Air sampling and ATP bioluminescence for quantitative detection of airborne microbes

Talanta, Journal Year: 2024, Volume and Issue: 274, P. 126025 - 126025

Published: March 31, 2024

Language: Английский

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Integrated mRNA and miRNA Omics Analyses Reveal Transcriptional Regulation of the Tolerance Traits by Aquatica leii in Response to High Temperature

Insects, Journal Year: 2025, Volume and Issue: 16(3), P. 316 - 316

Published: March 18, 2025

Within the context of global warming, understanding molecular mechanisms behind physiological plasticity and local adaptation is essential for insect populations. This study performed an integrated miRNA mRNA analysis on Aquatica leii larvae exposed to temperatures 20 °C, 24 28 32 °C. Under varying thermal conditions, 1983 genes exhibited differential expression (i.e., DEGs). These showed significant enrichment in Kyoto Encyclopedia Genes Genomes (KEGG) pathways related carbohydrate metabolism, glycan biosynthesis metabolism. Notably, we detected that “neuroactive ligand–receptor interaction” signaling pathway, which involved environmental information processing, was significantly upregulated °C treatment groups. indicates starting at A. needs maintain normal cellular functions by regulating binding signal transduction. Furthermore, 220 differentially expressed miRNAs (DEMs) were under different temperature conditions. An interaction network constructed between key DEMs DEGs, revealing 12 DEM-DEG regulatory pairs treatments. We found three miRNA-mRNA candidate modules could be leii’s response high temperature, including ggo-miR-1260b ptr-miR-1260b/RN001_010114, CM069438.1_43851/RN001_014852, CM069438.1_43851/RN001_014877. Our data provide deeper insights into responses levels.

Language: Английский

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NKX2‐5/LHX1 and UHRF1 Establishing a Positive Feedback Regulatory Circuitry Drives Esophageal Squamous Cell Carcinoma through Epigenetic Dysregulation

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

DNA methylation regulators play critical roles in modulating oncogenic driver genes cancers. However, the precise mechanisms through which these influence oncogenesis and clinical therapy have yet to be fully elucidated. This study reveals that hypermethylation of under-methylated regions (UMRs) within gene bodies is involved activation homeobox genes, particularly NKX2-5 LHX1, esophageal squamous cell carcinoma (ESCC). Mechanistically, LHX1 synergistically bind promoter region UHRF1, thereby augmenting its transcription. In turn, UHRF1 orchestrates recruitment DNMT1/DNMT3A, alongside UMRs increasing levels their expression. intricate interplay forms a positive transcriptional feedback loop between NKX2-5/LHX1 thus promoting overexpression all three ultimately facilitating tumor growth. Notably, concurrent inhibition DNMTs impedes growth by suppressing Overall, this identifies regulatory circuitry underlying UMR hypermethylation-mediated drivers ESCC proposes promising therapeutic strategy for patients.

Language: Английский

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Advances in arthropod-inspired bionic materials for wound healing

Materials Today Bio, Journal Year: 2024, Volume and Issue: 29, P. 101307 - 101307

Published: Oct. 22, 2024

Language: Английский

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Transcriptome and Expression Analysis of Glycerol Biosynthesis-Related Genes in Glenea cantor Fabricius (Cerambycidae: Lamiinae)

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11834 - 11834

Published: Nov. 4, 2024

Fabricius (Cerambycidae: Lamiinae) is an important pest that damages kapok trees in Southeast Asia with a wide adaptability to temperature. Glycerol protectant and energy source for insects low-temperature environments. However, glycerol biosynthesis-related genes at the molecular level are limited

Language: Английский

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