Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study Results DOI Creative Commons
Lespek Kutumbetov, Balzhan Myrzakhmetova, Aiganym A. Tussipova

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(12), P. 1401 - 1401

Published: Dec. 12, 2024

The research conducted in this preclinical study assesses QazCovid-live, a live attenuated COVID-19 vaccine created Kazakhstan, by conducting evaluations of safety, immunogenicity, and allergenicity various animal models, including mice, rats, hamsters, guinea pigs. vaccine, developed attenuating SARS-CoV-2 via numerous Vero cell passages, had no significant adverse effects acute subacute toxicity assessments, even at elevated dosages. Allergenicity testing indicated the absence both immediate delayed hypersensitivity reactions. Immunogenicity revealed strong virus-neutralizing antibody responses, especially following intranasal intratracheal delivery. Studies on reversibility transmission further validated vaccine’s stability non-pathogenicity. data indicate that QazCovid-live is safe, immunogenic, prepared for clinical trials, presenting potential strategy prevention.

Language: Английский

Compartment-specific antibody correlates of protection to SARS-CoV-2 Omicron in macaques DOI Creative Commons
Xin Tong, Qixin Wang, Wonyeong Jung

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(9), P. 110174 - 110174

Published: June 5, 2024

Antibodies represent a primary mediator of protection against respiratory viruses. Serum neutralizing antibodies (NAbs) are often considered correlate protection. However, detailed antibody profiles including characterization functions in different anatomic compartments poorly understood. Here we show that correlates severe acute syndrome coronavirus 2 (SARS-CoV-2) challenge systemic versus mucosal rhesus macaques. In serum, NAbs were the strongest and linked to spike-specific binding other extra-NAb create larger protective network. bronchiolar lavage (BAL), antibody-dependent cellular phagocytosis (ADCP) proved rather than NAbs. Within BAL, ADCP was immunoglobulin (Ig)G, IgA/secretory IgA, Fcγ-receptor antibodies. Our results support model which with mediate at sites.

Language: Английский

Citations

1
The evolving landscape of live attenuated COVID-19 vaccines DOI
Simone Pecetta, Rino Rappuoli

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(8), P. 1911 - 1913

Published: Aug. 2, 2024

Language: Английский

Citations

1
T-Cell Immune Responses to SARS-CoV-2 Infection and Vaccination DOI Creative Commons
Samuele Notarbartolo

Vaccines, Journal Year: 2024, Volume and Issue: 12(10), P. 1126 - 1126

Published: Sept. 30, 2024

The innate and adaptive immune systems collaborate to detect SARS-CoV-2 infection, minimize the viral spread, kill infected cells, ultimately leading resolution of infection. system develops a memory previous encounters with virus, providing enhanced responses when rechallenged by same pathogen. Such immunological is basis vaccine function. Here, we review current knowledge on response infection vaccination, focusing pivotal role T cells in establishing protective immunity against virus. After an overview describe main features SARS-CoV-2-specific CD4

Language: Английский

Citations

1
Compartment-Specific Antibody Correlates of Protection to SARS-CoV-2 Omicron in Macaques DOI Creative Commons
Xin Tong, Qixin Wang, Wonyeong Jung

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 1, 2024

Abstract Antibodies represent a primary mediator of protection against respiratory viruses such as SARS-CoV-2. Serum neutralizing antibodies (NAbs) are often considered correlate protection. However, detailed antibody profiles including characterization functions in different anatomic compartments not well understood. Here we show that correlates SARS-CoV-2 challenge systemic versus mucosal rhesus macaques. In serum, were the strongest and linked to Spike-specific binding other extra-neutralizing create larger protective network. contrast, bronchiolar lavage (BAL), antibody-dependent cellular phagocytosis (ADCP) proved rather than NAbs. Within BAL, ADCP was IgG, IgA/secretory IgA, Fcγ-receptor antibodies. Our results support model which with mediate at sites. The correlation Fc functional responses BAL suggests these may be critical for Omicron mucosa.

Language: Английский

Citations

0
Navigating the Landscape of B Cell Mediated Immunity and Antibody Monitoring in SARS-CoV-2 Vaccine Efficacy: Tools, Strategies and Clinical Trial Insights DOI Creative Commons
Sophie O’Reilly, Joanne Byrne, Eoin R. Feeney

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(10), P. 1089 - 1089

Published: Sept. 24, 2024

Correlates of Protection (CoP) are biomarkers above a defined threshold that can replace clinical outcomes as primary endpoints, predicting vaccine effectiveness to support the approval new vaccines or follow up studies. In context COVID-19 vaccination, CoPs help address challenges such demonstrating in special populations, against emerging SARS-CoV-2 variants determining durability vaccine-elicited immunity. While anti-spike IgG titres and viral neutralising capacity have been characterised for contribution other components humoral immune response immediate long-term protective immunity is less well characterised. This review examines evidence supporting use trials, how they be used define It also highlights alternative biomarkers, including Fc effector function, mucosal immunity, generation long-lived plasma memory B cells discuss these applied studies tools available study them.

Language: Английский

Citations

0
Intranasal Prime–Boost with Spike Vectors Generates Antibody and T-Cell Responses at the Site of SARS-CoV-2 Infection DOI Creative Commons

Muriel Metko,

Jason M. Tonne,

Aleta Rios

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(10), P. 1191 - 1191

Published: Oct. 18, 2024

Background: Long-lived, re-activatable immunity to SARS-CoV-2 and its emerging variants will rely on T cells recognizing conserved regions of viral proteins across strains. Heterologous prime–boost regimens can elicit elevated levels circulating CD8+ that provide a reservoir first responders upon infection. Although most vaccines are currently delivered intramuscularly (IM), the initial site infection is nasal cavity. Methods: Here, we tested hypothesis heterologous prime boost vaccine regimen intranasally (IN) generate improved immune responses locally at virus compared intramuscular vaccine/booster regimens. Results: In transgenic human ACE2 murine model, both Spike-expressing single-cycle adenovirus (SC-Ad) an IFNß safety-enhanced replication-competent Vesicular Stomatitis Virus (VSV) platform generated anti-Spike antibody T-cell diminished with age. SC-Ad-Spike boosted VSV-Spike-mIFNß, alone induced maximal IgG, IgA, responses. Conclusions: There were significant differences in spleens lungs, intranasal was significantly superior generating sentinel effectors encounter lungs. These data show serious consideration should be given boosting anti-SARS-CoV-2 vaccines.

Language: Английский

Citations

0
Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study Results DOI Creative Commons
Lespek Kutumbetov, Balzhan Myrzakhmetova, Aiganym A. Tussipova

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(12), P. 1401 - 1401

Published: Dec. 12, 2024

The research conducted in this preclinical study assesses QazCovid-live, a live attenuated COVID-19 vaccine created Kazakhstan, by conducting evaluations of safety, immunogenicity, and allergenicity various animal models, including mice, rats, hamsters, guinea pigs. vaccine, developed attenuating SARS-CoV-2 via numerous Vero cell passages, had no significant adverse effects acute subacute toxicity assessments, even at elevated dosages. Allergenicity testing indicated the absence both immediate delayed hypersensitivity reactions. Immunogenicity revealed strong virus-neutralizing antibody responses, especially following intranasal intratracheal delivery. Studies on reversibility transmission further validated vaccine’s stability non-pathogenicity. data indicate that QazCovid-live is safe, immunogenic, prepared for clinical trials, presenting potential strategy prevention.

Language: Английский

Citations

0