Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(11), P. 2795 - 2796
Published: Oct. 10, 2024
Language: Английский
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: July 25, 2024
Abstract Addressing the global disparity in cancer care necessitates development of rapid and affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical cancer, where molecular detection human papillomavirus (HPV) has emerged as an accurate screening method. However, implementing this transition low- middle-income countries been challenging due to high costs centralized facilities required current NA tests. Here, we present CreDiT (CRISPR Enhanced Digital Testing) on-site detection. The platform integrates i) a one-pot CRISPR strategy that simultaneously amplifies both target NAs analytical signals ii) robust fluorescent based on digital communication (encoding/decoding) technology. These features enable assay (<35 minutes) single streamlined workflow. We demonstrate sensitive cell-derived HPV DNA targets down copies identification types clinical brushing specimens ( n = 121).
Language: Английский
Citations
10
Small, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 13, 2025
Abstract Point‐of‐care (POC) pathogen detection is highly desirable in diverse fields such as infectious disease diagnosis, food safety testing, and environmental monitoring. Herein, the study seeks to address this critical need by developing an automated microfluidic photothermal quantitative polymerase chain reaction (AMP‐qPCR) system a greatly simplified format. A key element of AMP‐qPCR architecture that combines design clockwork‐like, magnetically‐driven multi‐chamber cartridge with use cheap black tape beneath PCR chamber fast photothermal‐responsive engine. This not only enables unprocessed sample be lysed, purified, subjected real‐time fluorescence ultracompact autonomous manner but also eliminates for sophisticated photonic material/device fabrication frequently required performing ultrafast PCR. It shown can accomplish high‐efficient bacterial DNA extraction within 18.5 min, enabling accurate quantification bacteria concentration from 10 8 2 CFU·mL −1 . Furthermore, its practical applicability demonstrated detecting Neisseria gonorrhoeae sexually transmitted infection‐suspected patients using clinical urine cervical swab specimens, exhibiting matched performance benchtop machine. The presented platform enhances availability POC molecular diagnostics on‐site in‐home testing.
Language: Английский
Citations
1
BME Frontiers, Journal Year: 2025, Volume and Issue: 6
Published: Jan. 1, 2025
Human papillomavirus (HPV) is the most common virus for genital tract infections. Cervical cancer ranks as fourth prevalent globally, with over 99% of cases in women attributed to HPV infection. This infection continues pose an ongoing threat public health. Therefore, development rapid, high-throughput, and sensitive detection platforms important, especially regions limited access advanced medical resources. CRISPR-based biosensors, a promising new method nucleic acid detection, are now rapidly widely used basic applied research have received much attention recent years diagnosis treatment. In this review, we discuss mechanisms functions CRISPR-Cas system, focusing on its applications diagnostics. The review covers CRISPR technologies such CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, along amplification methods, signal output systems, point-of-care testing (POCT) strategies. comprehensive overview highlights versatility potential detection. We also numerous biosensors developed since introduction detect HPV. Finally, some challenges faced by system.
Language: Английский
Citations
0
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: March 31, 2025
Abstract The early detection of high‐risk human papillomavirus (HR‐HPV) is crucial for the assessment and improvement prognosis in cervical cancer. However, existing PCR‐based screening methods suffer from inadequate accessibility, which dampens enthusiasm among grassroots populations, especially resource‐limited areas, contributes to persistently high mortality rate Here, a portable system proposed multiplexed nucleic acid detection, termed R‐CHIP , that integrates R ecombinase polymerase amplification (RPA), C RISPR H and‐driven microfluidics, an artificial I ntelligence P latform. can go sample pre‐processing results readout less than hour with simple manual operation. Optimized sensitivity 10 −17 M HPV‐16 −18 HPV‐18, has accuracy over 95% 300 tests on clinical samples. In addition, smartphone microimaging combined ResNet‐18 deep learning model used improve efficiency convenience system, initial prediction accuracies 96.0% 98.0% respectively. R‐CHIP, as user‐friendly intelligent platform, great potential community‐level HR‐HPV resource‐constrained settings, prevention diagnosis other diseases.
Language: Английский
Citations
0
Methods, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
Citations
0
TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118296 - 118296
Published: May 1, 2025
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: May 13, 2025
In vitro diagnostics require the accurate detection of disease-associated target biomolecules at ultralow concentrations. A multimode sensing strategy is considered as a potential method for in diagnosis because it allows cross-validation test results through data complementation and self-calibration, provides double confirmation. Here, we present CRISPR/Cas12a-powered trimode biosensor (CPTMB) ultrasensitive reliable analysis miRNA. Briefly, presence miRNA initiates rolling circle extension-driven loop-mediated isothermal amplification (R-LAMP), which subsequently activates trans-cleavage activity CRISPR/Cas12a. Then, hairpin probe (HP) biogate on nucleic acid-functionalized MB@Fe-MOF signal was degraded by Cas12a, leading to release methylene blue (MB) molecules encapsulated within Fe-MOF nanocarriers. Due capability MB generate output responses across three distinct modes: electrochemical (EC), fluorescence (FL), ultraviolet-visible spectroscopy (UV-vis), trimodal system achieved. Benefiting from efficient capabilities R-LAMP CRISPR/Cas12a, this enables rapid femtomolar levels 70 min. Furthermore, modes cross-validate one another, thereby enhancing reliability analysis. More importantly, platform has been successfully applied real samples, are good agreement with those standard RT-qPCR, indicating its great clinical early-stage cancer.
Language: Английский
Citations
0
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118054 - 118054
Published: Nov. 1, 2024
Language: Английский
Citations
3
Chinese Journal of Lasers, Journal Year: 2025, Volume and Issue: 52(3), P. 0307202 - 0307202
Published: Jan. 1, 2025
Citations
0
Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(11), P. 2795 - 2796
Published: Oct. 10, 2024
Language: Английский
Citations
1