Photobiocatalytic methane hydroxylation driven by organosilica nanodots DOI
Jinha Jang,

Rowina Lestari,

Yonghan Jo

et al.

Applied Catalysis B Environment and Energy, Journal Year: 2024, Volume and Issue: 365, P. 124999 - 124999

Published: Dec. 28, 2024

Language: Английский

MmoD and MmoG Are Crucial for the Synthesis of Soluble Methane Monooxygenase in Methanotrophs DOI
Minggen Cheng, Yongchuang Liu, Xin Yan

et al.

Molecular Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

ABSTRACT Soluble methane monooxygenase (sMMO) from methanotrophs has been extensively investigated for decades. However, major knowledge gaps persist regarding the synthesis mechanism of sMMO, particularly concerning ambiguous roles mmoD and mmoG in sMMO gene cluster. Here, functions were a model methanotrophic strain, Methylotuvimicrobium buryatense 5GB1C. Both genes found to be essential functional expression sMMO. Genetic biochemical data supported hypothesis that MmoG acts as folding chaperone both MmoX MmoR, while MmoD serves an assembly hydroxylase component. The Escherichia coli was achieved mmoD‐ mmoG‐ dependent manner. In addition, deletion dramatically reduced transcription cluster M. 5GB1C, implying may regulate via unknown mechanism. Knockout neither nor abolished feature “copper switch”, indicating they do not serve initial regulators switch”. These results demonstrate crucial offer new insights into heterologous

Language: Английский

Citations

0
Advancements in Biological Conversion of C1 Feedstocks: Sustainable Bioproduction and Environmental Solutions DOI
Wooyoung Park,

Sungman Cha,

Ji‐Sook Hahn

et al.

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

The use of one-carbon (C1) feedstocks, including carbon dioxide (CO

Language: Английский

Citations

2
Recent Insights into the Reaction Mechanisms of Non‐Heme Diiron Enzymes Containing Oxoiron(IV) Complexes DOI Open Access
Ruining Li, Shi‐Lu Chen

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Oxoiron(IV) complexes are key intermediates in the catalytic reactions of some non-heme diiron enzymes. These enzymes, across various subfamilies, activate dioxygen to generate high-valent diiron-oxo species, which, turn, drive activation substrates and mediate a variety challenging oxidative transformations. In this review, we summarize structures, formation mechanisms, functions eight representative enzymes (sMMO, RNR, ToMO, MIOX, PhnZ, SCD1, AlkB, SznF) spanning five subfamilies. We also categorize analyze structural mechanistic differences among these

Language: Английский

Citations

0
Photobiocatalytic methane hydroxylation driven by organosilica nanodots DOI
Jinha Jang,

Rowina Lestari,

Yonghan Jo

et al.

Applied Catalysis B Environment and Energy, Journal Year: 2024, Volume and Issue: 365, P. 124999 - 124999

Published: Dec. 28, 2024

Language: Английский

Citations

0