Melon CmRLCK VII‐8 kinase genes CmRLCK27, CmRLCK30 and CmRLCK34 modulate resistance against bacterial and fungal diseases in Arabidopsis DOI

Ya Wen,

Fahao Wang, Hui Wang

et al.

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(4)

Published: July 1, 2024

Abstract Receptor‐like cytoplasmic kinases (RLCKs) represent a distinct class of receptor‐like crucial for various aspects plant biology, including growth, development, and stress responses. This study delves into the characterization RLCK VII‐8 members within cucurbits, particularly in melon, examining both structural features phylogenetic relationships these genes/proteins. The investigation extends to their potential involvement disease resistance by employing ectopic overexpression Arabidopsis . promoters CmRLCK genes harbor multiple phytohormone‐ stress‐responsive cis ‐acting elements, with majority (excluding CmRLCK39 ) displaying upregulated expression response defense hormones fungal infection. Subcellular localization studies reveal that proteins predominantly reside on plasma membrane, CmRLCK29 CmRLCK30 exhibiting additional nuclear distribution. Notably, plants overexpressing manifest dwarfing delayed flowering phenotypes. Overexpression CmRLCK27 , CmRLCK34 imparts enhanced against Botrytis cinerea Pseudomonas syringae pv. tomato DC3000, concomitant strengthened reactive oxygen species accumulation. actively participate chitin‐ flg22‐triggered immune Furthermore, interacts CmMAPKKK1 CmARFGAP, adding layer complexity regulatory network. In summary, this functional underscores roles responses influencing pathogen‐induced gene ROS

Language: Английский

Blocking the isoflavone chemoreceptor in Phytophthora sojae to prevent disease DOI Creative Commons
Peiyun Ji, Yazhou Bao, Hao Zhou

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(2)

Published: Jan. 8, 2025

Inhibiting pathogen chemotaxis is a promising strategy for reducing disease pressure. However, this currently in the proof-of-concept stage. Here, Phytophthora sojae was used as model, its biflagellated zoospores could sense genistein, soybean root exudate, to navigate host and initiate infection. We identify P. IRK1 (isoflavone-insensitive receptor kinase 1) with PsIRK2 functioning coreceptor that enhances binding affinity of PsIRK1 genistein regulates by phosphorylating G protein α subunit. Last, we an antagonist, esculetin, which disrupts PsIRK1-genistein interaction, thereby preventing infection repelling zoospores. Our findings reveal mechanism senses demonstrate prevention targeting chemoreceptor.

Language: Английский

Citations

1
Receptor-like proteins: decision-makers of plant immunity DOI Creative Commons

Minrui Cai,

Hongqiang Yu,

E Sun

et al.

Phytopathology Research, Journal Year: 2024, Volume and Issue: 6(1)

Published: Nov. 14, 2024

Abstract Receptor-like proteins (RLPs) are crucial pattern-recognition receptors on the surface of plant cells, which involved in almost all processes life cycle. Recently, evolution high-throughput sequencing technology has strengthened appraisal and identification increasing numbers RLPs primarily improved our understanding roles various biological processes. Here, we review classification evolutionary characteristics their regulatory pattern-triggered immunity (PTI) effector-triggered (ETI). In particular, summarize ligands recognized by RLPs, co-receptors, downstream signalling cascades mediated RLPs. To summarize, this offers beneficial guidance for researchers at-a-glance comprehension function It also puts forward prospect mining broad-spectrum candidate genes light research disease resistance mechanism current challenges breeding.

Language: Английский

Citations

3
Mendelian randomization analysis and molecular mechanism study of childhood asthma and obstructive sleep apnea DOI
Xinyu Wang, Long Zhang, Hao Chen

et al.

Human Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Language: Английский

Citations

0
Reciprocal phosphorylation between SOAK1 and SOBIR1 fine-tunes receptor-like protein (RLP)–mediated plant immunity DOI Creative Commons

Yongming Chen,

Yingying Song,

Zhipeng Tu

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(16)

Published: April 18, 2025

SUPPRESSOR OF BIR1-1 (SOBIR1) is a receptor-like kinase (RLK) that acts as coreceptor for multiple proteins (RLPs) to mediate pathogen-associated molecular pattern)–triggered immunity. However, the regulation of SOBIR1 homeostasis and activity remains largely unknown. Our study reveals SOBIR1-ASSOCIATED PROTEIN KINASE 1 (SOAK1), member cytoplasmic (RLCK)-V subfamily with transmembrane domain, negatively regulates RLP-mediated immune responses. SOAK1 constitutively interacts modulates SOBIR1-dependent signaling. directly phosphorylates at serine-406, substantially impairing its ability transphosphorylate itself BAK1. The conservation serine-406 residue among various flowering plants suggests phosphorylation this site plays critical role in regulating plant Conversely, also primarily serine-73, inhibiting SOAK1’s derepressing activity. This elucidates regulatory mechanism highlights an uncharacterized RLCK-V members

Language: Английский

Citations

0
A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance DOI Creative Commons
Shuai Zhang, Ruolan Li, Wei Fan

et al.

Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(11)

Published: Nov. 1, 2024

Abstract Ciboria shiraiana is a necrotrophic fungus that causes mulberry sclerotinia disease resulting in huge economic losses agriculture. During infection, the uses immunity elicitors to induce plant tissue necrosis could facilitate its colonization on plants. However, key and immune mechanisms remain unclear C. . Herein, novel elicitor Cs08297 secreted by was identified, it found target apoplast plants cell death. cysteine‐rich protein unique , cysteine residues were crucial for ability induced series of defence responses Nicotiana benthamiana including burst reactive oxygen species (ROS), callose deposition, activation defence‐related genes. induced‐cell death mediated leucine‐rich repeat (LRR) receptor‐like kinases BAK1 SOBIR1. Purified His‐tagged Cs08297‐thioredoxin fusion triggered different enhanced resistance diseases. necessary sclerotial development, oxidative‐stress adaptation, wall integrity but negatively regulated virulence In conclusion, our results revealed fungal fungi inducing immunity. Furthermore, potential enhance provides new control agricultural diseases biologically.

Language: Английский

Citations

1
A Receptor Like Cytoplasmic Kinase evolved in Aeschynomene legumes to mediate Nod-independent rhizobial symbiosis DOI
Natasha Horta Araújo, David Landry, Johan Quilbé

et al.

Published: July 14, 2024

Abstract Many plants interact symbiotically with arbuscular mycorrhizal (AM) fungi to enhance inorganic phosphorus uptake, and legumes also develop a nodule symbiosis rhizobia for nitrogen acquisition. Establishment functioning of both symbioses rely on common plant signaling pathway activated by structurally related Myc- Nod-factors. Recently, SPARK Receptor-like-Kinase (RLK)/Receptor-like Cytoplasmic Kinase (RLCK) complex was shown be essential AM in monocot dicot plants. Here, we show that Aeschynomene the RLCK component this receptor has evolved following gene duplication event mediates unique is independent rhizobial Nod factors. In evenia , AeRLCK2 crucial initiation but not AM. Additionally, physically interacts phosphorylated Cysteine-rich RLK, AeCRK, required nodulation. This work reveals novel evolutionary origin Nod-independent from

Language: Английский

Citations

0
Melon CmRLCK VII‐8 kinase genes CmRLCK27, CmRLCK30 and CmRLCK34 modulate resistance against bacterial and fungal diseases in Arabidopsis DOI

Ya Wen,

Fahao Wang, Hui Wang

et al.

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(4)

Published: July 1, 2024

Abstract Receptor‐like cytoplasmic kinases (RLCKs) represent a distinct class of receptor‐like crucial for various aspects plant biology, including growth, development, and stress responses. This study delves into the characterization RLCK VII‐8 members within cucurbits, particularly in melon, examining both structural features phylogenetic relationships these genes/proteins. The investigation extends to their potential involvement disease resistance by employing ectopic overexpression Arabidopsis . promoters CmRLCK genes harbor multiple phytohormone‐ stress‐responsive cis ‐acting elements, with majority (excluding CmRLCK39 ) displaying upregulated expression response defense hormones fungal infection. Subcellular localization studies reveal that proteins predominantly reside on plasma membrane, CmRLCK29 CmRLCK30 exhibiting additional nuclear distribution. Notably, plants overexpressing manifest dwarfing delayed flowering phenotypes. Overexpression CmRLCK27 , CmRLCK34 imparts enhanced against Botrytis cinerea Pseudomonas syringae pv. tomato DC3000, concomitant strengthened reactive oxygen species accumulation. actively participate chitin‐ flg22‐triggered immune Furthermore, interacts CmMAPKKK1 CmARFGAP, adding layer complexity regulatory network. In summary, this functional underscores roles responses influencing pathogen‐induced gene ROS

Language: Английский

Citations

0