Molecular mechanisms of escrt-mediated autophagosome maturation in plants DOI
Niccolò Mosesso,

Erika Isono

Autophagy, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 2

Published: Oct. 31, 2024

Diverse environmental stress factors affect the functionality of proteins and membrane compartments within cells causing potentially irremediable damage to cell. A major process eliminate nonfunctional molecular aggregates or damaged organelles under conditions is macroautophagy/autophagy, thus making its regulation critical for cellular adaptation survival. The formation autophagosomes coordinated by a wide range culminates in closure cup-shaped double phagophore. endosomal sorting complex required transport (ESCRT) machinery has been proposed mediate sealing autophagic membranes. However, basis ESCRT recruitment phagophores are not yet fully understood. We recently described role ALIX (ALG-2 interacting protein-X) interactor CALB1 (Ca2+-dependent Lipid Binding protein 1) autophagosome maturation during salt Arabidopsis. Our study shows that important phagophore subsequent delivery vacuole. localizes on salt-induced together with ALIX. stimulates phase separation ALIX, which can facilitate further

Language: Английский

Biomolecular condensates at the plasma membrane: Insights into plant cell signaling DOI Creative Commons

Punita Lalchand,

Didier‐Deschamps Ashley,

Xue Pan

et al.

Current Opinion in Plant Biology, Journal Year: 2025, Volume and Issue: 84, P. 102697 - 102697

Published: Feb. 24, 2025

Biomolecular condensates, often formed through liquid-liquid phase separation (LLPS), are increasingly recognized as a critical mechanism for cellular compartmentalization across diverse biological systems. Although traditionally considered membrane-less entities, recent discoveries highlight their dynamic interactions with membranes, where they regulate various processes, including signal transduction. Signaling lipids observed in condensates. Despite these advancements, our understanding of such condensates plant biology remains limited. This review highlights studies involving membrane-associated plants, focusing particularly on the plasma membrane (PM) and potential roles PM-based signaling.

Language: Английский

Citations

1
Multifaceted roles of the ATG8 protein family in plant autophagy: from autophagosome biogenesis to cargo recognition DOI Creative Commons
Yixin Wu, Rui Xu, Xiaohong Zhuang

et al.

Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 168981 - 168981

Published: Feb. 1, 2025

Language: Английский

Citations

0
The Role of Membrane-Biomolecular Condensate Interactions in Stress DOI Creative Commons
Konstantin Kutashev, Panagiotis N. Moschou

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100810 - 100810

Published: March 1, 2025

Language: Английский

Citations

0
The Green ESCRTs: Newly Defined Roles for ESCRT Proteins in Plants DOI Creative Commons
Ethan Weiner, Elizabeth Berryman, Ariadna González‐Solís

et al.

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108465 - 108465

Published: March 1, 2025

Language: Английский

Citations

0
Liquid-liquid phase separation regulates gene expression in plants DOI Creative Commons
Diyi Fu, Bochen Jiang

Agriculture Communications, Journal Year: 2025, Volume and Issue: unknown, P. 100084 - 100084

Published: May 1, 2025

Citations

0
Biomolecular condensation of ERC1 recruits ATG8 and NBR1 to drive autophagosome formation for plant heat tolerance DOI
Ka Kit Chung,

Ziwei Zhao,

Kai Ching Law

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Macroautophagy (hereafter autophagy) is essential for cells to respond nutrient stress by delivering cytosolic contents vacuoles degradation via the formation of a multi-layer vesicle named autophagosome. A set autophagy-related (ATG) regulators are recruited phagophore assembly site initiation phagophore, as well its expansion and closure subsequent delivery into vacuole. However, it remains elusive that how regulated under different conditions. Here, we described an unknown

Language: Английский

Citations

0
How coat proteins shape autophagy in plant cells DOI
Taijoon Chung, Ye Eun Choi, Kyoungjun Song

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

Abstract Autophagy is a membrane trafficking pathway through which eukaryotic cells target their own cytoplasmic constituents for degradation in the lytic compartment. Proper biogenesis of autophagic organelles requires conserved set autophagy-related (ATG) proteins and interacting factors, such as signalling phospholipid phosphatidylinositol 3-phosphate (PI3P) coat complex II (COPII). The COPII machinery, was originally identified involved formation vesicles budding from endoplasmic reticulum, contributes to initiation yeast, metazoan, plant cells; however, exact mechanisms remain elusive. Recent studies using model species Arabidopsis thaliana have revealed that plant-specific PI3P effectors are autophagy. effector FYVE2 interacts with ATG18 components, indicating an additional role machinery later stages autophagosome biogenesis. In this Update, we examined recent research on proposed working models functions autophagy, including its potential roles stabilizing curvature sealing phagophore.

Language: Английский

Citations

0
Molecular mechanisms of escrt-mediated autophagosome maturation in plants DOI
Niccolò Mosesso,

Erika Isono

Autophagy, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 2

Published: Oct. 31, 2024

Diverse environmental stress factors affect the functionality of proteins and membrane compartments within cells causing potentially irremediable damage to cell. A major process eliminate nonfunctional molecular aggregates or damaged organelles under conditions is macroautophagy/autophagy, thus making its regulation critical for cellular adaptation survival. The formation autophagosomes coordinated by a wide range culminates in closure cup-shaped double phagophore. endosomal sorting complex required transport (ESCRT) machinery has been proposed mediate sealing autophagic membranes. However, basis ESCRT recruitment phagophores are not yet fully understood. We recently described role ALIX (ALG-2 interacting protein-X) interactor CALB1 (Ca2+-dependent Lipid Binding protein 1) autophagosome maturation during salt Arabidopsis. Our study shows that important phagophore subsequent delivery vacuole. localizes on salt-induced together with ALIX. stimulates phase separation ALIX, which can facilitate further

Language: Английский

Citations

0