Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 3, 2024
Language: Английский
Small Structures, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 19, 2025
To effectively address crisis emergence of new virus such as monkeypox, a collective and collaborative effort between scientists, engineers, innovators, artists from all ages, regions, diverse fields is required. This review explores holistic approach to addressing the monkeypox by integrating nanobiosensors, artificial intelligence, visual arts, humanities, social sciences. Traditional diagnostic methods are often limited time, accessibility, accuracy, but advancement point‐of‐care smart nanobiosensors offers promising shift toward rapid, precise, accessible diagnostics. They enhance ability screen, diagnose, monitor infections efficiently, contributing better disease management. Beyond technological innovation, essential role sciences in fostering public engagement, understanding, acceptance tools emphasized. Visual arts can illustrate scientific concepts, making them more relatable, while storytelling through various media reduce stigma promote preventive measures. Social provide insights into cultural attitudes, behaviors, health challenges, ensuring that solutions integrated communities. By combining these disciplines, this presents comprehensive framework for resilient global system aligns with One Health principles, emphasizing interconnectedness human, animal, environmental health.
Language: Английский
Citations
1
Viruses, Journal Year: 2024, Volume and Issue: 16(5), P. 753 - 753
Published: May 10, 2024
Avian influenza viruses (AIVs) of the H5 subtype rank among most serious pathogens, leading to significant economic losses in global poultry industry and posing risks human health. Therefore, rapid accurate virus detection is crucial for prevention control AIVs. In this study, we established a novel method by utilizing precision CRISPR/Cas12a efficiency RT-RPA technologies. This assay facilitates direct visualization results through blue light lateral flow strips, accurately identifying with high specificity without cross-reactivity against other AIV subtypes, NDV, IBV, IBDV. With thresholds 1.9 copies/μL (blue light) × 103 (lateral strips), our not only competes but also slightly surpasses RT-qPCR, demonstrating an 80.70% positive rate across 81 clinical samples. The RT-RPA/CRISPR-based characterized sensitivity, specificity, independence from specialized equipment. immediate field applicability RT-RPA/CRISPR approach underscores its importance as effective tool early management outbreaks caused
Language: Английский
Citations
7
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 154174 - 154174
Published: July 21, 2024
Language: Английский
Citations
7
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 269, P. 116932 - 116932
Published: Nov. 14, 2024
Diagnostic approaches that combine the high sensitivity and specificity of laboratory-based digital detection with ease use affordability point-of-care (POC) technologies could revolutionize disease diagnostics. This is especially true in infectious diagnostics, where rapid accurate pathogen critical to curbing spread disease. We have pioneered an innovative label-free platform utilizes Interferometric Reflectance Imaging Sensor (IRIS) technology. IRIS leverages light interference from optically transparent thin film, eliminating need for complex optical resonances enhance signal by harnessing power averaging shot-noise-limited operation In our latest work, we further improved previous 'Single-Particle' (SP-IRIS) technology allowing construction signature target nanoparticles (whole virus) a single image. new platform, 'Pixel-Diversity' (PD-IRIS), eliminated z-scan acquisition, required SP-IRIS, time-consuming expensive process, made more applicable POC settings. Using PD-IRIS, quantitatively detected Monkeypox virus (MPXV), etiological agent (Mpox) infection. MPXV was captured anti-A29 monoclonal antibody (mAb 69-126-3) on Protein G spots sensor chips were at limit-of-detection (LOD) - 200 PFU/mL (∼3.3 aM). PD-IRIS superior ELISA (LOD 1800 PFU/mL) used as comparator. The demonstrated using Herpes simplex virus, type 1 (HSV-1), Cowpox (CPXV). work establishes effectiveness opens possibilities its advancement clinical diagnostics Mpox POC. Moreover, modular can be adapted multiplex pathogens which high-affinity ligands are available bind their surface antigens capture them surface.
