Emergence and spread of the SARS-CoV-2 omicron (BA.1) variant across Africa: an observational study

The Lancet Global Health, Journal Year: 2025, Volume and Issue: 13(2), P. e256 - e267

Published: Jan. 29, 2025

Language: Английский

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Assessment of sewer connectivity in the United States and its implications for equity in wastewater-based epidemiology

PLOS Global Public Health, Journal Year: 2024, Volume and Issue: 4(4), P. e0003039 - e0003039

Published: April 17, 2024

Wastewater-based epidemiology is a promising public health tool that can yield more representative view of the population than case reporting. However, only about 80% U.S. connected to sewers, and characteristics populations missed by wastewater-based are unclear. To address this gap, we used publicly available datasets assess sewer connectivity in location, demographic groups, economic groups. Data from Census’ American Housing Survey revealed was lower average when head household Indian Alaskan Native, White, non-Hispanic, older, for larger households those with higher income, but smaller geographic scales local variations national pattern. For example, data Environmental Protection Agency showed positively correlated income Minnesota, Florida, California. Community also areas low connectivity, such as Alaska, Navajo Nation, Michigan, Florida. exception Census data, there were inconsistencies across datasets. Using mathematical modeling impact wastewater sampling inequities on inferences epidemic trajectory at scale, found some situations, even weak connections between communities may allow monitoring one community serve reliable proxy an interacting no monitoring, cases widespread. A systematic, rigorous assessment will be important ensuring equitable informed implementation system.

Language: Английский

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Aptamer Paper-Based Fluorescent Sensor for Determination of SARS-CoV-2 Spike Protein

Sensors, Journal Year: 2025, Volume and Issue: 25(6), P. 1637 - 1637

Published: March 7, 2025

Point-of-care (POC) antigen detection plays a crucial role in curbing the spread of viruses. Paper-based fluorescence aptasensors are expected to offer low-cost tool meet needs decentralized POC diagnosis. Herein, we report on fluorescent paper-based sensing system for detecting SARS-CoV-2 spike protein. The was constructed by loading multi-layer Nb2C MXene nano-quenchers and carbon-dot-labeled aptamer (G-CDs@Apt) probes onto mixed cellulose ester (MCE) paper substrate. On MXene/G-CDs@Apt paper, abundant G-CDs@Apt were attached multilayer kept fluorescence-off state, while recognition target detached formed nano--quenchers, resulting recovery paper. developed sensor performed well one-step S1 protein with limit 0.067 ng/mL (0.335 pg/test). assay exhibited good selectivity anti-interference artificial saliva. Moreover, aptasensor successfully used detect actual environmental samples recoveries 90.87–100.55% relative standard deviations 1.52–3.41%. proposed technology provides cost-effective alternative traditional antibody test strips wide range diagnostic applications.

Language: Английский

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Immunological drivers of zoonotic virus emergence, evolution, and endemicity

Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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Dynamics of SARS-CoV-2 Mutations in Wastewater Provide Insights into the Circulation of Virus Variants in the Population

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4324 - 4324

Published: May 1, 2025

SARS-CoV-2 high transmission and genomic mutations result in the emergence of new variants that impact COVID-19 vaccine efficacy virus by evading host immune system. Wastewater-based epidemiology is an effective approach to monitor circulation population but a challenge due presence reaction inhibitors low concentrations this environment. Here, we aim improve variant detection wastewater employing nested PCR followed next-generation sequencing (NGS) small amplicons S gene. Eight samples from Alegria Wastewater Treatment Plant, Rio de Janeiro, Brazil, were collected monthly February September 2021. Samples submitted concentration, RNA extraction NGS. The used prepare libraries for without need perform any fragmentation step. We identified calculated frequencies 29 matching Alpha, Beta, Gamma, Delta, Omicron, P.2 variants. Omicron matching-mutations detected before lineage was classified as concern. sequences clustered with clinical circulated 2021 Janeiro. show selected gene allows identification their frequencies’ calculation. This may be expanded using customizing primers additional regions, order differentiate current Approaches allow us learn how emerge they relate outcomes are crucial our understanding dynamics circulation, providing valuable data public health management.

