A contextual fear conditioning paradigm in head-fixed mice exploring virtual reality. DOI Creative Commons
Seetha Krishnan, Can Dong,

Heather Ratigan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

ABSTRACT Contextual fear conditioning is a classical laboratory task that tests associative memory formation and recall. Techniques such as multi-photon microscopy holographic stimulation offer tremendous opportunities to understand the neural underpinnings of these memories. However, techniques generally require animals be head-fixed. There are few paradigms test contextual in head-fixed mice, none where behavioral outcome following freezing, most common measure freely moving animals. To address this gap, we developed paradigm mice using virtual reality (VR) environments. We designed an apparatus deliver tail shocks (unconditioned stimulus, US) while navigated VR environment (conditioned CS). The acquisition was tested when were reintroduced shock-paired day. three different variations and, all them, observed increased conditioned response characterized by freezing behavior. This especially prominent during first trial environment, compared neutral received no shocks. Our results demonstrate can VR, discriminate between feared context, display response, similar behaving Furthermore, two-photon microscope, imaged from large populations hippocampal CA1 neurons before, during, conditioning. findings reconfirmed those literature on animals, showing place cells undergo remapping show narrower fields approach offers new study mechanisms underlying formation, recall, extinction As preparation compatible with stimulation, it enables long-term tracking manipulation throughout distinct stages provides subcellular resolution for investigating axonal, dendritic, synaptic dynamics real-time.

Language: Английский

Projections from thalamic nucleus reuniens to hippocampal CA1 area participate in context fear extinction by affecting extinction-induced molecular remodeling of excitatory synapses DOI Creative Commons
Magdalena Ziółkowska, Narges Sotoudeh, Anna Cały

et al.

eLife, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

The ability to extinguish contextual fear in a changing environment is crucial for animal survival. Recent data support the role of thalamic nucleus reuniens (RE) and its projections dorsal hippocampal CA1 area (RE→dCA1) this process. However, it remains poorly understood how RE impacts dCA1 neurons during extinction (CFE). Here, we reveal that RE→dCA1 pathway contributes by affecting CFE-induced molecular remodeling excitatory synapses. Anatomical tracing chemogenetic manipulation mice demonstrate form synapses regulate synaptic transmission stratum oriens (SO) lacunosum-moleculare (SLM) area, but not radiatum (SR). We also observe CFE-specific structural changes expression scaffold protein, PSD-95, both strata innervated RE, SR. Interestingly, only SLM are specific dendrites RE. To further projection CFE, brief inhibition CFE session persistently impairs formation memory PSD-95 levels SLM. Thus, our indicate participates regulating

Language: Английский

Citations

0
Projections from thalamic nucleus reuniens to hippocampal CA1 area participate in context fear extinction by affecting extinction-induced molecular remodeling of excitatory synapses DOI Open Access
Magdalena Ziółkowska, Narges Sotoudeh, Anna Cały

et al.

Published: Oct. 8, 2024

The ability to extinguish contextual fear in a changing environment is crucial for animal survival. Recent data support the role of thalamic nucleus reuniens (RE) and its projections dorsal hippocampal CA1 area (RE→dCA1) this process. However, it remains poorly understood how RE impacts dCA1 neurons during extinction (CFE). Here, we reveal that RE→dCA1 pathway contributes consolidation CFE memory by affecting CFE-induced molecular remodeling excitatory synapses. Anatomical tracing chemogenetic manipulation mice demonstrate form synapses regulate synaptic transmission stratum oriens (SO) lacunosum-moleculare (SLM) area, but not radiatum (SR). We also observe CFE-specific structural changes expression scaffold protein, PSD-95, both strata innervated RE, SR. Interestingly, only SLM are specific dendrites RE. To further projection CFE, brief inhibition session persistently impairs formation PSD-95 levels SLM. Thus, our indicate participates regulating

Language: Английский

Citations

0
Ventral hippocampal interneurons govern extinction and relapse of contextual associations DOI Creative Commons
Anthony F. Lacagnina, Tri Dong,

Rasika R. Iyer

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(11), P. 114880 - 114880

Published: Oct. 18, 2024

Language: Английский

Citations

0
Prefrontal projections to the bed nuclei of the stria terminalis modulate the specificity of aversive memories DOI Creative Commons
Jason J. Radley, Ryan T. Lingg, Shane B. Johnson

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Generalizing aversive memories helps organisms avoid danger, whereas discriminating between dissimilar situations promotes opportunistic behaviors. We identified a novel pathway that controls the contextual specificity of memory consolidation inhibitory avoidance learning. Optogenetic inhibition rostral medial prefrontal cortex (mPFC)-to-anteroventral bed nuclei stria terminalis (avBST) after single footshock exacerbated stress hormonal output, and 2 d later promoted generalization to context. Rostral mPFC-avBST influences were directly mnemonic rather than associated with hormone increases, as adrenalectomy did not prevent such on generalization. next observed fear discrimination contexts engaged activity along mPFC avBST pathway. Finally, post-footshock optogenetic excitation enhanced 2-d discrimination. These findings highlight in which immediately experiences threatening non-threatening may be importance for understanding trauma psychiatric illnesses.

Language: Английский

Citations

0
A contextual fear conditioning paradigm in head-fixed mice exploring virtual reality. DOI Creative Commons
Seetha Krishnan, Can Dong,

Heather Ratigan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

ABSTRACT Contextual fear conditioning is a classical laboratory task that tests associative memory formation and recall. Techniques such as multi-photon microscopy holographic stimulation offer tremendous opportunities to understand the neural underpinnings of these memories. However, techniques generally require animals be head-fixed. There are few paradigms test contextual in head-fixed mice, none where behavioral outcome following freezing, most common measure freely moving animals. To address this gap, we developed paradigm mice using virtual reality (VR) environments. We designed an apparatus deliver tail shocks (unconditioned stimulus, US) while navigated VR environment (conditioned CS). The acquisition was tested when were reintroduced shock-paired day. three different variations and, all them, observed increased conditioned response characterized by freezing behavior. This especially prominent during first trial environment, compared neutral received no shocks. Our results demonstrate can VR, discriminate between feared context, display response, similar behaving Furthermore, two-photon microscope, imaged from large populations hippocampal CA1 neurons before, during, conditioning. findings reconfirmed those literature on animals, showing place cells undergo remapping show narrower fields approach offers new study mechanisms underlying formation, recall, extinction As preparation compatible with stimulation, it enables long-term tracking manipulation throughout distinct stages provides subcellular resolution for investigating axonal, dendritic, synaptic dynamics real-time.

Language: Английский

Citations

0