Language: Английский
Citations
5
Biosafety and Health, Journal Year: 2024, Volume and Issue: 6(5), P. 260 - 269
Published: Sept. 11, 2024
Language: Английский
Citations
4
Bioengineering & Translational Medicine, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 2, 2025
Abstract Human Mpox disease (MPX) is an endemic zoonotic that develops when patients are infected with the virus (MPXV). MPXV shares a high level of genetic similarity to other poxviruses and clinical presentation MPX similar poxvirus infections which can result in delay diagnosis. In addition, phylogenetically divided into two different clades affects severity disease. recent years, there has been unusual worldwide spread MPXV, leading global public health problem. The most important step fight against rapid, highly specific, accurate Following rapid efforts develop diagnostic tests have gained momentum. Here, MPX, epidemiology, discussed. Furthermore, biochemical tests, molecular their development, point‐of‐care (PoC) applications reviewed. Molecular technologies such as polymerase chain reaction, recombinase amplification, loop‐mediated isothermal amplification methods detect evaluated. Additionally, next‐generation combined techniques importance PoC transition explored.
Language: Английский
Citations
0
Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2025, Volume and Issue: unknown, P. 245 - 275
Published: Jan. 1, 2025
Language: Английский
Citations
0
Small, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 13, 2025
Abstract Point‐of‐care (POC) pathogen detection is highly desirable in diverse fields such as infectious disease diagnosis, food safety testing, and environmental monitoring. Herein, the study seeks to address this critical need by developing an automated microfluidic photothermal quantitative polymerase chain reaction (AMP‐qPCR) system a greatly simplified format. A key element of AMP‐qPCR architecture that combines design clockwork‐like, magnetically‐driven multi‐chamber cartridge with use cheap black tape beneath PCR chamber fast photothermal‐responsive engine. This not only enables unprocessed sample be lysed, purified, subjected real‐time fluorescence ultracompact autonomous manner but also eliminates for sophisticated photonic material/device fabrication frequently required performing ultrafast PCR. It shown can accomplish high‐efficient bacterial DNA extraction within 18.5 min, enabling accurate quantification bacteria concentration from 10 8 2 CFU·mL −1 . Furthermore, its practical applicability demonstrated detecting Neisseria gonorrhoeae sexually transmitted infection‐suspected patients using clinical urine cervical swab specimens, exhibiting matched performance benchtop machine. The presented platform enhances availability POC molecular diagnostics on‐site in‐home testing.
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 18, 2025
The recent monkeypox epidemic outbreaks worldwide highlight the urgent need for fast and precise diagnostic solutions, especially in resource-limited settings. Here, a two-dimensional nanozyme-catalyzed colorimetric CRISPR assay microfluidic detection of virus (MPXV) was established. We utilized graphene oxide as substrate adsorption gold seeds deposition porous Pt shell to prepare high-performance GO@Pt nanomaterials. viral nucleic acids released from clinical samples initiated single-step recombinase polymerase amplification-CRISPR/Cas13a trans-cleavage ssRNA reporters labeled with FAM biotin. These can be recognized by antibody-conjugated nanozymes streptavidin-coated magnetic beads. formed sandwich immunocomplexes catalyze oxidation colorless 3,3′,5,5′-tetramethylbenzidine distinct color change. proposed GO@Pt-catalyzed exhibited limit 1 copy/μL MPXV 60 min. Forty samples, including rash fluid swabs oral swabs, were tested 100% agreement real-time PCR. results indicate excellent potential sensitive accurate testing under resource-constrained conditions.
