioration mineralized tooth tissue. Traditional antimicrobial agents like antibiotics peptides (AMPs) struggle to effectively eradicate bacteria in biofilms without eliciting resistance. Herein, we demonstrate construction FeOOH@Fe‐Lysine@Au nanostructured AMPs (nAMPs) distinguished by their AMP‐like antibacterial activity self‐producing reactive oxygen species (ROS) capacity for treatment. On hand, nAMPs can catalyze glucose oxidation generate ROS within cariogenic biofilm microenvironment, resulting disintegration extracellular polymeric substance matrix exposure bacteria. other attach bacterial surfaces via electrostatic attractions, proceeding damage membranes, disrupt metabolic pathways, inhibit protein synthesis through aggregated lysine generated ROS. Based on this mechanism, Streptococcus mutans its associated biofilm, significantly impeding progression dental caries. Given straightforward cost‐efficient preparation compared require specific sequences, minimal adverse impacts gingival/palatal tissues, major organs, oral/gut microbiomes, our research may promote development novel therapeutic health maintenance.

Language: Английский

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Interspecies metabolite transfer fuels the methionine metabolism of Fusobacterium nucleatum to stimulate volatile methyl mercaptan production

mSystems, Journal Year: 2024, Volume and Issue: 9(2)

Published: Jan. 30, 2024

Halitosis can have a significant impact on the social life of affected individuals. Among oral odor compounds, CH 3 SH has low olfactory threshold and halitosis is result its production. Recently, there been growing interest in collective properties polymicrobial communities, regarded as important for development diseases, which are shaped by physical metabolic interactions among community participants. However, it yet to be investigated whether interspecies an production volatile leading halitosis. The present findings provide mechanistic insights indicating that ornithine, metabolite excreted Streptococcus gordonii , promotes polyamine synthesis Fusobacterium nucleatum resulting compensatory increase demand methionine, results elevated methionine pathway activity Elucidation mechanisms related expected lead new strategies managing

Language: Английский

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Methodological Considerations in Longitudinal Analyses of Microbiome Data: A Comprehensive Review

Genes, Journal Year: 2023, Volume and Issue: 15(1), P. 51 - 51

Published: Dec. 28, 2023

Biological processes underlying health and disease are inherently dynamic best understood when characterized in a time-informed manner. In this comprehensive review, we discuss challenges inherent time-series microbiome data analyses compare available approaches methods to overcome them. Appropriate handling of longitudinal can shed light on important roles, functions, patterns, potential interactions between large numbers microbial taxa or genes the context health, disease, interventions. We present review comparison existing analysis methods, for both preprocessing downstream analyses, including differential analysis, clustering, network inference, trait classification. posit that careful selection appropriate utilization computational tools help advance our understanding host–microbiome relationships underlie health-maintaining homeostases, progressions disease-promoting dysbioses, as well phases physiologic development like those encountered childhood.

Language: Английский

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The short‐term impact of comprehensive caries treatment on the supragingival microbiome of severe early childhood caries

International Journal of Paediatric Dentistry, Journal Year: 2024, Volume and Issue: 34(5), P. 505 - 515

Published: Jan. 3, 2024

Children affected by severe early childhood caries (S-ECC) usually need comprehensive treatment due to the extensive of caries. How oral microbiome changes after therapy within short-term warrant further study.

Language: Английский

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Biomimetic Janus Particles Induced In Situ Interfacial Remineralization for Dentin Hypersensitivity

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

Abstract Dentin hypersensitivity (DH), caused by the exposure of dentin tubules, is a common complaint dental patients. Although occlusion exposed tubules primary treatment approach, complex oral environment, and multiple simultaneous requirements often hinder its implementation. In this study, strawberry‐shaped hemispheric Janus particles (JPs) are synthesized, their use in DH evaluated vitro an animal model. The side JPs modified with polymers quaternary ammonium salts (QASs) to form superhydrophobic coating antibiofilm properties, while flat catechol groups able strong bonds dentin. Even after 1 h ultrasonication or 1000 rounds thermal cycling, completely occluded JPs. Moreover, biofilm formation not observed, area living bacteria less than 1% compared blank control sodium fluoride (NaF)‐treated groups. rat model, fixed specimens at day 3, much earlier obtained commonly used NaF. These results demonstrate that can provide novel approach DH.

Language: Английский

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Molecular Diagnosis and Therapy of Dental Caries by Oral Microbiome‐Selective Aggregation‐Induced Photosensitivity

Aggregate, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

ABSTRACT Dental caries represents one of the most widespread oral bacterial infections, affecting billions individuals worldwide and presenting significant public health challenges. Effective diagnosis treatment are hindered by limitations traditional clinical methodologies, which often involve laborious examinations invasive procedures. In this study, we employ an aggregation‐induced emission probe, MeOTpy, for instant molecular detection photodynamic dental caries. MeOTpy interacts specifically with cariogenic bacteria, exhibiting bright fluorescence upon binding to bacteria generating reactive oxygen species under white light irradiation. This photosensitivity enables rapid assessment carious disease through fluorescent in plaque samples, facilitating easy identification lesion sites decayed teeth treated MeOTpy. Furthermore, inhibition biofilms is achieved culturing ex vivo isolated from children severe early childhood also effectively prevents while preserving microbial diversity a infection model on rat pups. study presents integrated strategy precise at point care.

Language: Английский

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Screening for Selenomonas noxia in a Pediatric and Adolescent Patient Population Reveals Differential Oral Prevalence across Age Groups

International Journal of Environmental Research and Public Health, Journal Year: 2024, Volume and Issue: 21(4), P. 391 - 391

Published: March 23, 2024

Selenomonas noxia, a gram-negative anaerobe usually present in periodontitis, may be linked to overweight and obese adults. Recent advancements include valid qPCR screening, enabling an effective prevalence study among pediatric patients aged 7 17 years. The aim of this was complete retrospective screening saliva samples from existing biorepository using validated protocol. sample (n = 87) comprised nearly equal numbers males females, mostly minority (67%), with average age 13.2 Screening for noxia revealed 34.4% 30/87) positive samples, evenly distributed between females (p 0.5478). However, age-dependent association observed higher percentages ages (13.3% 10 years; 34.6% 11 13 54.8% 14–17 years), which statistically significant 0.0001). Although these findings no noteworthy distinctions or minorities non-minorities, the notable contrast younger (7 years) older (11 participants, possibly influenced by factors such as hormones behavioral traits, will require further investigation patient population.

Language: Английский

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