High Selectivity Fluorescence and Electrochemical Dual-Mode Detection of Glutathione in the Serum of Parkinson’s Disease Model Mice and Humans

Analytical Chemistry, Journal Year: 2025, Volume and Issue: 97(2), P. 1318 - 1328

Published: Jan. 9, 2025

Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by the loss of dopaminergic neurons and accumulation alpha-synuclein. Glutathione (GSH), key antioxidant, significantly depleted in PD patients. This study presents dual-mode detection strategy for selectively determining GSH using single probe. A series "turn-on" electrochemical fluorescent probes were developed, with resorufin (Re) serving as reporting unit featuring specific recognition sites. Among these, 7-(3,5-dinitrophenoxy)-3H-phenoxazin-3-one (Re-DNP) probe was selected its high selectivity both Its response to superior comparison that observed hydrogen sulfide (H2S) cysteine (Cys). For screen-printed carbon electrode (SPCE)/carbon nanotube (CNT) modified electrodes, limit 5 μM, linear range 25-500 μM. In fluorescence detection, produced 78-fold increase emission at 630 nm presence GSH, strong correlation between intensity concentration 10-700 2 When applied real clinical serum samples, demonstrated lower levels mice human patients compared healthy controls. method provides sensitive accurate tool potential applications understanding GSH's role related diseases.

Language: Английский

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Response to the letter “Reassessing the Hypothesis of Essential Tremor as a Prodromal Feature of Parkinson’s Disease”

Parkinsonism & Related Disorders, Journal Year: 2025, Volume and Issue: unknown, P. 107280 - 107280

Published: Jan. 1, 2025

Language: Английский

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Biomarkers in Parkinson’s Disease

Neurologic Clinics, Journal Year: 2025, Volume and Issue: 43(2), P. 229 - 248

Published: Jan. 22, 2025

Language: Английский

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Brain network and energy imbalance in Parkinson’s disease: linking ATP reduction and α-synuclein pathology

Frontiers in Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 17

Published: Jan. 22, 2025

Parkinson’s disease (PD) involves the disruption of brain energy homeostasis. This encompasses broad-impact factors such as mitochondrial dysfunction, impaired glycolysis, and other metabolic disturbances, like disruptions in pentose phosphate pathway purine metabolism. Cortical hubs, which are highly connected regions essential for coordinating multiple functions, require significant due to their dense synaptic activity long-range connections. Deficits ATP production PD can severely impair these hubs. The imbalance also affects subcortical regions, including massive axonal arbors striatum substantia nigra pars compacta neurons, high demand. decline may result α -synuclein accumulation, autophagy-lysosomal system impairment, neuronal network breakdown accelerated neurodegeneration. We propose an “ATP Supply–Demand Mismatch Model” help explain pathogenesis PD. model emphasizes how deficits drive pathological protein aggregation, autophagy, degeneration key networks, contributing both motor non-motor symptoms.

Language: Английский

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Dual-Mode Ratiometric Electrochemical and Turn-on Fluorescent Probe for Reliably Detecting H2O2 in Parkinson's Disease Serum

Sensors and Actuators Reports, Journal Year: 2025, Volume and Issue: unknown, P. 100305 - 100305

Published: Feb. 1, 2025

Language: Английский

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Multimodal diagnostic tools and advanced data models for detection of prodromal Parkinson’s disease: a scoping review

BMC Medical Imaging, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 28, 2025

Abstract Background Parkinson’s Disease (PD) is a progressive neurodegenerative disorder characterized by the loss of dopaminergic neurons in substantia nigra pars compacta. PD diagnosed combination motor symptoms including bradykinesia, resting tremors, rigidity and postural instability. Prodromal stage preceding onset classic PD. The diagnosis prodromal remains challenging despite many available diagnostic modalities. Aim This scoping review aims to investigate explore current modalities used detect PD, focusing particularly on multimodal imaging analysis AI-based approaches. Methods We adhered PRISMA-SR guidelines for reviews. conducted comprehensive literature search at multiple databases such as PubMed, Scopus, Web Science, Cochrane Library from inception July 2024, using keywords related included studies based predefined inclusion exclusion criteria performed data extraction standardized form. Results 9 involving 567 patients with 35,643 control. Studies utilized various approaches neuroimaging techniques AI-driven models. sensitivity ranging 43 84% specificity up 96%. Neuroimaging AI technologies showed promising results identifying early pathological changes predicting onset. highest was achieved neuromelanin-sensitive model, while standard 10-s electrocardiogram (ECG) + Machine learning model. Conclusion Advanced models revealed detection However, their clinical application screening tool limited because lack validation. Future research should be directed towards Multimodal diagnosing Clinical trial number Not applicable.