Language: Английский
Citations
0
ACS Sensors, Journal Year: 2025, Volume and Issue: 10(2), P. 575 - 576
Published: Feb. 28, 2025
InfoMetricsFiguresRef. ACS SensorsVol 10/Issue 2Article This publication is free to access through this site. Learn More CiteCitationCitation and abstractCitation referencesMore citation options ShareShare onFacebookX (Twitter)WeChatLinkedInRedditEmailJump toExpandCollapse EditorialFebruary 28, 2025Advancing CRISPR/Cas Biosensing with Integrated DevicesClick copy article linkArticle link copied!Guozhen Liu*Guozhen LiuIntegrated Devices Intelligent Diagnosis (ID2) Laboratory, School of Medicine, The Chinese University Hong Kong, Shenzhen 518172, China*Email: [email protected]More by Guozhen Liuhttps://orcid.org/0000-0002-0556-6404Open PDFACS SensorsCite this: Sens. 2025, 10, 2, 575–576Click citationCitation copied!https://pubs.acs.org/doi/10.1021/acssensors.5c00330https://doi.org/10.1021/acssensors.5c00330Published February 2025 Publication History Received 27 January 2025Published online 28 in issue 2025editorialCopyright © American Chemical Society. available under these Terms Use. Request reuse permissionsThis licensed for personal use PublicationsCopyright SocietySubjectswhat are subjectsArticle subjects automatically applied from the Subject Taxonomy describe scientific concepts themes article.AssaysBiosensingBiotechnologyGeneticsSensorsRather than being famous only gene editing field, revealing collateral cleavage activity Cas12a, Cas13a, Cas14 effectors, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas) systems (i.e., CRISPR/Cas) have received significant credit modern analytical science capability detecting versatile analytes superior sensitivity specificity. (1,2) A variety exciting biosensing now been developed successfully detection different varying nucleic acids non-nucleic (such as metabolites, proteins, exosomes, metal ions). Although most popular signal output biosensors fluorescence, various modalities such colorimetric, electrochemiluminescence, electrochemical, electrical systems. Furthermore, potential has demonstrated multiplex integration microfluidics or other devices enabling identification presence multiple targets. However, despite extensive efforts success develop diagnostic tools based on trans-cleavage enzymatic activity, encounter unavoidable challenges, including inadequate limit (near picomole level) clinically relevant biomarkers at subpicomolar levels limited catalytic efficiency DNA cleavage. These limitations significantly hinder widespread adoption clinical diagnostics point-of-care testing.To further enhance avoid necessity sophisticated costly equipment, acids-based preamplification techniques, thermal-dependent amplification, polymerase chain reaction (PCR), thermal-independent rolling circle amplification (RCA), recombinase (RPA), loop-mediated isothermal (LAMP), frequently integrated assays. techniques increase sensitivity, they inevitably overshadow Cas effectors neglect intrinsic effectors. Preamplification also extends time reduces subsequent due nonspecific primer interference, while substantially increasing risk aerosol contamination. sensitive acid strategies employ exponential formats which amplicons (amplification products) recycled primers templates. because format, background products that lead false-positive results inevitable after long times can be caused by, example, contaminants, off-template products, secondary structures Therefore, evaluated controlled practice generation unwanted signals. Consequently, amplification-based always determined resolution between true- signals generated diluted standard samples blank/negative controls, respectively.Development preamplification-free aims achieve rapid one-pot high (attomolar single-molecule levels) adaptability. achieved optimizing key components reporters), employing cascade adopting microfluidic droplet analysis, integrating readout patterns. Conventional single-strand reporters replaced a range nanoparticle-based reporters, (3) gold nanoparticle (AuNP) quantum dot platinum nanoparticles, aggregation-induced emission agent reporters. AuNPs were used CRISPR/Cas12a biosensor surface enhanced Raman spectroscopy (SERS) low 10 aM. (4) By utilizing autocatalytic