Rapid Adaption but Genetic Diversity Loss of a Globally Distributed Diatom in the Warmer Ocean DOI

Luman Cheng,

Xiuming Xu, Minghua Wang

et al.

Global Change Biology, Journal Year: 2024, Volume and Issue: 30(12)

Published: Dec. 1, 2024

ABSTRACT Studies have demonstrated that marine phytoplankton can adapt to the warmer environment. However, underlying mechanisms remain largely unknown. Here, we quantified capacity of a globally distributed diatom Skeletonema dohrnii , for rapid evolution under moderate (24°C) and severe (28°C) warming scenarios. Whole‐genome resequencing analysis revealed evolutionary adaptation S. was slow (i.e., 700 generations), whereas it 300 generations) but suffered substantial loss genetic diversity within population. Genes associated with energy production lipid metabolism evolved rapidly, particularly warming, suggesting their vital roles in thermal adaptation. Proteomic results also showed enhanced expression proteins involved metabolism, especially warming. Furthermore, particulate organic carbon nitrogen greatly warming‐selected population increased insignificantly population, indicating more driven by Our provide molecular insights into limited diatoms highlight as most important adaptive strategy. Future will affect dynamics ocean.

Language: Английский

Eukaryotic phytoplankton drive a decrease in primary production in response to elevated CO 2 in the tropical and subtropical oceans DOI Creative Commons
Rongbo Dai, Zuozhu Wen, Haizheng Hong

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(11)

Published: March 10, 2025

Ocean acidification caused by increasing anthropogenic CO 2 is expected to impact marine phytoplankton productivity, yet the extent and even direction of these changes are not well constrained. Here, we investigate responses community composition productivity across western North Pacific. Consistent reductions in primary production were observed under acidified conditions Pacific Subtropical Gyre northern South China Sea, whereas no significant found at boundary subtropical gyre. While prokaryotic showed little or positive high , small (<20 µm) eukaryotic which primarily limited low ambient nitrogen drove decrease production. Extrapolating results global tropical oceans predicts a potential about 5 Pg C y −1 Chl- oligotrophic regions, anticipated experience both stratification future.

Language: Английский

Citations

0
Nitroxoline evidence Amoebicidal Activity against Acanthamoeba castellanii through DNA damage and the stress response pathways DOI Creative Commons
Lijun Chen, Wei Han, Wenwen Jing

et al.

International Journal for Parasitology Drugs and Drug Resistance, Journal Year: 2025, Volume and Issue: 27, P. 100578 - 100578

Published: Jan. 5, 2025

Acanthamoeba castellanii is a widespread unicellular eukaryote found in diverse environments, including tap water, soil, and swimming pools. It responsible for severe infections, such as keratitis granulomatous amebic encephalitis, particularly individuals with immunocompromisation. The ability of protozoans to form dormant persistent cysts complicates treatment, current therapies are ineffective against cyst stages suffer from poor specificity side effects. Nitroxoline, quinoline derivative well-established antibacterial, antifungal, antiviral properties, promising therapeutic candidate. This study aimed elucidate cellular signalling events that counteract the effects nitroxoline. In this study, nitroxoline significantly reduced viability A. trophozoites dose- time-dependent manner, inducing morphological changes apoptosis. Transcriptomic analysis revealed substantial alterations gene expression, enrichment metabolic pathways, DNA damage responses, iron ion binding. Nitroxoline treatment upregulated genes involved repair oxidative stress response while regulating methionine cysteine cycles. also decreased mitochondrial membrane potential, H₂S production, total amount castellanii. Bioinformatic analyses molecular docking studies suggest direct interactions between several proteins. Our research provides comprehensive map nitroxoline, revealing significant expression related pathways. These findings underscore potential potent anti-Acanthamoeba agent, offering new insights into its mechanism action paving way effective combinational strategies.

Language: Английский

Citations

0
Molecular Mechanisms for Iron Uptake and Homeostasis in Marine Eukaryotic Phytoplankton DOI
Robert Lampe, Tyler H. Coale, Jeffrey B. McQuaid

et al.

Annual Review of Microbiology, Journal Year: 2024, Volume and Issue: 78(1), P. 213 - 232

Published: July 17, 2024

The micronutrient iron is essential for phytoplankton growth due to its central role in a wide variety of key metabolic processes including photosynthesis and nitrate assimilation. As result scarce bioavailable seawater, marine primary productivity often iron-limited with future supplies remaining uncertain. Although evolutionary constraints resulted high cellular requirements, evolved diverse mechanisms that enable uptake multiple forms iron, storage over short long timescales, modulation their requirement under stress. Genomics continues increase our understanding iron-related proteins are homologous those characterized other model organisms, while recently, molecular cell biology have been revealing unique genes connections acquisition or use. Moreover, there an increasing number examples showing the interplay between extracellular such as boundary layer chemistry microbial interactions.

