Size control and oscillations of active droplets in synthetic cells DOI Creative Commons
Job Boekhoven,

J I de Diego Sastre,

Advait Thatte

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Abstract Oscillations in the formation and dissolution of molecular assemblies inside living cells are pivotal orchestrating various cellular functions processes. However, designing such rhythmic patterns synthetic remains a challenge. Here, we demonstrate spontaneous emergence spatio-temporal oscillations number droplets, size, position within cell. The coacervate-based droplets these sediment fuse at cell’s bottom. Through newly discovered size control mechanism, sedimented, large shrink by expelling droplet material. expelled molecules nucleate new top cell, which grow again. These sustained converting chemical fuel into waste can continue for hundreds periods without evidence fatigue. Strikingly, period oscillation is minute's regime tunable. findings bring us closer to creating more life-like materials de novo life.

Language: Английский

A roadmap toward the synthesis of life DOI Creative Commons
Christine M. E. Kriebisch, Olga Bantysh, Lorena Baranda Pellejero

et al.

Chem, Journal Year: 2025, Volume and Issue: unknown, P. 102399 - 102399

Published: Feb. 1, 2025

Language: Английский

Citations

3
A Prebiotic Precursor to Life’s Phosphate Transfer System with an ATP Analog and Histidyl Peptide Organocatalysts DOI Creative Commons
Oliver R. Maguire, Iris B. A. Smokers,

Bob G. Oosterom

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(11), P. 7839 - 7849

Published: March 6, 2024

Biochemistry is dependent upon enzyme catalysts accelerating key reactions. At the origin of life, prebiotic chemistry must have incorporated catalytic While this would yielded much needed amplification certain reaction products, it come at possible cost rapidly depleting high energy molecules that acted as chemical fuels. solves problem by combining kinetically stable and thermodynamically activated (e.g., ATP) with catalysts. Here, we demonstrate a phosphate transfer system involving an ATP analog (imidazole phosphate) histidyl peptides, which function organocatalytic analogs. We peptides catalyze phosphorylations via phosphorylated intermediate. integrate these histidyl-catalyzed into complete scenario whereby inorganic organic compounds though physicochemical wet-dry cycles. Our work demonstrates plausible for catalyzed production on early Earth how precursors, could played important role in this.

Language: Английский

Citations

10
Size control and oscillations of active droplets in synthetic cells DOI Creative Commons

Judit Sastre,

Advait Thatte,

Alexander M. Bergmann

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 26, 2025

Abstract Oscillations in the formation and dissolution of molecular assemblies inside living cells are pivotal orchestrating various cellular functions processes. However, designing such rhythmic patterns synthetic remains a challenge. Here, we demonstrate spontaneous emergence spatio-temporal oscillations number droplets, size, their spatial distribution within cell. The coacervate-based droplets these sediment fuse at cell’s bottom. Through size control mechanism, sedimented, large shrink by expelling droplet material. expelled molecules nucleate new top cell, which grow again. These sustained converting chemical fuel into waste can continue for hundreds periods without evidence fatigue. Strikingly, period oscillation is minute’s regime tunable. design oscillating artificial organelles brings us closer to creating more life-like materials de novo life.

Language: Английский

Citations

0
Size control and oscillations of active droplets in synthetic cells DOI Creative Commons

Judit Sastre,

Advait Thatte,

Alexander M. Bergmann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract Oscillations in the formation and dissolution of molecular assemblies inside living cells are pivotal orchestrating various cellular functions processes. However, designing such rhythmic patterns synthetic remains a challenge. Here, we demonstrate spontaneous emergence spatio-temporal oscillations number droplets, size, position within cell. The coacervate-based droplets these sediment fuse at cell’s bottom. Through newly discovered size control mechanism, sedimented, large shrink by expelling droplet material. expelled molecules nucleate new top cell, which grow again. These sustained converting chemical fuel into waste can continue for hundreds periods without evidence fatigue. Strikingly, period oscillation is minute’s regime tunable. design oscillating organelles brings us closer to creating more life-like materials de novo life.

Language: Английский

Citations

1
Coacervate Droplets as Biomimetic Models for Designing Cell‐Like Microreactors DOI Creative Commons
Tsvetomir Ivanov, Thao P. Doan‐Nguyen,

Mohammed A. Belahouane

et al.