circuits, enabled attomolar without need preamplification. (5) ability detect acids, immunoassays into system abundance. (6)CRISPR/Cas technologies promise beyond simple assay. (7) CRISPR technology engineering led advances molecular biology healthcare vitro diagnosis vivo monitoring, tube assays devices. (8−10) lateral flow assay, face mask incorporated lyophilized sensor was noninvasive SARS-CoV-2 room temperature 90 min requires no user intervention press button. (11) Microfluidic paper-based realize supersensitive pathogenic bacteria foods. (10) wearable microneedle patch uses CRISPR-activated graphene biointerfaces reported extraction long-term monitoring universal cell-free DNA. It enables real-time over days vivo, highlighting its early disease screening prognosis. (12) single-step monkeypox virus 15 vest-pocket device 0.5 copies μL–1 100% concordance PCR validation, (13) adaptable resource-limited settings. Multiplex challenging certain enzymes, resulting interference possible cross-reactivity among analytes. (14) Solutions, high-fidelity variants, coupling optimal crRNA designs, using orthogonal technologies, allow simultaneous targets interference. We recently CRISPR-Cas12a immunosensing glass fiber portable fluorescence reader proteins pM limit, cytokines synovial joint fluids scenario. (6) eliminating expensive instrument tedious sample preparation, CRISPR/Cas-mediated provide sample-to-answer will continue bring about breakthrough diagnosis. (10,11)CRISPR/Cas-based much away maturation. editorial encourages submissions demonstrate advancements existing address growing quick, cost-effective, sensitive, accurate field-deployable vivo. With nanotechnologies, microfluidics, enzyme-based systems, artificial intelligence, our aim facilitate comprehensive reliable analyte detection. (15) And it throughput biomarker discovery (16) designing "all-in-one" combine processing, readout. Real-time target bioimaging another highlight research biomedical field if novel discovered integrate continuous corresponding typically involve steps, extraction, readout, become time-consuming prone In addition integration, respond innovations enzyme reagent assay desirable reduced time. Realizing full platforms sustained interdisciplinary collaboration scientists, biologists, engineers, clinicians, policy makers. Continued innovation components, regulatory standardization essential translate cutting-edge laboratory bedside beyond, although there challenges. confident match proudly not but affordable globally accessible, driving improvements precision medicine sustainability science.Author InformationClick section linkSection copied!Corresponding AuthorGuozhen Liu, China, https://orcid.org/0000-0002-0556-6404, Email: protected]NotesViews expressed those author necessarily views ACS.ReferencesClick copied! references 16 publications. 1Chertow, D. S. Next-generation CRISPR. Science 2018, 360, 381– 382, DOI: 10.1126/science.aat4982 Google Scholar1Next-generation CRISPRChertow Daniel S.Science (Washington, DC, United States) (2018), 360 (6387), 381-382CODEN: SCIEAS; ISSN:0036-8075. (American Association Advancement Science) There expanded reference. >> SciFinder ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1cXpt1Witbs%253D&md5=b83cc13b01ff95f5b9dba8b21ee480c02Li, Y.; Li, S.; Wang, J.; G. towards next-generation biosensing. Trends Biotechnol. 2019, 37, 730– 743, 10.1016/j.tibtech.2018.12.005 Scholar2CRISPR/Cas Systems Next-Generation BiosensingLi, Yi; Shiyuan; Jin; GuozhenTrends Biotechnology (2019), 37 (7), 730-743CODEN: TRBIDM; ISSN:0167-7799. (Elsevier Ltd.) review. Beyond remarkable genome ability, CRISPR/Cas9 effector utilized applications. recent RNA Cas13a sparked even greater interest developing promised diagnostics. Now, along Cas12 activities single-stranded (ssDNA), several established targets, bacteria, viruses, cancer mutations, others. Based we detailed classification propose their future utility. As continues mature, promising candidates platforms. ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1MXksVyntQ%253D%253D&md5=99d745d312b053b1c9582bcbd832ab923Ki, J. CRISPR/Cas-assisted colorimetric point-of-use testing African swine fever virus. 