bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: April 22, 2024
ICP1, a lytic bacteriophage of
Language: Английский
Current Biology, Journal Year: 2025, Volume and Issue: 35(1), P. R32 - R44
Published: Jan. 1, 2025
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
Abstract Bacteria harness diverse defense systems that protect against phage predation 1 , many of which are encoded on horizontally transmitted mobile genetic elements (MGEs) 2 . In turn, phages evolve counter-defenses 3 driving a dynamic arms race remains underexplored in human disease contexts. For the diarrheal pathogen Vibrio cholerae higher burden its lytic phage, ICP1, patient stool correlates with reduced severity 4 However, direct molecular evidence phage-driven selection epidemic V. has not been demonstrated. Here, through clinical surveillance cholera-endemic Bangladesh, we capture acquisition parasitic anti-phage MGE, PLE11, initiated selective sweep coinciding largest cholera outbreak recent records. PLE11 exhibited potent activity co-circulating explaining rapid and dominating emergence. We identify PLE11-encoded Rta as novel responsible provide restricts tail assembly. Using experimental evolution, predict counteradaptations document eventual emergence ICP1 achieves convergent evolutionary outcome. By probing how PLEs hijack structural proteins to drive their horizontal transmission while simultaneously restricting assembly, discover manipulate assembly construct chimeric tails comprised MGE phage-encoded proteins. Collectively, our findings reveal basis natural globally significant virus clinically relevant context.
Language: Английский
Citations
1
FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: 100(5)
Published: April 10, 2024
Abstract While the One Health framework has emphasized importance of soil microbiomes for plant and human health, one most diverse abundant groups—bacterial viruses, i.e. phages—has been mostly neglected. This perspective reviews significance phages health in rhizosphere explores their ecological evolutionary impacts on ecosystems. We first summarize our current understanding diversity roles terms nutrient cycling, top-down density regulation, pathogen suppression. then consider how drive bacterial evolution soils by promoting horizontal gene transfer, encoding auxiliary metabolic genes that increase host fitness, selecting phage-resistant mutants with altered ecology due to trade-offs competitiveness virulence. Finally, we challenges avenues phage research ecosystems elucidate microbial ecosystem functioning future. conclude similar bacteria, likely play important connecting different compartments, affecting microbiome functions soils. From applied perspective, could offer novel approaches modulate optimize microbe–plant interactions enhance health.
Language: Английский
Citations
8
Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(6), P. 804 - 819
Published: June 1, 2024
Language: Английский
Citations
8
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Aug. 30, 2024
Many bacterial immune systems recognize phage structural components to activate antiviral responses, without inhibiting the function of component. These can be encoded in specific chromosomal loci, known as defense islands, and mobile genetic elements such prophages phage-inducible islands (PICIs). Here, we identify a family systems, named Tai (for 'tail assembly inhibition'), that is prevalent PICIs, P4-like satellites. protect their host population from other phages by blocking tail step, leading release tailless incapable infecting new hosts. To prevent autoimmunity, some Tai-positive have an associated counter-defense mechanism expressed during lytic cycle allows for formation. Interestingly, genes are organized non-contiguous operon, enabling coordinated expression.
Language: Английский
Citations
5
Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(12), P. 3268 - 3283
Published: Oct. 23, 2024
Language: Английский
Citations
5
mSystems, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 14, 2025
ABSTRACT The continuous interaction between phages and their respective hosts has resulted in the evolution of multiple bacterial immune mechanisms. However, diversity prevalence antiviral defense systems complex communities are still unknown. We therefore investigated abundance viral 3,038 high-quality archaeal genomes from rumen. In total, 14,241 31,948 antiviral-related genes were identified. Those represented 114 unique system types grouped into 49 families. observed a high genomes. number systems, families, density varied widely genome to genome. Additionally, per correlated positively with families size. Restriction modification, Abi, cas most common, but many rare present only 1% Antiviral prevalent diverse rumen, few dominant, indicating that rarely present. collection throughout rumen may represent pool mechanisms can be shared by different members community modulate phage–host interaction. IMPORTANCE Phages act antagonistically at cell level have mutualistic microbiome level. This shapes structure microbial is mainly driven mechanism. such mechanism larger than previously thought. Because that, we described genomes, metagenome-assembled (MAGs) isolates, While seem among bacteria archaea, common. suggests these not microbes could community. consistent “pan-immune system” model, which appears common across environments.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 27, 2025
Summary A unique feature of temperate phages is the ability to protect their host bacteria from a second phage infection. Such protection granted at lysogenic state, where persist as prophages integrated within bacterial chromosome, expressing genes that defend and themselves predation. Here, we report prophage-encoded anti-phage defense system inhibits DNA packaging invading in Listeria monocytogenes . This includes protein, TerI, two self-immunity proteins, anti-TerI1 anti-TerI2. TerI targets terminase complex prevent translocation into procapsids without halting lytic cycle, leading release unpacked non-infectious upon lysis. In contrast, anti-TerI2, counteract during prophage induction allow virion production. system, TERi, prevalent phages, providing population-level compromising lifecycle.
Language: Английский
Citations
0
Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13
Published: Feb. 24, 2025
Serine integrases (Ints) have gained prominence and been extensively used in Synthetic Biology due to their ability modify DNA sequences. Ints are recombinases encoded by the phage genome unidirectionally catalyze an insertion, excision, or inversion of a specific sequence between two attachment sites (att) attB (bacterial site) attP (phage site). The entire process is highly accurate; therefore, widely genetic engineering studied unique site-specific recombination properties potential editing applications. Furthermore, new recombinational factors (RDFs) determinants constantly being discovered, underlining need update progress research involving eukaryotic cells. In this way, review aims provide overview cells highlight how can be innovative ways advance applications health, agriculture, environmental sciences.
Language: Английский
Citations
0
Discover Life, Journal Year: 2025, Volume and Issue: 55(1)
Published: April 3, 2025
Language: Английский
Citations
0