Cited by YTHDF2 promotes arsenic-induced malignant phenotypes by degrading PIDD1 mRNA in human keratinocytes

Progress in understanding the regulatory mechanisms of immune checkpoint proteins PD-1 and PD-L1 expression DOI

Xuanxuan Wu,

Zengjun Zhu,

Jian Zhang

et al.

Clinical & Translational Oncology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Language: Английский

Citations

TBX3 shapes an immunosuppressive microenvironment and induces immunotherapy resistance DOI

Zhi Liu, Chunyu Zhang,

Jiatong Xiao

et al.

Theranostics, Journal Year: 2025, Volume and Issue: 15(5), P. 1966 - 1986

Published: Jan. 6, 2025

Background: Identifying biomarkers that predict immunotherapy efficacy and discovering new targets for combination therapies are critical elements improving the prognosis of bladder cancer (BLCA) patients. Methods: Firstly, we explored expression patterns TBX3 in normal pan-cancer tissues correlation between immune microenvironment using data from multiple public databases. Then, combined various techniques, including bulk RNA sequencing, single-cell high-throughput cytokine arrays, functional experiments, ProcartaPlex multiplex immunoassays TissueFAXS panoramic tissue quantification assays, to demonstrate shapes an immunosuppressive tumor (TME) BLCA. Results: We identified as a key factor associated with BLCA through systematic multi-omics analysis. found is primarily expressed malignant cells, where TBX3high cells increase secretion TGFβ1, which promotes infiltration cancer-associated fibroblasts (CAFs), thereby forming microenvironment. further demonstrated enhances TGFβ1 by binding promoter, blocking counteracts effects TBX3. Moreover, reduced cancer-killing efficiency CD8+ T decreasing proportion GZMB+ knocking down anti-PD-1 treatment increased cell CAFs vivo. also validated inverse relationship TBX3+ positive microarrays. Lastly, predicted our real-world cohort cohorts. Conclusion: In summary, progression resistance inducing microenvironment, targeting could enhance

Language: Английский

Citations

The coagulation and tumor system are directly linked through the proteolysis and activation of epidermal growth factor receptor by thrombin DOI

Tianfa Wang,

Zhiyuan Shen, Liu Yang

et al.

Oncogene, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Language: Английский

Citations

Integrative analysis of single-cell and bulk RNA sequencing reveals the oncogenic role of ANXA5 in gastric cancer and its association with drug resistance DOI

Denggang Chen,

Peng Zhang,

Li Gong

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Background Gastric cancer (GC) remains a leading cause of cancer-related mortality, with over one million new cases and 769,000 deaths reported in 2020. Despite advancements chemotherapy, surgery, targeted therapies, delayed diagnosis due to overlooked early symptoms leads poor prognosis. Methods We integrated bulk RNA sequencing single-cell datasets from TCGA, GEO, OMIX001073, employing normalization, batch effect correction, dimensionality reduction methods identify key cell populations associated GC invasion epithelial-mesenchymal transition (EMT), as well analyze the tumor immune microenvironment. Results Our analysis identified MUC5AC+ malignant epithelial cluster significant player EMT. Cluster 1, representing this population, exhibited higher EMT scores compared other clusters. Survival showed that high abundance 0 correlated improved survival rates (P=0.012), whereas 1 was poorer outcomes (P=0.045). A prognostic model highlighted ANXA5 GABARAPL2 two critical genes upregulated tumors. High-risk patients demonstrated increased infiltration worse prognosic. Analysis mutation burden (TMB) indicated low TMB high-risk group had worst Wet-lab validation experiments confirmed oncogenic role ANXA5, showing its facilitation proliferation, invasion, migration while suppressing apoptosis. Conclusion This study offers novel insights into subpopulations cells their roles progression. It provides potential therapeutic targets combat GC, contributing crucial understanding fundamental mechanisms drug resistance gastrointestinal cancers.

Language: Английский

Citations

Plac1⁺ Tumor Cell‐Treg Interplay Supports Tumorigenesis and Progression of Head and Neck Cancer DOI

Xiaoyan Meng,

Zhonglong Liu,

Lu Zhao

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Cancer/testis antigen (CTA) family is restricted to germline and tumor cells plays an important role during cancer initiation progression. Five single-cell two bulk RNA-seq datasets are integrated screen genes in the CTAs family, revealing that Placenta specific protein 1 (Plac1) specifically expressed head neck squamous cell carcinoma (HNSCC) cells. Sp1 Transcription (SP1) identified as a regulator of Plac1, which confirmed by cleavage under targets tagmentation (CUT&Tag)-seq. With vitro experiments, vivo subcutaneous tumor, transgenic autochthonous model, it revealed Plac1 expression promotes HNSCC progression inducing epidermal growth factor receptor endocytosis recycling increase PI3K/AKT signaling pathway activity. Then, Plac1+ recruit CD4+ T via CXCL11/CXCR3 induce Treg differentiation PVR/TIGIT, turn activates tumorigenic LTA/LTBR forms reciprocal protumor loop. These findings provide insights into molecular features facilitate development personalized treatment strategies.