2022, 7, 3940– 3946, 10.1021/acssensors.2c02007 Scholar3CRISPR/Cas-Assisted Colorimetric Biosensor Point-of-Use Testing Swine Fever VirusKi, Jisun; Na, Hee-Kyung; Yoon, Sun Woo; Le, Van Phan; Lee, Tae Geol; Lim, Eun-KyungACS Sensors (2022), 7 (12), 3940-3946CODEN: ASCEFJ; ISSN:2379-3694. Society) (ASFV) causes highly contagious fatal affecting both domesticated wild pigs. Substandard therapies vaccinations cause severe economic damages pig culling removal infected carcasses. an urgent approach assists avoiding spread ASFV reducing loss. study, sensing platform dual enzymic combined clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-assocd. protein 12a (Cas12a) urease ASFV. mechanism involves magnetic bead-anchored urease-conjugated oligodeoxynucleotide (MB@urODN), dsDNA cleaved activated CRISPR/Cas12a. After magnetically sepg. urease, confirmed measuring change soln. advantage method undergoing complex duplication process. detected three clin. specimens collected porcine tissue samples. proposed designed adequate, simple, robust, selective anal. technique zoonotic vast specialized tools. ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XivFSgsrbJ&md5=192b3a94a2eeda9308c22970296517d24Yin, B. SERS nanoplatform chimeric DNA/RNA hairpin guide ultrasensitive Theranostics 12, 5914, 10.7150/thno.75816 Scholar4A detectionYin, Bohan; Zhang, Qin; Xia, Xinyue; Chuanqi; Ho, Willis Kwun Hei; Yan, Jiaxiang; Huang, Yingying; Wu, Honglian; Pui; Yi, Changqing; Hao, Jianhua; Jianfang; Chen, Honglin; Wong, Siu Dexter; Yang, MoTheranostics 12 (13), 5914-5930CODEN: THERDS; ISSN:1838-7640. (Ivyspring International Publisher) nanomaterial-based optical surface-enhanced scattering (SERS), formulate powerful amplification-free system. nanomaterials impose steric hindrance accessibility narrow gaps (SERS hot spots) nanoparticles (NPs) producing To overcome restriction, specifically design hairpins (displacers) destabilized DNA, liberating excessive disintegrate core-satellite nanocluster via toehold-mediated strand displacement orchestrating "on-off" biosensor. comprises large core surrounded small tags hybridization ultrabright reporter, disassembly leads drastic decrease intensity readouts. introduce sepn. disassembled nanostructures suppress improving sensitivity. proof-of-concept findings showed application displacers more effective decreasing attained better (LOD, aM) directly CRISPR-Cas12a, selectivity stability Introducing magnetic-responsive functionality improves LOD 1 Our work offers sensitively selectively probe pre-amplification provides new insights CRISPR-Cas12a/SERS resolve constructing biosensors. ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38Xit12nurzK&md5=33e4d73cf233e629bd2c56718062532c5Shi, K. CRISPR-Cas autocatalysis-driven feedback network Sci. Adv. 2021, eabc7802 10.1126/sciadv.abc7802 ScholarThere record reference.6Zou, quantification inflammation osteoarthritis. Device 2024, 100319, 10.1016/j.device.2024.100319 reference.7Shi, R.; Zhong, L.; G.; Mak, W. C. Technology: From Lab Assays Portable Wearables. TrAC Anal. Chem. 177, 117796, 10.1016/j.trac.2024.117796 reference.8Broughton, P. CRISPR–Cas12-based SARS-CoV-2. Nat. 2020, 38, 870– 874, 10.1038/s41587-020-0513-4 Scholar8CRISPR-Cas12-based SARS-CoV-2Broughton, James P.; Deng, Xianding; Yu, Guixia; Fasching, Clare Servellita, Venice; Singh, Jasmeet; Miao, Xin; Streithorst, Jessica A.; Granados, Andrea; Sotomayor-Gonzalez, Alicia; Zorn, Kelsey; Gopez, Allan; Hsu, Elaine; Gu, Wei; Miller, Steve; Pan, Chao-Yang; Guevara, Hugo; Wadford, Debra Janice Chiu, Charles Y.Nature (2020), 38 870-874CODEN: NABIF9; ISSN:1087-0156. (Nature Research) Abstr.: An outbreak betacoronavirus acute respiratory syndrome (SARS)-CoV-2 began Wuhan, China Dec. 2019. COVID-19, assocd. infection, rapidly produce global pandemic. report development (<40 min), easy-to-implement CRISPR-Cas12-based swab exts. validated contrived ref. patients States, 36 COVID-19 infection 42 viral infections. CRISPR-based DETECTR visual faster alternative US Centers Disease Control Prevention RT-PCR 95% pos. predictive agreement neg. agreement. ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXntlejt7w%253D&md5=a1ccd699e2945474307f349518da37e59Lee, I.; Kwon, S.-J.; Heeger, Dordick, Ultrasensitive ImmunoMag-CRISPR Lateral Flow Assay Point-of-Care Urinary Biomarkers. 