The Klebsiella pneumoniae tellurium resistance gene terC contributes to both tellurite and zinc resistance

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

ABSTRACT Klebsiella pneumoniae is widely recognized as a pathogen responsible for hospital-acquired infections and community-acquired invasive infections. It has rapidly become significant global public health threat due to the emergence of hypervirulent multidrug-resistant strains, which have increased challenges associated with treating life-threatening Tellurium resistance genes are widespread on virulence plasmids in K. isolates. However, core function ter operon ( terZABCDEF ) remains unclear. In this study, P1927 strain was isolated from sputum hospitalized pneumonia patient. The operon, along antimicrobial genes, identified large hybrid plasmid P1927. We generated terC deletion mutant demonstrated that exhibited reduced Galleria mellonella larva infection model. Further physiological functional analysis revealed not only important Te(IV) but also Zn(II), Mn(II), phage infection. All were highly inducible by stronger inducer than Te(IV), terBCDE induced Mn(II). Collectively, our study demonstrates novel functions TerC Zn(II) . IMPORTANCE health. Although clinical isolates, its been proposed proteins encoded form multi-site metal-binding complex, exact still unknown. TerC, central component tellurium determinant, previously shown interact outer membrane OmpA KpsD Escherichia coli , suggesting potential changes structure properties. Here, we report confers infection, be strong operon. Furthermore, factor. Taken together, results expand understanding role providing deeper insights into link between heavy metal(loid) determinants pathogenic bacteria.

Language: Английский

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A riboswitch-controlled TerC family transporter Alx tunes intracellular manganese concentration in Escherichia coli at alkaline pH

Journal of Bacteriology, Journal Year: 2024, Volume and Issue: 206(7)

Published: June 13, 2024

Cells use transition metal ions as structural components of biomolecules and cofactors in enzymatic reactions, making integral cellular components. Organisms optimize ion concentration to meet needs by regulating the expression proteins that import export ion, often a concentration-dependent manner. One such regulation mechanism is via riboswitches, which are 5'-untranslated regions an mRNA undergo conformational changes promote or inhibit downstream gene, commonly response ligand. The

Language: Английский

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Metals in Motion: Understanding Labile Metal Pools in Bacteria

Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Metal ions are essential for all life. In microbial cells, potassium (K+) is the most abundant cation and plays a key role in maintaining osmotic balance. Magnesium (Mg2+) dominant divalent required nucleic acid structure as an enzyme cofactor. Microbes typically require transition metals manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), although precise set of metal needed to sustain life variable. Intracellular pools can be conceptualized chemically complex mixture rapidly exchanging (labile) ions, complemented by those reservoirs that exchange slowly relative cell metabolism (sequestered). Labile buffered transient interactions with anionic metabolites macromolecules, ribosome playing major role. Sequestered include many metalloproteins, cofactors, storage depots, some redeployed upon depletion. Here, I review size, composition, dynamics intracellular highlight gaps understanding.

Language: Английский

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The TerC family metal chaperone MeeY enables surfactin export in Bacillus subtilis

Journal of Bacteriology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

ABSTRACT TerC family proteins are widely conserved integral membrane with functions related to metal transport. In Bacillus subtilis , the MeeF and MeeY play overlapping roles in metalation of manganese-requiring extracellular enzymes. interact secretion translocon SecYEG metalate either during or after protein translocation. Here, we demonstrate that swarming motility is dependent on MeeY. This defect can be complemented extracellularly correlated a loss surfactin. Surfactin export mediated by SwrC, an RND efflux pump previously shown co-immunoprecipitation studies. The amendment growth medium manganese has long been known enhance surfactin production. We suggest model which enhanced MeeY-dependent lipopeptide export. IMPORTANCE produces surfactin, powerful detergent-like compound intercellular communication, surface motility, as broad-spectrum antimicrobial agent. Production cyclic lipopeptide, depends non-ribosomal peptide synthase followed member resistance-nodulation-cell division (RND) proteins. production additionally requires MeeY, exports ions support function secreted metalloenzymes. propose interacts SwrC facilitate binding from cell. These results may explain long-appreciated role divalent industrial fermentation.