Language: Английский

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Occurrence of plasmid pBI143 in wastewater in Baltimore

Published: May 1, 2025

Language: Английский

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Combining Short- and Long-Read Sequencing Technologies to Identify SARS-CoV-2 Variants in Wastewater

Viruses, Journal Year: 2024, Volume and Issue: 16(9), P. 1495 - 1495

Published: Sept. 21, 2024

During the COVID-19 pandemic, monitoring of SARS-CoV-2 RNA in wastewater was used to track evolution and emergence variant lineages gauge infection levels community, informing appropriate public health responses without relying solely on clinical testing. As more sublineages were discovered, it increased difficulty identifying distinct variants a mixed population sample, particularly those known lineage. Here, we compare sequencing technology from Illumina Oxford Nanopore Technologies, order determine their efficacy at detecting differing abundance, using 248 samples various Quebec Ontario cities. Our study two analytical approaches identify main samples: presence signature marker mutations co-occurrence within same amplicon. We observed that each method detected certain different frequencies as preferentially detects variants. with predominant lineage is low abundance across or unknown for time period, while had higher detection rate are predominantly found high B.1.1.7 (Alpha) well co-occurring present workflow integrates short-read long-read improve samples, such wastewater.

Language: Английский

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Characterisation of greenhouse gas emissions from temporary wastewater treatment plants under different operation modes

Process Safety and Environmental Protection, Journal Year: 2025, Volume and Issue: unknown, P. 107110 - 107110

Published: April 1, 2025

Language: Английский

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Cost-effectiveness of wastewater-based environmental surveillance for SARS-CoV-2 in Blantyre, Malawi and Kathmandu, Nepal: A model-based study

PLOS Global Public Health, Journal Year: 2025, Volume and Issue: 5(4), P. e0004439 - e0004439

Published: April 24, 2025

Wastewater-based environmental surveillance (ES) has been demonstrated to provide an early warning signal predict variant-driven waves of pathogens such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Our study evaluated the potential cost-effectiveness ES for SARS-CoV-2 compared with clinical testing alone. We used Covasim agent-based model COVID-19 simulate disease transmission hypothetical populations in Blantyre, Malawi, and Kathmandu, Nepal. simulated introduction a new immune-escaping variant over 6 months estimated health outcomes (cases, deaths, disability-adjusted life years [DALYs]) economic impact when using trigger moderate proactive behavioral intervention (e.g., increased use masks, social distancing) by policymakers versus no hence delayed reactive intervention. Costs considered included ES, testing, treatment, productivity loss entire population due implementation calculated incremental ratios these local willingness-to-pay thresholds: $61 Malawi $249 performed sensitivity analyses evaluate key assumptions on results. are reported 2022 US dollars. estimate that if were implemented, approximately 600 DALYs would be averted Blantyre 300 six-month period. Considering system costs, was cost-effective cost-saving Kathmandu. Cost-effectiveness highest settings low surveillance, high severity, effectiveness. However, from societal perspective, may not depending magnitude population-wide losses associated threshold. or tool perspective linked effective public response. From however, length its consequences make cost-effective. Implementing multiple improve cost-effectiveness.

Language: Английский

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Wastewater sequencing as a powerful tool to reveal SARS-CoV-2 variant introduction and spread in French Guiana, South America

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 924, P. 171645 - 171645

Published: March 11, 2024

The origin of introduction a new pathogen in country, the evolutionary dynamics an epidemic within and role cross-border areas on remain complex to disentangle are often poorly understood. For instance, represent ideal location for sharing viral variants between countries, with international air travel, land travel waterways playing important spread infectious diseases. Unfortunately, monitoring point entry viruses space time local populations challenging. Here we tested efficiency wastewater-based epidemiology genotyping Covid-19 SARS-CoV-2 variant French Guiana, tropical country located South America. Our results suggest that powerful tools monitor disease evolution but inclusion both clinical wastewater samples could still improve our understanding genetic diversity co-circulating. Wastewater sequencing also revealed cryptic transmission country. Interestingly, found some amino acid changes specific co-circulating suggesting after their introduction. More importantly, showed proximity bordering countries was not emergence Guianese B.1.160.25 variant, rather this emerged from ancestor B.1.160 introduced by European plane travelers, thus remains significant risk Overall, provides cost effective non-invasive approach early-warning tool

Language: Английский

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