9, 92– 100, 10.1021/acssensors.3c01694 reference.10Nguyen, Q. Wearable materials embedded synthetic sensors biomolecule 39, 1366– 1374, 10.1038/s41587-021-00950-3 Scholar10Wearable detectionNguyen, Peter Q.; Soenksen, Luis Donghia, Nina M.; Angenent-Mari, Nicolaas de Puig, Helena; Ally; Rose; Slomovic, Shimyn; Galbersanini, Tommaso; Lansberry, Geoffrey; Sallum, Hani Zhao, Evan Niemi, B.; Collins, J.Nature (2021), 39 (11), 1366-1374CODEN: Portfolio) Integrating biol. wearables could expand opportunities physiol. status, states exposure pathogens toxins. operation circuits generally living, engineered wearables. Here lightwt., flexible substrates textiles functionalized freeze-dried, tools, chems. pathogen signatures. upon rehydration aq. events specific mol. changes detects fluorescent luminescent outputs. limits rival current lab. methods quant. PCR. wearable, temp. within min, requiring ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXhsVenurnK&md5=70c78eaf02d067096407bd97d4ceb1ea11Dai, Gooding, mediated toward understanding cellular Angew. Chem., Int. Ed. 59, 20754– 20766, 10.1002/anie.202005398 Scholar11CRISPR Mediated Toward Understanding Cellular Biology DiagnosisDai, Yifan; Yanfang; Guozhen; JustinAngewandte Chemie, Edition 59 (47), 20754-20766CODEN: ACIEF5; ISSN:1433-7851. (Wiley-VCH Verlag GmbH & Co. KGaA) Recent biotechnologies greatly authors' capabilities repurpose biomol. diagnosing diseases pathways. attribute allows widely programmable mechanism. Minireview, authors first illustrate principle functioning process actuating. Next, mols. summarized. some applications biomols. imaging networks. Finally, challenges with, prospects of, developments discussed. ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXhs1Oks7nP&md5=0119652222aa5d39b8f64ccb8aceb96f12Yang, Fang, X. Programmable CRISPR-Cas9 capture Commun. 13, 3999, 10.1038/s41467-022-31740-3 Scholar12Programmable DNAYang, Bin; Jilie; XueenNature Communications 13 (1), 3999CODEN: NCAOBW; ISSN:2041-1723. health. modules, microneedles ext. interstitial fluid minimally invasive fashion. perform extn. macromol. simultaneously. show synergetic effect biointerfaces, Epstein-Barr virus, sepsis, kidney transplantation anti-interference 60% fetal bovine serum, satisfactory stable exptl. immunodeficient mouse models shows feasibility practicability method. holds great potentially ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XhvVChtr3N&md5=1e4c4fdfe726801050f38a8fda03c78d13Wang, Y. minutes device. 15, 3279, 10.1038/s41467-024-47518-8 reference.14Li, multiplexed biosensing: challenge insurmountable obstacle?. 792– 795, 10.1016/j.tibtech.2019.04.012 Scholar14CRISPR/Cas Multiplexed Biosensing: Challenge Insurmountable Obstacle?Li, Linyang; (8), 792-795CODEN: Performing still elusive goal CRISPR/Cas-based Instead obstacle, realistic successful Strategic considerations required fully explore ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1MXpsVOrsL4%253D&md5=414b5e06e7ed5c55a528e930054e0b8815Song, Amplifying mutational profiling extracellular vesicle mRNA SCOPE. 10.1038/s41587-024-02426-6 reference.16Rahimi, Balusamy, Perumalsamy, H.; Ståhlberg, Mijakovic, I. recognition biomarkers. Nucleic Acids Res. 52, 10040– 10067, 10.1093/nar/gkae736 reference.Cited Click copied!This yet cited publications.Download PDFFiguresReferences Get e-AlertsGet e-AlertsACS copied!https://doi.org/10.1021/acssensors.5c00330Published 2025Copyright permissionsArticle Views-Altmetric-Citations-Learn metrics closeArticle Views COUNTER-compliant sum text downloads since November 2008 (both PDF HTML) across all institutions individuals. updated reflect usage leading up last few days.Citations number articles citing article, calculated Crossref daily. Find information counts.The Altmetric Attention Score quantitative measure attention online. Clicking donut icon load page altmetric.com additional details score social media given article. how calculated.Recommended Articles FiguresReferencesThis figures.References 1Next-generation 2CRISPR/Cas 3CRISPR/Cas-Assisted 4A 8CRISPR-Cas12-based 10Wearable 11CRISPR 12Programmable 14CRISPR/Cas
Language: Английский
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