Harnessing Nanomaterials For Enhanced DNA‐Based Biosensing And Therapeutic Performance DOI Open Access

Xumin Pan,

Xiaoman Zhao,

Yanhong Lu

et al.

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

The integration of nanomaterials with DNA-based systems has emerged as a transformative approach in biosensing and therapeutic applications. Unique features DNA, like its programmability specificity, complement the diverse functions nanomaterials, leading to creation advanced for detecting biomarkers delivering treatments. Here, we review developments DNA-nanomaterial conjugates, emphasizing their enhanced functionalities potential across various biomedical We first discuss methodologies synthesizing these distinguishing between covalent non-covalent interactions. then categorize DNA-nanomaterials conjugates based on properties DNA involved, respectively. probes are classified by application into or uses, and, several highlighted recent progress living biological. Finally, current challenges future prospects this field, anticipating that significant will greatly enhance precision medicine.

Language: Английский

Imaging-guided Companion Theranostics: Ushering in A New Era of Personalized Cancer Care DOI Creative Commons
Meng Li, Yumeng Wu, Jing Lin

et al.

Nano Biomedicine and Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

1
Multielement Doping Engineered Iron Oxide Nanoparticles: Enabling the Shift from Negative to Positive MRI Contrast for Enhanced Diagnostic Precision DOI Open Access
Chichong Lu,

Yingjie Bao,

Zihan Fei

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Abstract Contrast‐enhanced magnetic resonance imaging (CE‐MRI) is a crucial tool for the diagnosis and management of various diseases globally. Iron oxide nanoparticles with sizes less than 5 nm are expected to address long‐term toxicity brain accumulation issues associated clinical gadolinium‐based T 1 contrast agents (GBCAs) due their non‐toxicity biodegradability. However, synthesizing sub‐5‐nanometer particles presents significant challenges that complicate translation. Herein, traditional iron oxide‐based negative ( 2 ) into positive transformed an all‐in‐one multielement doping strategy developed. Multiple elements crystals introduced form engineered (MDE‐IONPs) surfaces flexible hydrophilic ligands subsequently modified. It shown Ni (II) Gd (III) can effectively enhance efficacy, reducing clearance rates, enabling controlled synthesis. Ultimately, implementation co‐engineering yield longitudinal relaxivity up 14.7 mM −1 s even as large 9 nm, improvement approximately 300% over GBCAs. Combined stability, biosafety, both in vitro vivo results suggest favorable advancing development next‐generation safe MRI agents.

Language: Английский

Citations

1
PAMAM-Based DNA Fluorescence Nanoprobe for Rapid Whole Cellular APE1 Detection and Imaging DOI
Huimin Li,

Xinru Yuan,

Mei Ren

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/ref-1, APE1), a vital protein for DNA repair and cellular redox regulation, is frequently overexpressed in tumor cells, underscoring the importance of developing sensitive detection methods early cancer diagnosis. However, rapid visualization nuclear APE1 cells are still challenging. In this study, we successfully developed novel fluorescent nanoprobe based on polyamidoamine (PAMAM) cytoplasmic APE1. The PAMAM surface was modified with arginine (Arg), named PR, its hydrophobic core encapsulated 1,6,7,12-tetrachloroperylene tetracarboxylic acid dianhydride (TA) dye to construct nanoparticles (TPR). Furthermore, an APE1-responsive dsDNA (SP) linked TPR surface, containing apurinic/apyrimidinic sites (AP sites) black hole quencher 2 (BHQ2) ensuring that fluorescence remains off absence TPR-SP exhibited range 0.125-25 U mL-1 limit as low 0.03 mL-1. Compared Arg-free nanoprobes (TP-SP), significantly accelerated endocytosis penetration, reducing time one-quarter (from 0.5 h). Notably, signal whole nucleus can also be detected. Thus, achieves enrichment amplification signals, leading highly detection. This innovative efficient method greatly expands technological means