Language: Английский

Citations

Construction of a circRNA-miRNA-mRNA Regulatory Network for the Immune Regulation of Lung Adenocarcinoma DOI

Hanyi Li, Xin Jin, Wei Li

et al.

Biological Procedures Online, Journal Year: 2025, Volume and Issue: 27(1)

Published: April 10, 2025

Recent research has highlighted the significance of circular RNAs (circRNAs) as pivotal regulators in progression tumors and therapeutic response non-small cell lung cancer (NSCLC). These circRNAs function through a sponge mechanism, interacting with microRNAs (miRNAs) to modulate mRNA expression levels. Nevertheless, precise role circRNA-miRNA-mRNA regulatory network immune regulation within adenocarcinoma (LUAD) remains inadequately understood. We utilized microarray datasets from GEO NCBI database (GSE101586) identify differentially expressed (DEcircRNAs) LUAD. CircBank was employed predict target miRNAs DEcircRNAs, which were subsequently intersected GSE36681 database. The identified then predicted mRNAs using miRDB miWalk, intersections immune-related genes IMMPORT analyzed. Protein-protein interaction (PPI) networks constructed Cytoscape software. DAVID functional annotation tool explore potential biological processes, molecular functions, KEGG pathways associated Gene Kaplan-Meier survival analyses conducted establish key assess infiltration Pearson correlation for significant genes. Finally, we selected most significantly upregulated circRNA differential validation vitro experiments. Our analysis total 7 42 downregulated circRNAs, along 10 20 mRNAs. enrichment indicated that these components are primarily enriched ErbB signaling pathway. Furthermore, Ontology (GO) revealed responses organic substances, cytokine-mediated pathways, cellular cytokines, chemical stimuli, steroid hormone receptor activity, ErbB-3 class binding, oxysterol signal transducer activity. Notable core included OAS1, VIPR1, PIK3R1. Subsequently, comprising 6 3 DEmiRNAs, DEmRNAs. Through ssGSEA CIBERSORT analyses, observed differences levels between NSCLC cohort control group. Knocking down hsa_circ_0079557 inhibited viability, proliferation, migration, invasion LUAD cells. have established offers novel insights into mechanisms governing Future should aim translate findings clinical applications enhance patient outcomes.

Language: Английский

Citations

FANCA promotes lung adenocarcinoma progression and is a potential target for epitope vaccine immunotherapy DOI

Yanli Kang,

Ruifang Zhong,

Yuhan Gan

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 7, 2024

FANCA mutations have been detected in a variety of cancers and found to be pro-carcinogenic. However, no functional studies identified regarding the involvement occurrence immune response LUAD.

Language: Английский

Citations

MAZ promotes tumor proliferation and immune evasion in lung adenocarcinoma DOI

Yan Chen,

Xinsheng Zhu,

Jue Wang

et al.

Oncogene, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Language: Английский

Citations

Machine learning-based discovery of UPP1 as a key oncogene in tumorigenesis and immune escape in gliomas DOI

Zigui Chen, Chao Liu, Chunyuan Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 24, 2024

Introduction Gliomas are the most common and aggressive type of primary brain tumor, with a poor prognosis despite current treatment approaches. Understanding molecular mechanisms underlying glioma development progression is critical for improving therapies patient outcomes. Methods The study comprehensively analyzed large-scale single-cell RNA sequencing bulk samples. By utilizing series advanced computational methods, this integrative approach identified gene UPP1 (Uridine Phosphorylase 1) as novel driver tumorigenesis immune evasion. Results High levels were linked to survival rates in patients. Functional experiments demonstrated that promotes tumor cell proliferation invasion suppresses anti-tumor responses. Moreover, was found be an effective predictor mutation patterns, drug response, immunotherapy effectiveness, characteristics. Conclusions These findings highlight power combining diverse machine learning methods identify valuable clinical markers involved pathogenesis. Identifying growth escape may promising therapeutic target devastating disease.

Language: Английский

Citations

Uridine Phosphorylase-1 supports metastasis of mammary cancer by altering immune and extracellular matrix landscapes of the lung DOI

Declan Whyte, Johan Vande Voorde, David Sumpton

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 4, 2024

Summary Understanding the mechanisms that facilitate early events in metastatic seeding is key to developing therapeutic approaches reduce metastasis – leading cause of cancer-related death. Using whole animal screens genetically engineered mouse models cancer we have identified circulating metabolites associated with metastasis. Specifically, highlight pyrimidine uracil as a prominent metastasis-associated metabolite. Uracil generated by neutrophils expressing enzyme uridine phosphorylase-1 (UPP1), and neutrophil specific Upp1 expression increased cancer. Altered UPP1 activity influences adhesion molecules on surface neutrophils, decreased motility pre-metastatic lung. Furthermore, find UPP1-expressing suppress T-cell proliferation, product can increase fibronectin deposition extracellular microenvironment. Consistently, knockout or inhibition mice mammary tumours increases number T-cells reduces content lung decreases proportion develop These data indicate behaviour matrix suggest pharmacological targeting this pathway could be an effective strategy

Language: Английский

Citations