Metagenomic Analysis Revealed the Changes in Antibiotic Resistance Genes and Heavy Metal Resistance Genes in Phosphate Tailings Compost DOI Creative Commons
Chunqiao Xiao,

Kai Wan,

Yan Chen

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 768 - 768

Published: March 28, 2025

Phosphate tailings are usually rich in phosphorus and some other mineral nutrients, which is very suitable for composting. In this study, 60 days of composting using phosphate tailings, chicken manure, straw resulted a significant decrease total nitrogen (TN) content from 1.75 ± 0.12 g/kg to 0.98 0.23 (p < 0.01), with retention 56%, an increase water-soluble (Ws-P) 3.24 0.14 mg/kg 7.21 0.09 mg/kg, immediate potassium (AK) 0.56 0.21 1.90 0.11 0.05). Metagenomic sequencing showed little changes the diversity abundance microbial communities before after composting, but species composition archaea, bacteria, fungi differences community structure Composting contributed lower gene ARGs MRGs. The addition combined functions chemical regulation nutrient enrichment, its synergistic effect significantly optimized cycling system.

Language: Английский

Harnessing the plant microbiome for environmental sustainability: From ecological foundations to novel applications DOI

Jing-Ru Hao,

李岩 Li Yan,

Yuan Ge

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175766 - 175766

Published: Aug. 24, 2024

Language: Английский

Citations

6
The role of the rhizobiome recruited by root exudates in plant disease resistance: current status and future directions DOI Creative Commons
Zengwei Feng, Qingfeng Liang, Qing Yao

et al.

Environmental Microbiome, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 16, 2024

Language: Английский

Citations

6
Root hair developmental regulators orchestrate drought triggered microbiome changes and the interaction with beneficial Rhizobiaceae DOI Creative Commons
Zhenghong Wang, Zewen Li, Yujie Zhang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 20, 2024

Drought is one of the most serious abiotic stresses, and emerging evidence suggest plant microbiome affects drought tolerance. However, there a lack genetic regarding whether how plants orchestrate dynamic assembly upon drought. By utilizing mutants with enhanced or decreased root hair densities, we find that regulators also affect induced changes. Rhizobiaceae key biomarker taxa affected by related mutants. We isolated sequenced 1479 associated microbes, confirmed several Rhizobium strains presented stress-alleviating activities. Metagenome, transcriptome metabolome studies further reveal multi-omic changes stress. knocked out an ornithine cyclodeaminase (ocd) gene in sp. 4F10, which significantly dampens its stress alleviating ability. Our integrated multi-omics confirm involvement host effects reshaping during drought, provide mechanistic insights into mediated protection. Plant confers fitness benefits integrating genetic, microbial approaches, this work reveals critical role developmental shaping microbiome.

Language: Английский

Citations

5
Host genetic variation and specialized metabolites from wheat leaves enriches for phyllosphere Pseudomonas spp. with enriched antibiotic resistomes DOI Creative Commons
Qian Xiang, Da Lin,

Zaijun Yang

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Antibiotic resistance in plant-associated microbiomes poses significant risks for agricultural ecosystems and human health. Although accumulating evidence suggests a role plant genotypes shaping their microbiome, almost nothing is known about how the changes of genetic information affect co-evolved microbiome carrying antibiotic genes (ARGs). Here, we selected 16 wheat cultivars experimentally explored impact host variation on phyllosphere ARGs, metabolites. Our results demonstrated that significantly influenced resistomes. Wheat exhibiting high ARGs were linked to elevated Pseudomonas populations, along with increased abundances aeruginosa biofilm formation genes. Further analysis 350 spp. genomes from diverse habitats at global scale revealed nearly all strains possess multiple virulence factor (VFGs), mobile elements (MGEs) genomes, albeit lower nucleotide diversity compared other species. These findings suggested proliferation contributed resistance. We further observed direct links between upregulated leaf metabolite DIMBOA-Glc, spp., enrichment which corroborated by microcosm experiments demonstrating DIMBOA-Glc enhanced relative abundance Overall, alterations metabolites resulting throughout evolution may drive development highly specialized microbial communities capable enriching ARGs. This study enhances our understanding plants actively shape clarifies

Language: Английский

Citations

4
Analysis of the Synergistic Effect of Endophytic Bacteria and Metabolites on Yield in Soybean Roots Under Biochar with Nitrogen Fertilization DOI Creative Commons
Hui Liu,