Language: Английский

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Metalation of Extracytoplasmic Proteins and Bacterial Cell Envelope Homeostasis

Annual Review of Microbiology, Journal Year: 2024, Volume and Issue: 78(1), P. 83 - 102

Published: July 4, 2024

Cell physiology requires innumerable metalloenzymes supported by the selective import of metal ions. Within crowded cytosol, most enzymes acquire their cognate cofactors from a buffered labile pool. Metalation membrane-bound and secreted exoenzymes is more problematic since concentrations are highly variable outside cell. Here, we focus on involved in cell envelope homeostasis. Peptidoglycan synthesis often relies Zn-dependent hydrolases, metal-dependent β-lactamases play important roles antibiotic resistance. In gram-positive bacteria, lipoteichoic acid Mn, with TerC family Mn exporters supporting role. For some exoenzymes, metalation occurs metalated exported through TAT secretion system. others, facilitated exporters, metallochaperones, or partner proteins that enhance affinity. To help ensure function, can function multiple metals. Thus, cells employ diversity strategies to functioning cytosol.

Language: Английский

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How bacteria actively use passive physics to make biofilms

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(40)

Published: Sept. 12, 2024

Modern molecular microbiology elucidates the organizational principles of bacterial biofilms via detailed examination interplay between signaling and gene regulation. A complementary biophysical approach studies mesoscopic dependencies at cellular multicellular levels with a distinct focus on intercellular forces mechanical properties whole biofilms. Here, motivated by recent advances in biofilm research other, seemingly unrelated fields biology physics, we propose perspective that links biofilm, dynamic organism, physical processes occurring extracellular milieu. Using Bacillus subtilis as an illustrative model specifically demonstrate how such rationale explains architecture, differentiation, communication, stress responses desiccation tolerance, metabolism, physiology across multiple scales—from matrix proteins polysaccharides to macroscopic wrinkles water-filled channels.

Language: Английский

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Disruption of the bacterial OLE RNP complex impairs growth on alternative carbon sources

PNAS Nexus, Journal Year: 2024, Volume and Issue: 3(2)

Published: Feb. 1, 2024

Abstract Ornate, large, extremophilic (OLE) RNAs comprise a class of large noncoding in bacteria whose members form membrane-associated ribonucleoprotein (RNP) complex. This complex facilitates cellular adaptation to diverse stresses such as exposure cold, short-chain alcohols, and elevated Mg2+ concentrations. Here, we report additional phenotypes exhibited by Halalkalibacterium halodurans (formerly called Bacillus halodurans) strains lacking functional OLE RNP complexes. Genetic disruption the causes restricted growth compared wild-type cells when cultured minimal media (MM) wherein glucose is replaced with alternative carbon/energy sources. suppressor selections conducted glutamate MM yielded isolates that carry mutations or near genes relevant Mn2+ homeostasis (ykoY mntB), phosphate (phoR), putative multidrug resistance (bmrCD). These links between RNA, management, other fundamental processes including protein secretion are consistent hypothesis major contributor unfavorable conditions.

Language: Английский

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The Bacillus subtilis yqgC-sodA operon protects magnesium-dependent enzymes by supporting manganese efflux

Journal of Bacteriology, Journal Year: 2024, Volume and Issue: 206(6)

Published: May 31, 2024

Microbes encounter a myriad of stresses during their life cycle. Dysregulation metal ion homeostasis is increasingly recognized as key factor in host-microbe interactions. Bacterial tightly regulated by dedicated metalloregulators that control uptake, sequestration, trafficking, and efflux. Here, we demonstrate deletion the

Language: Английский

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Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Mobile genetic elements (MGEs) like plasmids, viruses, and transposable can provide fitness benefits to their hosts for survival in the presence of environmental stressors. Heavy metal resistance genes (HMRGs) are frequently observed on MGEs, suggesting that MGEs may be an important driver adaptive evolution environments contaminated with heavy metals. Here, we report meta-mobilome metal-contaminated regions Oak Ridge Reservation subsurface. This was compared one derived from samples collected unimpacted We assembled 1615 unique circularized DNA propose MGEs. The circular highly subsurface were enriched HMRG clusters relative those nearby regions. Additionally, found these HMRGs associated Gamma Betaproteobacteria potentially facilitate persistence dominance taxa this region. Finally, conjugative elements, potential future lateral transfer. demonstrate how our understanding MGE ecology, evolution, function enhanced through genomic context provided by completed assemblies.

Language: Английский

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Bioinformatic prediction of proteins relevant to functions of the bacterial OLE ribonucleoprotein complex

mSphere, Journal Year: 2024, Volume and Issue: 9(6)

Published: May 21, 2024

OLE (ornate, large, extremophilic) RNAs are members of a noncoding RNA class present in many Gram-positive, extremophilic bacteria. The large size, complex structure, and extensive sequence conservation characteristics consistent with the hypothesis that they likely function as ribozymes. representative from

Language: Английский

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