Comparative anatomy of siphophage tails before and after interaction with their receptor

Current Opinion in Structural Biology, Journal Year: 2025, Volume and Issue: 92, P. 103045 - 103045

Published: April 24, 2025

Language: Английский

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A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 52(17), P. 10431 - 10446

Published: Aug. 16, 2024

Abstract ICP1, a lytic bacteriophage of Vibrio cholerae, is parasitized by phage satellites, PLEs, which hijack ICP1 proteins for their own horizontal spread. PLEs' dependence on ICP1’s DNA replication machinery and virion components results in inhibition lifecycle. PLEs are expected to depend factors genome packaging, but the mechanism(s) use inhibit packaging currently unknown. Here, we identify characterize Gpi, PLE’s indiscriminate inhibitor. Gpi binds large terminase (TerL), motor, blocks packaging. To overcome Gpi's negative effect TerL, component PLE also requires, uses two specifiers, GpsA GpsB, that specifically allow genomes. Surprisingly, mimicry pac site as backup strategy ensure mimicry, however, only sufficient if can at other stages its lifecycle, suggesting an advantage maintaining GpsB. Collectively, these provide mechanistic insights into another stage lifecycle inhibited PLE, most inhibitory documented satellites. More broadly, represents first satellite-encoded inhibitor TerL.

Language: Английский

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GMT systems define a new class of mobile elements rich in bacterial defensive and offensive tools

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 28, 2023

Abstract Conflicts between bacteria and their rivals led to an evolutionary arms race the development of bacterial immune systems. Although diverse immunity mechanisms were recently identified, many remain unknown, dissemination within is poorly understood. Here, we describe a widespread genetic element, defined by presence Gamma-Mobile-Trio (GMT) proteins, that serves as survival kit. We show GMT-containing genomic islands are active mobile elements with cargo comprising various anti-phage defense systems, in addition antibacterial type VI secretion system (T6SS) effectors antibiotic resistance genes. identify four new systems encoded GMT islands. A thorough investigation one reveals it triggered phage capsid protein induce cell dormancy. Our findings underscore need broaden concept ‘defense islands’ include also offensive tools, such T6SS effectors, they share same defensive tools for dissemination.

Language: Английский

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Prophages in Vibrio

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Although tailed bacteriophages (phages) of the class Caudoviricetes are thought to constitute most abundant and ecologically relevant group phages that can integrate their genome into host chromosome, it is becoming increasingly clear other prophages widespread. Here, we show derived from filamentous tailless with sizes below 16 kb make up majority in marine bacteria genus Vibrio. To estimate prophage prevalence unaffected by database biases, combined comparative genomics chemical induction 58 diverse Vibrio cyclitrophicus isolates, resulting 107 well-curated prophages. Complemented computationally predicted prophages, obtained 1158 931 naturally co-existing strains family Vibrionaceae. Prophages resembling predominated, accounting for 80% all V. 60% across In our experimental model, three viral realms actively replicated upon indicating ability transfer new hosts. Indeed, were rapidly gained lost, as suggested variable content between closely related cyclitrophicus. integrated only genomic locations restored function integration site. Despite small size, they contained highly accessory genes may contribute fitness, such phage defense systems. We propose that, like well-studied equivalent, temperate active drivers ecology evolution Vibrio, which have been largely overlooked.

Language: Английский

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A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 22, 2024

ICP1, a lytic bacteriophage of

Language: Английский

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