Wanyu Dou,

Wenlong Zhang

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 177 - 177

Published: Jan. 13, 2025

The appropriate application of nitrogen fertilizers and biochar to the soil is beneficial for soybean growth, a combination can improve low-nitrogen fertilizer utilization efficiency. However, effect on roots still unclear. Therefore, in this study, we investigate changes endophytic bacterial communities metabolites under different combinations treatments by setting fertilization levels using high-throughput sequencing non-targeted metabolomics techniques. results showed that applying 20 t/ha 180 kg/ha (C2N1) resulted highest yield. By studying community roots, it was observed dominant phyla were Proteobacteria, Bacteroidota, Actinobacteriota, genera Bradyrhizobium, Streptomyces, Rhizobacter. Moreover, metabolic pathway analysis revealed glycosylphosphatidylinositol (GPI)-anchor biosynthesis most significant C0N1 vs. C0N0 comparison group, glycerophospholipid metabolism C2N1 C1N1 group; they both associated with regulatory mechanisms such as plant growth promotion nutrient cycling. Structural equation modeling indicates bacteria have positive impact Bradyrhizobium Rhizobium are observable variables effects bacteria, while Phe-Thr 7-allyloxycoumarin metabolites. Endophytic not only direct crop yield, but also indirectly affect yield through their This study has important scientific value guiding significance improving yields.

Language: Английский

Citations

0
Diversity, characterization, and biotechnological potential of plant growth-promoting bacteria from Bryophyllum pinnatum (Lam.) (Crassulaceae) roots and rhizosphere soil DOI
Jianyun Zhang, Siyu Liao,

Wei-Ping Gao

et al.

International Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Language: Английский

Citations

0
Deciphering the Dynamic Interplay between Rhizobacteria and Root Exudates via Cerium Oxide Nanomaterials Modulation for Promoting Soybean Yield and Quality DOI
Liya Jiao, Xuesong Cao, Chuanxi Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

The interplay between root exudates and rhizobacteria is essential for enhancing agricultural productivity. Herein, the impacts of cerium dioxide nanomaterials (CeO2 NMs) on these interactions in soybean plants were investigated. Following 3-5 weeks exposure to 5 mg·kg-1 CeO2 NMs, composition changed over time, with isoflavone levels increasing by 6.3-21.7 folds, potentially manipulating rhizobacteria. Correspondingly, such as Ensifer, Allorhizobium, Nitrospira, Bradyrhizobium enriched 40.7-367.3% at three time points. NMs stimulated biosynthesis their excretion into rhizosphere via upregulating expressions MYB transcription factors, biosynthesis, transporter genes. mediated enhanced plant biomass (45.5-75.9%), nodulation (85.7%), nitrogen fixation, nutrient acquisition, soil health, improving quality (34.4-223.9%) yield (16.2%). This study provides insights exudate-rhizobacteria leguminous facilitated sustainable agriculture.

Language: Английский

Citations

0
Analysis of Genetic Diversity Among Wild Soybean (Glycine soja) in Hebei Province, China DOI Creative Commons

Xintong Wu,

Feng Qiu, Zhanying Zhang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract Objectives: Soybean serves as a crucial source of protein and oil. Wild soybean (Glycine soja) shares genetic similarities with cultivated max) but exhibits richer diversity due to lower bottlenecks. The high allelic in wild soybeans provides traits for environmental adaptation, which is useful through breeding. Considering that originated northern China Hengshui Lake, wetland environment, plays role preserving species diversity, 17 resources at this site were collected then re-sequenced on the Illumina NovaSeq6000 platform depth 10×. Data description: In study, we accessions from Lake Hebei Province, China, performed re-sequencing platform, followed by SNPs identification. Subsequently, incorporated an additional 62 genomic datasets utilized ADMIXTURE, neighbor-joining tree, principal component analysis elucidate population characteristics. results revealed these could be divided into seven distinct subpopulations.

Language: Английский

Citations

0
Stable soil moisture promotes shoot performance and shapes the root-rhizosphere microbiome DOI Creative Commons

Dichuan Liu,

Zhuan Wang,

Guolong Zhu

et al.

Agricultural Water Management, Journal Year: 2025, Volume and Issue: 310, P. 109354 - 109354

Published: Feb. 11, 2025

Language: Английский

Citations

0
Plant–microbiome interactions and their impacts on plant adaptation to climate change DOI Open Access
Qing Zeng, Hang‐Wei Hu, An‐Hui Ge

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

ABSTRACT Plants have co‐evolved with a wide range of microbial communities over hundreds millions years, this has drastically influenced their adaptation to biotic and abiotic stress. The rapid development multi‐omics approaches greatly improved our understanding the diversity, composition, functions plant microbiomes, but how global climate change affects assembly microbiomes roles in regulating host changing environmental conditions is not fully known. In review, we summarize recent advancements community responses factors such as elevated CO 2 levels, warming, drought. We further delineate research trends hotspots plant–microbiome interactions context change, key mechanisms by which influence climate. propose that future urgently needed unravel impact genes signal molecules modulated on communities, elucidate evolutionary response plant–microbe at level, engineer synthetic mitigate effects fitness.

Language: Английский

Citations

0