Environmental Microbiome, Journal Year: 2025, Volume and Issue: 20(1)
Published: April 24, 2025
Language: Английский
Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)
Published: June 13, 2023
Abstract Around 50% of humankind relies on groundwater as a source drinking water. Here we investigate the age, geochemistry, and microbiology 138 samples from 95 monitoring wells (<250 m depth) located in 14 aquifers Canada. The geochemistry show consistent trends suggesting large-scale aerobic anaerobic hydrogen, methane, nitrogen, sulfur cycling carried out by diverse microbial communities. Older groundwaters, especially with organic carbon-rich strata, contain average more cells (up to 1.4 × 10 7 mL −1 ) than younger challenging current estimates subsurface cell abundances. We observe substantial concentrations dissolved oxygen (0.52 ± 0.12 mg L [mean SE]; n = 57) older groundwaters that seem support metabolisms ecosystems at an unprecedented scale. Metagenomics, isotope analyses mixing models indicate dark is produced situ via dismutation. ancient sustain productive communities highlight overlooked present past Earth.
Language: Английский
Citations
61
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 28, 2025
Abstract Despite advances in dispersal mechanisms and risk assessment of antibiotic resistance genes (ARGs), how plants influence ARG contamination agricultural soils remains underexplored. Here, the impacts plant species diversity on ARGs mobile genetic elements (MGEs) three are comprehensively investigated a pot experiment. The results indicate that increased reduces MGEs abundance by 19.2%–51.2%, whereas exhibit inconsistent soil‐dependent effects. Potential bacterial hosts harboring abundant have greater relative than nonhosts, both their richness cumulative reduced diversity. Notably, inhibited present other hosts. enriched compounds root exudates due to play more important role metabolic network contribute rebalancing potential nonhosts. An independent test using pure organics reveals higher resource diversity, resulting from mobility high‐risk ARGs. This study highlights resource‐mediated mitigation risks posed indicates ensuring is promising strategy for controlling agroecosystems.
Language: Английский
Citations
2
Biogeochemistry, Journal Year: 2025, Volume and Issue: 168(1)
Published: Feb. 6, 2025
Nitrate pollution frequently impacts groundwater quality, particularly in agricultural regions across the world, but identifying sources of nitrate (NO3−) remains challenging. The extensive use nitrogen-containing fertilizers, surpassing crop requirements, and livestock management practices associated with spreading manure can lead to accumulation transport NO3− into groundwater, potentially affecting drinking water sources. We investigated occurrence distribution Southern Alberta, Canada, a region characterized by intensive cultivation industry. Over 3500 samples from provincial-scale quality database, collated multiple projects sources, involving domestic wells, monitoring springs, coupled newly obtained wells provided comprehensive geochemical insights quality. While stable isotope compositions (δ15N δ18O) were exclusively available for data instrumental constraining transformation processes within aquifers study region. Among all samples, 49% (n = 1746) concentrations below detection limits. Ten percent 369) including limits, exceed Canadian maximum acceptable concentration 10 mg/L as nitrogen (NO3−–N). Elevated (> NO3−–N) mainly detected at shallow depths (< 30 m) predominantly surficial sediments less bedrock aquifers. Statistical correlations between aqueous parameters showed positive associations NO3−–N both potassium (K+) chloride (Cl−), indicating influence synthetic fertilizers on In addition, analyses revealed three mineralization soil organic followed nitrification soils, ammonium or urea-based manure. However, was identified dominant source exceeding agriculturally dominated areas. Additionally, this multifaceted approach helped identify denitrification some process that plays key role reducing under favorable redox conditions methodological used be applied other worldwide removal contaminated aquifers, there are well networks place monitor
Language: Английский
Citations
2
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: June 21, 2024
Lacustrine methane emissions are strongly mitigated by aerobic methane-oxidizing bacteria (MOB) that typically most active at the oxic-anoxic interface. Although oxygen is required MOB for first step of oxidation, their occurrence in anoxic lake waters has raised possibility they capable oxidizing further anaerobically. Here, we investigate activity and growth Lake Zug, a permanently stratified freshwater lake. The rates anaerobic oxidation hypolimnion reached up to 0.2 µM d
Language: Английский
Citations
13
Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(28), P. 12532 - 12541
