N7-methyladenosine-induced SLC7A7 serves as a prognostic biomarker in pan-cancer and promotes CRC progression in colorectal cancer DOI Creative Commons
Fuqi Wang,

Shiqian Zhang,

Zhuang Chen

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 28, 2024

Abstract Solute transport family 7A member 7 (SLC7A7) mutations contribute to lysinuric protein intolerance (LPI), which is the mechanism of action that has been extensively studied. In colorectal cancer (CRC), SLC7A7 appears play a role, but features and mechanisms are not yet well understood. Survival was analyzed using Kaplan–Meier analysis. Enrichment analysis performed characterize, immune infiltration, methylation, genetic instability, crucial pathways SLC7A7. Afterward, functional experiments were conducted in vitro investigate how affects tumor metastasis. Mechanistically, quantitative real-time PCR (qRT-PCR), western blot (WB), methylated RNA immunoprecipitation (me-RIP) carried out confirm methylation modification related functions. High levels expression predictive worse prognosis for CRC patients. showed significantly enriched during EMT could be Wnt/β-catenin signaling pathway, infiltration pan-cancer macrophages, affected specific cancers. indicated promote migration invasion cells experiments. observed potentially interact with possibly by influencing adenomatous polyposis coli (APC) expression. Furthermore, we identified undergoes N7-methylguanosine (m7G) modification, may regulate mRNA stability, Quaking (QKI) playing role this process recognizing m7G modification. Our results indicate metastasis through SLC7A7/APC/Wnt/β-catenin pathway. Moreover, might involved regulating highlighting novel layer regulation.

Language: Английский

Evolution and developmental functions of the dystrophin-associated protein complex: beyond the idea of a muscle-specific cell adhesion complex DOI Creative Commons
Vincent Mirouse

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: June 13, 2023

The Dystrophin-Associated Protein Complex (DAPC) is a well-defined and evolutionarily conserved complex in animals. DAPC interacts with the F-actin cytoskeleton via dystrophin, extracellular matrix membrane protein dystroglycan. Probably for historical reasons that have linked its discovery to muscular dystrophies, function often described as limited muscle integrity maintenance by providing mechanical robustness, which implies strong cell-extracellular adhesion properties. In this review, phylogenetic functional data from different vertebrate invertebrate models will be analyzed compared explore molecular cellular functions of DAPC, specific focus on dystrophin. These reveals evolution paths cells are not intrinsically many features dystrophin domains been identified yet. adhesive properties also discussed reviewing available evidence common key complexes, such clustering, force transmission, mechanosensitivity mechanotransduction. Finally, review highlights developmental roles tissue morphogenesis basement (BM) assembly may indicate adhesion-independent functions.

Language: Английский

Citations

13
Wnt signaling pathway in spinal cord injury: from mechanisms to potential applications DOI Creative Commons
Kai Li,

Zanzhi Chen,

Xuejing Chang

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: July 24, 2024

Spinal cord injury (SCI) denotes damage to both the structure and function of spinal cord, primarily manifesting as sensory motor deficits caused by disruptions in neural transmission pathways, potentially culminating irreversible paralysis. Its pathophysiological processes are complex, with numerous molecules signaling pathways intricately involved. Notably, pronounced upregulation Wnt pathway post-SCI holds promise for regeneration repair. Activation plays a crucial role neuronal differentiation, axonal regeneration, local neuroinflammatory responses, cell apoptosis, highlighting its potential therapeutic target treating SCI. However, excessive activation can also lead negative effects, need further investigation into applicability significance This paper provides an overview latest research advancements SCI, summarizing recent progress treatment strategies associated analyzing their advantages disadvantages. Additionally, we offer insights clinical application along prospective avenues future direction.

Language: Английский

Citations

2
Constitutive sodium permeability in a C. elegans two-pore domain potassium channel DOI Creative Commons
Olga Andrini,

Ismail Ben Soussia,

Philippe Tardy

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(43)

Published: Oct. 15, 2024

Two-pore domain potassium (K2P) channels play a central role in modulating cellular excitability and neuronal function. The unique structure of the selectivity filter K2P other determines their ability to allow selective passage ions across cell membranes. nematode C. elegans has one largest families, with 47 subunit-coding genes. This remarkable expansion been accompanied by evolution atypical sequences that diverge from canonical TxGYG motif. Whether how this sequence variation may impact function not investigated so far. Here, we show UNC-58 channel is constitutively permeable sodium cysteine residue its responsible for behavior. Indeed, performing vivo electrophysiological recordings Ca 2+ imaging experiments, demonstrate depolarizing effect muscles sensory neurons. Consistently, unc-58 gain-of-function mutants are hypercontracted, unlike relaxed phenotype observed hyperactive many neuromuscular channels. Finally, combining molecular dynamics simulations functional studies Xenopus laevis oocytes, plays key unconventional permeability UNC-58. As predicting consequences variations silico remains major challenge, our study illustrates experiments essential determine contribution such unusual electrical profile excitable cells.

Language: Английский

Citations

1
Structural and Functional Insights into Dishevelled-Mediated Wnt Signaling DOI Creative Commons
Lei Wang,

Rui Zhu,

Zehua Wen

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(22), P. 1870 - 1870

Published: Nov. 11, 2024

Dishevelled (DVL) proteins precisely control Wnt signaling pathways with many effectors. While substantial research has advanced our understanding of DVL's role in pathways, key questions regarding its regulatory mechanisms and interactions remain unresolved. Herein, we present the recent advances perspectives on how DVL regulates signaling. The experimentally determined conserved domain structures conjunction AlphaFold-predicted are used to understand regulation. We also summarize various diseases provide insights into further directions for DVL-mediated mechanisms. These findings underscore importance as a pharmaceutical target or biological marker diseases, offering exciting potential future biomedical applications.

Language: Английский

Citations

1
N7-methyladenosine-induced SLC7A7 serves as a prognostic biomarker in pan-cancer and promotes CRC progression in colorectal cancer DOI Creative Commons
Fuqi Wang,

Shiqian Zhang,

Zhuang Chen

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 28, 2024

Abstract Solute transport family 7A member 7 (SLC7A7) mutations contribute to lysinuric protein intolerance (LPI), which is the mechanism of action that has been extensively studied. In colorectal cancer (CRC), SLC7A7 appears play a role, but features and mechanisms are not yet well understood. Survival was analyzed using Kaplan–Meier analysis. Enrichment analysis performed characterize, immune infiltration, methylation, genetic instability, crucial pathways SLC7A7. Afterward, functional experiments were conducted in vitro investigate how affects tumor metastasis. Mechanistically, quantitative real-time PCR (qRT-PCR), western blot (WB), methylated RNA immunoprecipitation (me-RIP) carried out confirm methylation modification related functions. High levels expression predictive worse prognosis for CRC patients. showed significantly enriched during EMT could be Wnt/β-catenin signaling pathway, infiltration pan-cancer macrophages, affected specific cancers. indicated promote migration invasion cells experiments. observed potentially interact with possibly by influencing adenomatous polyposis coli (APC) expression. Furthermore, we identified undergoes N7-methylguanosine (m7G) modification, may regulate mRNA stability, Quaking (QKI) playing role this process recognizing m7G modification. Our results indicate metastasis through SLC7A7/APC/Wnt/β-catenin pathway. Moreover, might involved regulating highlighting novel layer regulation.

Language: Английский

Citations

0