Effects of Stress Exposure to Pain Perception in Pre-Clinical Studies: Focus on the Nociceptin/Orphanin FQ–NOP Receptor System DOI Creative Commons

P. Pola,

Alessia Frezza,

Elaine C. Gavioli

et al.

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(9), P. 936 - 936

Published: Sept. 19, 2024

Exposure to physical and psychological stress modulates pain transmission in a dual manner. Stress-induced analgesia (SIA) refers the reduction sensitivity that can occur response acute stress. On contrary, chronic exposure may lead phenomenon named stress-induced hyperalgesia (SIH). SIH is clinically relevant since it has been well documented exacerbates patients with several syndromes, including migraine. The availability of animal models SIA high importance for understanding biological mechanisms leading these phenomena identification pharmacological targets useful alleviate burden stress-exacerbated pain. Among targets, nociceptin/orphanin FQ (N/OFQ)–N/OFQ peptide (NOP) receptor system identified as key modulator both susceptibility. This review describes first experimental approaches induce rodents. second part manuscript summarizes scientific evidence suggests N/OFQ–NOP player stress–pain interaction candidates NOP antagonists drugs mitigate detrimental effects on perception.

Language: Английский

Lights, fiber, action! A primer on in vivo fiber photometry DOI Creative Commons
Eleanor H. Simpson, Thomas Akam, Tommaso Patriarchi

et al.

Neuron, Journal Year: 2023, Volume and Issue: 112(5), P. 718 - 739

Published: Dec. 15, 2023

Fiber photometry is a key technique for characterizing brain-behavior relationships in vivo. Initially, it was primarily used to report calcium dynamics as proxy neural activity via genetically encoded indicators. This generated new insights into brain functions including movement, memory, and motivation at the level of defined circuits cell types. Recently, opportunity discovery with fiber has exploded development an extensive range fluorescent sensors biomolecules neuromodulators peptides that were previously inaccessible critical advance, combined availability affordable "plug-and-play" recording systems, made monitoring molecules high spatiotemporal precision during behavior highly accessible. However, while opening exciting avenues research, rapid expansion applications occurred without coordination or consensus on best practices. Here, we provide comprehensive guide help end-users execute, analyze, suitably interpret studies.

Language: Английский

Citations

54
A high-performance GRAB sensor reveals differences in the dynamics and molecular regulation between neuropeptide and neurotransmitter release DOI Creative Commons
Xiju Xia, Yulong Li

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 18, 2025

The co-existence and co-transmission of neuropeptides small molecule neurotransmitters within individual neuron represent a fundamental characteristic observed across various species. However, the differences regarding their in vivo spatiotemporal dynamics underlying molecular regulation remain poorly understood. Here, we develop GPCR-activation-based (GRAB) sensor for detecting short neuropeptide F (sNPF) with high sensitivity resolution. Furthermore, investigate between sNPF acetylcholine (ACh) from same neurons. Interestingly, our findings reveal distinct release ACh. Notably, results indicate that synaptotagmins (Syt) are involved these two processes, as Syt7 Sytα release, while Syt1 ACh release. Thus, this high-performance GRAB provides robust tool studying shedding insights into unique distinguish neurotransmitters.

Language: Английский

Citations

2
The lateral habenula: A hub for value-guided behavior DOI Creative Commons
Dominik Groos, Fritjof Helmchen

Cell Reports, Journal Year: 2024, Volume and Issue: 43(4), P. 113968 - 113968

Published: March 22, 2024

SummaryThe habenula is an evolutionarily highly conserved diencephalic brain region divided into two major parts, medial and lateral. Over the past decades, studies of lateral (LHb), in particular, have identified key functions value-guided behavior health disease. In this review, we focus on recent insights LHb connectivity its functional relevance for different types aversive appetitive behavior. First, give overview anatomical organization main cellular composition. Next, elaborate how distinct neuronal subpopulations encode stimuli their involvement more complex decision-making processes. Finally, scrutinize afferent efferent connections discuss implications LHb-dependent A deepened understanding circuit components will substantially contribute to our knowledge

Language: Английский

Citations

9
Current and emerging methods for probing neuropeptide transmission DOI
Tongrui Qian, Huan Wang, Xiju Xia

et al.