Published: June 28, 2024
While partial nitrification (PN) has the potential to reduce energy for aeration, it proven be unstable when treating low-strength wastewater. This study introduces an innovative combined strategy incorporating a low rate of oxygen supply, pH control, and sulfide addition selectively inhibit nitrite-oxidizing bacteria (NOB). led stable PN in laboratory-scale membrane aerated biofilm reactor (MABR). Over period 260 days, nitrite accumulation ratio exceeded 60% synthetic sewage containing 50 mg NH
Language: Английский
Citations
12
The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)
Published: Jan. 1, 2024
The terrestrial subsurface hosts microbial communities that, collectively, are predicted to comprise as many cells global surface soils. Although initially thought be associated with deposited organic matter, deep supported by chemolithoautotrophic primary production, hydrogen serving an important source of electrons. Despite recent progress, relatively little is known about the compared more commonly studied environments. Understanding composition and factors that influence them importance because human-associated activities including long-term storage used nuclear fuel, carbon capture, for use energy vector. In addition identifying microorganisms, research focuses on roles microorganisms in communities, well elucidating myriad interactions-syntrophic, episymbiotic, viral-that occur among community members. years, entirely new groups (i.e. candidate phyla radiation bacteria Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoloarchaeota, Nanoarchaeota archaea) have been discovered environments, suggesting much remains unknown this biosphere. This review explores historical context ecology highlights discoveries shape current ecological understanding poorly explored habitat. Additionally, we highlight need multifaceted experimental approaches observe phenomena such cryptic cycles, complex interactions, episymbiosis, which may not apparent when using single isolation, but nonetheless critical advancing our
Language: Английский
Citations
11
Technology in Society, Journal Year: 2025, Volume and Issue: unknown, P. 102821 - 102821
Published: Jan. 1, 2025
Language: Английский
Citations
1
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Aug. 29, 2024
The geosphere and the microbial biosphere have co-evolved for ~3.8 Ga, with many lines of evidence suggesting a hydrothermal habitat life's origin. However, extent that contemporary thermophiles their habitats reflect those likely existed on early Earth remains unknown. To address this knowledge gap, 64 geochemical analytes were measured 1022 metagenome-assembled-genomes (MAGs) generated from 34 chemosynthetic high-temperature springs in Yellowstone National Park analysed alongside 444 MAGs 35 published metagenomes. We used these data to evaluate co-variation MAG taxonomy, metabolism, phylogeny as function hot spring geochemistry. found cohorts functions are discretely distributed across pH gradients different provinces. Acidic or circumneutral/alkaline harbor branched later enriched sulfur- arsenic-based O
Language: Английский
Citations
6
Microbiome, Journal Year: 2023, Volume and Issue: 11(1)
Published: Sept. 11, 2023
Gypsum Hill Spring, located in Nunavut the Canadian High Arctic, is a rare example of cold saline spring arising through thick permafrost. It perennially discharges (~ 7 °C), hypersaline (7-8% salinity), anoxic 0.04 ppm O2), and highly reducing - 430 mV) brines rich sulfate (2.2 g.L-1) sulfide (9.5 ppm), making an analog to putative sulfate-rich briny habitats on extraterrestrial bodies such as Mars.Genome-resolved metagenomics metatranscriptomics were utilized describe active microbial community containing novel metagenome-assembled genomes dominated by sulfur-cycling Desulfobacterota Gammaproteobacteria. Sulfate reduction was hydrogen-oxidizing chemolithoautotrophic Desulfovibrionaceae sp. identified phyla not typically associated with lineages Spirochaetota Bacteroidota. Highly abundant sulfur-reducing Desulfuromusa transcribed non-coding RNAs transcriptional regulation, showing potential evidence metabolic flexibility response substrate availability. Despite low oxygen availability, oxidation primarily attributed aerobic Halothiobacillaceae. Low abundance transcription photoautotrophs indicated sulfur-based chemolithoautotrophy drives primary productivity even during periods constant illumination.We surficial chemolithoautotrophic, unique anoxic, cold, Arctic spring. We detected Mars-relevant metabolisms including hydrogenotrophic reduction, sulfur oxidation, which indicate for life analogous S-rich past present Mars. Video Abstract.
Language: Английский
Citations
14
Environmental Pollution, Journal Year: 2023, Volume and Issue: 335, P. 122303 - 122303
Published: Aug. 7, 2023
Language: Английский
Citations
13