Language: Английский

Citations

1
Iron limitation differentially affects viral replication in key marine microbes DOI
Charmaine C. M. Yung, Rachel L. Kelly, Kathryn M. Kauffman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 22, 2024

Abstract Viral lysis accounts for much of microbial mortality in the ocean, and iron (Fe) is a critical micronutrient that can limit phytoplankton growth, yet interactions between Fe-nutrition viral are not well known. Here, we present infection dynamics under Fe-limited Fe-replete conditions three distinct marine microbes, photosynthetic picoeukaryote Ostreococcus lucimarinus , cyanobacterium Synechococcus two strains heterotrophic bacterium Vibrio . Iron limitation resulted slowed corresponding decrease burst sizes was observed; this similar to results from studies larger eukaryotic (Slagter et al. 2016; Kranzler 2021), where reduced replication Fe-limitation attributed reliance on host metabolism machinery. For one strain similarly impacted dynamics, increasing latent period before infected cells release new virus, reducing number infective particles released upon lysis. Unexpectedly, another had no discernible effect replication. Furthermore, cyanophages affected by host, either terms or size. The illuminate extraordinary ability some viruses, particularly cyanophages, highjack produce particles, even when growth compromised. This has implications ecology carbon cycling regions global ocean.

Language: Английский

Citations

1
The North Pacific Eukaryotic Gene Catalog of metatranscriptome assemblies and annotations DOI Creative Commons
Ryan D. Groussman, Sacha Coesel, Bryndan P. Durham

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 22, 2024

Marine microbial eukaryotes (protists) perform essential metabolic functions in oceanic ecosystems. The diversity of protist remains poorly understood as few species have been isolated laboratory settings. Metatranscriptomes provide an invaluable tool for exploring and genetic capacities within their natural habitats. Here, we introduce the North Pacific Eukaryotic Gene Catalog, a compilation metatranscriptome data derived from total 261 metatranscriptomes: 169 metatranscriptomes were samples collected on three meridional surface transects along 158°W, each spanning ~20 degrees latitude Subtropical Gyre (NPSG) to Transition Zone (NPTZ); 92 two diel-resolved field studies, one NPSG at 157°W, 23°N, NPTZ 41°N. sequences de novo assembled into 175 assemblies pooled five datasets containing between 22 M 49 contigs clustered 99% protein identity. Assemblies annotated by taxonomy function, enumerated short read alignment. All are available Zenodo repository, with underlying code github.

Language: Английский

Citations

1
Siberian Greening Enhances Coastal Spring Chlorophyll in Western North America DOI Creative Commons
Young‐Min Yang, Jae‐Heung Park,

Mi-Kyung Sung

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Abstract Climate change is driving unprecedented changes in terrestrial and marine ecosystems, with profound effects on global atmospheric dynamics, carbon cycling, productivity1,2. In particular, Siberia has experienced an earlier onset of the growing season3,4,5 increased productivity4, contributing to regional warming4,5,6 altered aerosol emissions7,8. At same time, coastal spring chlorophyll northwestern US Canada undergoing significant due upwelling resulting nutrient enrichment, affecting ecological dynamics fisheries sustainability9–15. However, trends its future remain uncertain. This study shows a increasing trend along west North America, associated enhanced northerly winds induced by Siberian greening under greenhouse gas warming. Increased warming Siberia, greening, induces positive pressure northwest coast America during spring. These drive upwelling, leading elevated nitrate concentrations blooms. Model simulations incorporating current CO2 forcings consistently confirm observed increase levels waters northern California, Oregon, Washington, southern British Columbia. underscores central role shaping Northeastern Pacific Ocean ecosystem. The results highlight complex linkages between teleconnections, ecosystems. A comprehensive understanding these critical for predicting managing impacts gas-induced fish catches broader ecosystem dynamics. research will help refine predictions develop strategies mitigate broad climate

Language: Английский

Citations

0
Rapid Adaption but Genetic Diversity Loss of a Globally Distributed Diatom in the Warmer Ocean DOI

Luman Cheng,

Xiuming Xu, Minghua Wang

et al.

Global Change Biology, Journal Year: 2024, Volume and Issue: 30(12)

Published: Dec. 1, 2024

ABSTRACT Studies have demonstrated that marine phytoplankton can adapt to the warmer environment. However, underlying mechanisms remain largely unknown. Here, we quantified capacity of a globally distributed diatom Skeletonema dohrnii , for rapid evolution under moderate (24°C) and severe (28°C) warming scenarios. Whole‐genome resequencing analysis revealed evolutionary adaptation S. was slow (i.e., 700 generations), whereas it 300 generations) but suffered substantial loss genetic diversity within population. Genes associated with energy production lipid metabolism evolved rapidly, particularly warming, suggesting their vital roles in thermal adaptation. Proteomic results also showed enhanced expression proteins involved metabolism, especially warming. Furthermore, particulate organic carbon nitrogen greatly warming‐selected population increased insignificantly population, indicating more driven by Our provide molecular insights into limited diatoms highlight as most important adaptive strategy. Future will affect dynamics ocean.

Language: Английский

Citations

0