Current Opinion in Neurobiology, Journal Year: 2023, Volume and Issue: 81, P. 102751 - 102751

Published: July 22, 2023

Language: Английский

Citations

10
Noncanonical Amino Acid Tools and Their Application to Membrane Protein Studies DOI Creative Commons

Chiara De Faveri,

Jordan M. Mattheisen, Thomas P. Sakmar

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(22), P. 12498 - 12550

Published: Nov. 7, 2024

Methods rooted in chemical biology have contributed significantly to studies of integral membrane proteins. One recent key approach has been the application genetic code expansion (GCE), which enables site-specific incorporation noncanonical amino acids (ncAAs) with defined properties into Efficient GCE is challenging, especially for proteins, specialized biogenesis and cell trafficking machinery tend be expressed at low levels membranes. Many eukaryotic proteins cannot functionally

Language: Английский

Citations

3
The role of dorsal raphe nucleus neuropeptides in reward and aversion DOI Creative Commons

Kathryn Braden,

Daniel C. Castro

Frontiers in Behavioral Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: April 9, 2025

The dorsal raphe nucleus is a critical node for affective and motivated circuits in the brain. Though typically known as serotonergic hub, also highly enriched variety of neuropeptides. Recent advances biotechnology behavioral modeling have led to resurgence neuropeptide research, allowing investigators target unique peptide systems with unprecedented clarity. Here, we review discuss multiple consider how their activity may contribute reward aversion. While this not an exhaustive review, short overview will highlight many opportunities available refine our understanding By more thoroughly studying neuropeptides, reveal novel pathways design effective therapeutics tailor treatments millions patients.

Language: Английский

Citations

0
Mapping and decoding neuropeptide signaling networks in nervous system function DOI Creative Commons
Isabel Beets, Jan Watteyne

Current Opinion in Neurobiology, Journal Year: 2025, Volume and Issue: 92, P. 103027 - 103027

Published: April 21, 2025

Neuropeptides are widespread signaling molecules that central to brain function in all animals. Recent advances profiling their expression across neural circuits, conjunction with detailed biochemical characterization of interactions receptors, have made it feasible build brain-wide maps neuropeptide signaling. Here, we discuss how recent reconstructions networks, from mammalian regions nervous system-wide C. elegans, reveal conserved organizational features neuropeptidergic networks. Furthermore, review technical breakthroughs vivo sensors for peptide release, receptor binding, and intracellular bring a mechanistic understanding networks within experimental reach. Finally, describe the architecture can change throughout evolution or even lifetime individuals, which highlights complexities must be considered understand these modulate circuit activity behavior different contexts.

Language: Английский

Citations

0
A Bright Future? A Perspective on Class C GPCR Based Genetically Encoded Biosensors DOI Creative Commons

Margulan Otanuly,

Martin Kubitschke, Olivia Andrea Masseck

et al.

ACS Chemical Neuroscience, Journal Year: 2024, Volume and Issue: 15(5), P. 889 - 897

Published: Feb. 21, 2024

One of the major challenges in molecular neuroscience today is to accurately monitor neurotransmitters, neuromodulators, peptides, and various other biomolecules brain with high temporal spatial resolution. Only a comprehensive understanding neuromodulator dynamics, their release probability, distribution will unravel ultimate role cognition behavior. This Perspective offers an overview potential design strategies for class C GPCR-based biosensors. It briefly highlights current applications biosensors, primary focus on GPCRs unique structural characteristics compared GPCR subfamilies. The discussion insights into plausible future approaches biosensor development targeting members this specific subfamily. important note that, at stage, we are contemplating possibilities rather than presenting concrete guide, as pipeline still under development.

Language: Английский

Citations

0
Effects of Stress Exposure to Pain Perception in Pre-Clinical Studies: Focus on the Nociceptin/Orphanin FQ–NOP Receptor System DOI Creative Commons

P. Pola,

Alessia Frezza,

Elaine C. Gavioli

et al.

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(9), P. 936 - 936

Published: Sept. 19, 2024

Exposure to physical and psychological stress modulates pain transmission in a dual manner. Stress-induced analgesia (SIA) refers the reduction sensitivity that can occur response acute stress. On contrary, chronic exposure may lead phenomenon named stress-induced hyperalgesia (SIH). SIH is clinically relevant since it has been well documented exacerbates patients with several syndromes, including migraine. The availability of animal models SIA high importance for understanding biological mechanisms leading these phenomena identification pharmacological targets useful alleviate burden stress-exacerbated pain. Among targets, nociceptin/orphanin FQ (N/OFQ)–N/OFQ peptide (NOP) receptor system identified as key modulator both susceptibility. This review describes first experimental approaches induce rodents. second part manuscript summarizes scientific evidence suggests N/OFQ–NOP player stress–pain interaction candidates NOP antagonists drugs mitigate detrimental effects on perception.

Language: Английский

Citations

0