Multiplex Magnetic Biosensor Microarrays Facilitate Personalized Vaccination Schedules for Sars-Cov-2 Variants DOI

Hyun-Kyung Um,

Songeun Kim,

Shan X. Wang

et al.

Published: Jan. 1, 2024

Language: Английский

Immunobiology of COVID-19: Mechanistic and therapeutic insights from animal models DOI Open Access

Hong‐Yi Zheng,

Tian‐Zhang Song,

Yong‐Tang Zheng

et al.

动物学研究, Journal Year: 2024, Volume and Issue: 45(4), P. 747 - 766

Published: Jan. 1, 2024

The distribution of the immune system throughout body complicates

Language: Английский

Citations

4
Immunity against conserved and non-conserved Spike epitopes after COVID-19 booster vaccination provides long-term protection against symptomatic Omicron infections DOI Creative Commons

Heiko Pfister,

Carsten Uhlig,

Zsuzsanna Mayer

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract The objective of this study was to investigate the features immune protection against SARS-CoV-2 infection in a single cohort during 6–17 months following booster immunization with an mRNA-based vaccine. results illustrate influence humoral and cellular immunity on efficacy Notably, neutralizing antibody titers were found serve as reasonably reliable correlate prior immunization. However, predictive power largely lost after boosting. loss appears be due critical remodeling response Our findings support hypothesis that both conserved non-conserved epitopes viral Spike protein's receptor-binding domain (RBD) is crucial for optimal long-term Omicron infection. While may provide cross-variant protection, antibodies targeting RBD play pivotal role achieving maximum protection. These observations highlight repeated shaping landscape reinforce necessity considering components, alongside intended use considerations, when assessing vaccine developing future strategies.

Language: Английский

Citations

0
Ultra-Sensitive Aptamer-Based Diagnostic Systems for Rapid Detection of All SARS-CoV-2 Variants DOI Open Access
Sang Won Kim,

Min Jung Han,

Md Shafiqur Rahman

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 745 - 745

Published: Jan. 16, 2025

The emergence of numerous SARS-CoV-2 variants, characterized by mutations in the viral RNA genome and target proteins, has presented challenges for accurate COVID-19 diagnosis. To address this, we developed universal aptamer probes capable binding to spike proteins including highly mutated strains like Omicron. These aptamers were identified through protein-based SELEX using from three key variants (D614G-substituted Wuhan-Hu-1, Delta, Omicron) virus-based SELEX, known as viro-SELEX. Leveraging these aptamers, created a sensitive lateral flow assay (LFA) an ultra-sensitive molecular diagnostic platform that integrates novel rapid PCR technique, enabling fast reliable detection across all variants.

Language: Английский

Citations

0
Longitudinal immunogenicity cohort study of SARS-CoV-2 mRNA vaccines across individuals with different immunocompromising conditions: heterogeneity in the immune response and crucial role of Omicron-adapted booster doses DOI Creative Commons
Annalisa Ciabattini,

Elena Pettini,

Fabio Fiorino

et al.

EBioMedicine, Journal Year: 2025, Volume and Issue: 113, P. 105577 - 105577

Published: Feb. 4, 2025

Language: Английский

Citations

0
Precision mapping of the functional epitope of a SARS-CoV-2 neutralizing antibody via hydrogen–deuterium exchange mass spectrometry DOI
Ji Woong Kim, In Young Ko,

Ha Gyeong Shin

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113187 - 113187

Published: Feb. 1, 2025

Language: Английский

Citations

0
Pan‐Variant SARS‐CoV‐2 Vaccines Induce Protective Immunity by Targeting Conserved Epitopes DOI Creative Commons

Masaud Shah,

Sung Ung Moon,

Ji‐Yon Shin

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Abstract The development of a globally effective COVID‐19 vaccine faces significant challenges, particularly in redirecting the B‐cell response from immunodominant yet variable regions viral proteins toward their conserved domains. To address this, an integrated strategy is implemented that combines classical epitope prediction with protein‐antibody cluster docking and antibody titer analysis 30 vaccinated convalescent individuals. This approach yields stable immunoprevalent epitopes capable eliciting robust responses BALB/c mice effectively neutralizing pseudoviruses expressing Spike protein SARS‐CoV‐2 variants concern, including Alpha, Beta, Gamma, Delta, Omicron. achieve broader T‐cell‐based immune response, promiscuous T‐cell are identified by integrating predictions, differential scanning fluorimetry, peptide‐MHC structural analysis. Unique peptides MHC‐anchoring residues identified, enabling binding to spectrum MHC‐I MHC‐II haplotypes. These elicit strong interferon gamma human peripheral blood mononuclear cells demonstrate cross‐species efficacy activating both CD4+ CD8+ T‐cells mice. Collectively, these findings highlight significance innovative strategies targeting immunodominant/immunoprevalent drive broad humoral cellular against wide range variants.

Language: Английский

Citations

0
SARS-CoV-2 Fusion Peptide-Directed Antibodies Elicited by Natural Infection Mediate Broad Sarbecovirus Neutralization DOI Creative Commons
Alex Roederer, Yi Cao,

Chia Jung Li

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

SUMMARY Studies have demonstrated that repeated mRNA vaccination enhances the breadth of neutralization against diverse SARS-CoV-2 variants. However, development antibodies capable neutralizing across Coronavirinae subfamily is poorly understood. In this study, we analyze serum samples to determine their and potency identify antigenic targets. Using a cohort older individuals healthcare workers, track correlates broad responses, including fusion peptide (FP) antibody elicitation. We find although broadly responses are often result RBD-specific antibodies, rare subset donors produce FP-specific responses. Interestingly, not observed in COVID-naive irrespective regimen, but rather, they occur following natural infection or vaccine breakthrough. This study highlights epitope targets underpinning coronaviruses suggests existing vaccines insufficient promote elicitation FP-directed coronavirus antibodies.

Language: Английский

Citations

0
Comparison of the ACE2 receptor and monoclonal antibodies immobilisation strategies for the sensitive detection of SARS-CoV-2 variants of concern DOI
Silvija Juciute, Vincentas Mačiulis, Asta Lučiūnaitė

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 344075 - 344075

Published: April 1, 2025

Language: Английский

Citations

0
Nanobody-based strategy for rapid and accurate pathogen detection: A case of COVID-19 testing DOI

Wenjin Hu,

Yichen Liu, Xi Li

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 263, P. 116598 - 116598

Published: July 22, 2024

Language: Английский

Citations

2
Structural review of SARS-CoV-2 antiviral targets DOI Creative Commons
Wen Cui, Yinkai Duan, Gao Yan

et al.

Structure, Journal Year: 2024, Volume and Issue: 32(9), P. 1301 - 1321

Published: Sept. 1, 2024

Language: Английский

Citations

2