Language: Английский

Citations

0
An Enzyme-Triggered Au–Se Nanodevice for Precise Imaging of MicroRNA DOI
Ruyue Wei, Shuqi Wang,

Yingbo Pan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

In situ imaging of microRNA (miRNA) in tumor cells is vital for clinical diagnosis and pathological research. However, achieving high-precision always limited by undesirable background signals. Herein, we introduced a gold-selenium-based nanodevice (AuSeND) high-fidelity miRNA via enzyme-triggered catalytic hairpin assembly (CHA). This system employs an enzyme-activatable CHA circuit, constructed extending short tail at the 3' end H1 with apurinic/apyrimidinic (AP) site. The circuit components are connected to surface AuNPs Au-Se bonds, forming nanodevice. It remains inactive normal cells, while activated endonuclease 1 (APE1) cytoplasm, generating fluorescence signal under stimulation imaging. developed AuSeND enables cancer cell-selective improves signal-to-noise ratio combining high stability bond specific regulation APE1 enzyme, offering strong potential diagnostic applications.

Language: Английский

Citations

0
Molecular Strategies for Constructing Epoxy‐Type Afterglow Probes in Disease Diagnosis and Treatment DOI

Maocheng Zuo,

Yidan Hu,

L. Xiao

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Abstract Epoxy‐type afterglow luminescence is a cascade reaction of photochemical defects caused as result the formation and release epoxy intermediates, exhibiting continuous phenomenon that remains intrinsic after excitation, with extremely impressive signal‐to‐background ratio (SBR) level in vivo. Afterglow based on this epoxy‐type mechanism ideally suited for applications developments diagnosis treatment biological diseases. In tutorial review, work aims to deeply elaborate mechanisms materials present application imaging Lastly, future perspectives potential challenges technology preclinical diagnostic, therapeutic, predictive analyses, well clinical translations are discussed.

Language: Английский

Citations

0
Versatile and Programmable Dual-Mode Logic Gold Nanoflares for Intracellular Correlated DNA Repair Enzymes Imaging DOI

Liannishang Li,

Lei Huang, M. Li

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117501 - 117501

Published: April 1, 2025

Language: Английский

Citations

0
Vascular Magnifier for Ultrahigh-Resolution Visualization of Cerebral Vessels in Vivo DOI
Bingjie Li, Jinbin Pan,

Ruijie Zhang

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123356 - 123356

Published: April 1, 2025

Language: Английский

Citations

0
A dark-state–dominated photochemical upconversion afterglow via triplet energy transfer relay DOI Creative Commons

Hang Yuan,

Kuangshi Sun,

Xianlong Su

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(17)

Published: April 25, 2025

Photochemical afterglow materials have drawn considerable attention due to their attractive luminescent properties and great application potential. Considering the classical photochemical always exhibit poor luminescence, it is urgent gain fundamental understanding of main limiting factors. Here, we identified existence a dark-state triplet in process, an overwhelming percentage ~98.5% was revealed for this non-emissive state. Guided by these observations, proposed activate unprecedented energy transfer relay simultaneously harness singlet energy. Consequently, upconverted material constructed with amazing luminescence performance albeit its moderate fluorescence emission property. The generality strategy evidenced adaptation similar emitters varied wavelengths. optimized enabled time-gated upconversion bioimaging under ultralow-power excitation. This study not only reveals pathways but also paves way rational design bright ultralong lifetime.

Language: Английский

Citations

0
Harnessing Nanomaterials For Enhanced DNA‐Based Biosensing And Therapeutic Performance DOI Open Access

Xumin Pan,

Xiaoman Zhao,

Yanhong Lu

et al.

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

The integration of nanomaterials with DNA-based systems has emerged as a transformative approach in biosensing and therapeutic applications. Unique features DNA, like its programmability specificity, complement the diverse functions nanomaterials, leading to creation advanced for detecting biomarkers delivering treatments. Here, we review developments DNA-nanomaterial conjugates, emphasizing their enhanced functionalities potential across various biomedical We first discuss methodologies synthesizing these distinguishing between covalent non-covalent interactions. then categorize DNA-nanomaterials conjugates based on properties DNA involved, respectively. probes are classified by application into or uses, and, several highlighted recent progress living biological. Finally, current challenges future prospects this field, anticipating that significant will greatly enhance precision medicine.

Language: Английский

Citations

0