Macromolecular Rapid Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Abstract Coacervates are versatile compartments formed by liquid–liquid phase separation. Their dynamic behavior and molecularly crowded microenvironment make them ideal materials for creating cell‐like systems such as synthetic cells microreactors. Recently, combinations of natural molecules have been exploited via simple or complex coacervation to create that can be used build hierarchical chemical with life‐like properties. This review highlights recent advances in the design coacervate their application biomimetic reactors cell mimicking systems. It first explores variety influence structure on controlled behavior. Then, applications coacervates reviewed, focusing how they microreactors through ability sequester provide a distinct regulatory reactions aqueous media.

Language: Английский

Citations

1
Life in Lab: Chemically Fueled Systems Chemistry for Emergent Properties DOI Creative Commons
Sudeep Koppayithodi,

Prerna Ranasingh,

Nishant Singh

et al.

ChemSystemsChem, Journal Year: 2024, Volume and Issue: 6(6)

Published: July 9, 2024

Abstract Understanding the emergence of complex properties in dissipative non‐equilibrium systems is crucial for unraveling mysteries life processes. The review focuses on documented research chemically fueled autonomous systems, self‐sorting towards compartmentalization, self‐replication via autocatalysis, and rhythmic chemical oscillators. In addition to that, also discusses newly introduced reactions dynamic combinatorial libraries systems.

Language: Английский

Citations

0
Regulating nucleic acid catalysis using active droplets DOI Creative Commons

Anna-Lena Holtmannspötter,

Corbin Machatzke,

Christian Begemann

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(47)

Published: Aug. 9, 2024

Abstract Cells use transient membraneless organelles to regulate biological reaction networks. For example, stress granules selectively store mRNA downregulate protein expression in response heat or oxidative stress. Models mimicking this active behavior should be established better understand vivo regulation involving compartmentalization. Here we active, complex coacervate droplets as a model for spatiotemporally control the activity of catalytic DNA (DNAzyme). Upon partitioning into these peptide‐RNA droplets, DNAzyme unfolds and loses its ability catalyze cleavage nucleic acid strand. We can transiently pause upon inducing droplet formation with fuel. After fuel consumption, autonomously restarts. envision system could used up multiple reactions network, helping complexity cell‘s pathways. By creating network where reciprocally properties, would have powerful tool engineering synthetic cells.

Language: Английский

Citations

0
Regulating nucleic acid catalysis using active droplets DOI Creative Commons

Anna-Lena Holtmannspötter,

Corbin Machatzke,

Christian Begemann

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(47)

Published: Aug. 9, 2024

Abstract Zellen benutzen transiente, membranlose Organellen um biologische Reaktionsnetzwerke zu regulieren. Stressgranula zum Beispiel sind in der Lage, selektiv mRNA einzulagern, die Proteinexpression im Fall von Hitze oder oxidativem Stress Modelle welches diese aktive Verhalten nachstellen nützlich vivo Regulierung durch Kompartimentierung besser verstehen. Hier wir komplexe Koazervat‐Tröpfchen als Modell für Organellen, welche Aktivität einer katalytischen DNA (DNAzym) räumlich und zeitlich kontrollieren können. Beim Eintritt Peptid‐RNA‐Tröpfchen entfaltet sich das DNAzym verliert seine Fähigkeit, einen Nukleinsäurestrang spalten. Wir können vorübergehend pausieren, indem Tröpfchenbildung mit einem Treibstoff induzieren. Nach dem Verbrauch des Treibstoffs beginnt wieder selbst. uns vorstellen, dass dieses System zur Hoch‐ Herunterregulierung mehrere Reaktionen Netzwerk benutzt werden könnte, was besseren Verständnis Komplexität zellulären Reaktionswegen führen würde. Mit Netzwerk, welchem reziprok Eigenschaften Tröpfchens regulieren kann, hätten ein vielversprechendes Instrument Konstruktion synthetischen Zellen.

Citations

0
Size control and oscillations of active droplets in synthetic cells DOI Creative Commons
Job Boekhoven,

J I de Diego Sastre,

Advait Thatte

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Abstract Oscillations in the formation and dissolution of molecular assemblies inside living cells are pivotal orchestrating various cellular functions processes. However, designing such rhythmic patterns synthetic remains a challenge. Here, we demonstrate spontaneous emergence spatio-temporal oscillations number droplets, size, position within cell. The coacervate-based droplets these sediment fuse at cell’s bottom. Through newly discovered size control mechanism, sedimented, large shrink by expelling droplet material. expelled molecules nucleate new top cell, which grow again. These sustained converting chemical fuel into waste can continue for hundreds periods without evidence fatigue. Strikingly, period oscillation is minute's regime tunable. findings bring us closer to creating more life-like materials de novo life.

Language: Английский

Citations

0