Language: Английский

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Extracellular particles: emerging insights into central nervous system diseases

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 1, 2025

Extracellular particles (EPs), including extracellular vesicles (EVs) and non-vesicular (NVEPs), are multimolecular biomaterials released by cells that play a crucial role in intercellular communication. Recently, new subtypes of EPs associated with central nervous system (CNS), such as exophers supermeres have been identified. These provide perspectives for understanding the pathological progression CNS disorders confer potential diagnostic value liquid biopsies neurodegenerative diseases (NDs). Moreover, emerged promising drug delivery vehicles targeted platforms CNS-specific therapies. In this review, we delineate landscape EP their roles pathophysiology diseases. We also review recent advances EP-based diagnosis NDs highlight importance analytical single-particle resolution exploitation biomarkers. Furthermore, summarize application engineered EVs treatment outline underexplored NVEPs novel therapeutic agents.

Language: Английский

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Physiotherapy management of Parkinson’s disease in a tertiary hospital in Nigeria: a case report

Bulletin of Faculty of Physical Therapy, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 2, 2025

Abstract Parkinson’s disease (PD) is a progressive neurodegenerative disorder primarily characterized by the degeneration of dopaminergic neurons in substantia nigra, leading to motor impairments such as tremors, bradykinesia, and postural instability. It also affects cognitive functions, contributing difficulties movement control non-motor symptoms, including decline, sleep disturbances, emotional dysregulation. Although pharmacological treatments provide symptomatic relief, there limited evidence regarding effectiveness non-pharmacological interventions improving both outcomes. This case report details physiotherapy management 72-year-old male patient with stage 3 PD, highlighting structured 14-week program that targeted balance, coordination, function. The intervention, which incorporated personalized exercises training, resulted significant improvements tremor frequency, control, Remarkably, demonstrated progression from Hoehn Yahr 1, suggesting intensive can have profound impact on overall quality life (QOL). notable for its novel approach combining physical therapy PD patient, strategy not widely reported existing literature. Given lack curative findings underscore critical role interventions, physiotherapy, outcomes patients. highlights potential intensive, individualized programs promote neuroplasticity significantly enhance outcomes, offering new insights into holistic management.

Language: Английский

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Engineered Intelligent Electrochemical Biosensors for Portable Point-of-Care Diagnostics

Chemosensors, Journal Year: 2025, Volume and Issue: 13(4), P. 146 - 146

Published: April 16, 2025

The development of cost-effective, rapid-response, and user-friendly biosensing platforms has become paramount importance for achieving precise biomarker quantification in early disease detection. Implementing timely diagnostic interventions through accurate analysis not only significantly improves treatment outcomes but also enables effective management strategies, ultimately leading to substantial reductions patient mortality rates. These clinical imperatives have consequently driven the innovation portable point-of-care (POC) systems. Electrochemical biosensors are attractive diagnosis diseases due their low cost, simple operation, high sensitivity. This review examines prevalent material innovations electrode functionalization electrochemical platforms, with specific emphasis on translational applications early-stage included three important types: proteins, nucleic acids, small molecule metabolites. Furthermore, work proposes novel research trajectories next-generation biosensor development, advocating synergistic integration artificial intelligence-driven analytics, Internet Medical Things (IoMT)-enabled networks, advanced micro/nanofabrication techniques.

Language: Английский

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Advances in Understanding Biomarkers and Treating Neurological Diseases -Role of the Cerebellar Dysfunction and Emerging Therapies

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 102519 - 102519

Published: Sept. 1, 2024

Language: Английский

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